pIIIa (AC_000006) Virus Tagged ORF Clone

CAT#: VC100409

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus D], codon optimized for human cell expression, AP_000146



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CNY 6,840.00


货期*
4周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100409 represents NCBI reference of AP_000146 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCAGGCTCCTGATCCGGCCATCAGGGCAGCGCTCCAGAGCCAGCCTTCTGGACTGGCATCCGATG
ACTGGGAGGCAGCGATGCAACGGATTATGGCATTGACCACTCGAAATCCTGAGAGCTTCAGGCAGCAGCC
TCAGGCCAACCGGTTGAGCGCCATTTTGGAGGCCGTCGTGCCAAGTAGGACCAACCCAACCCACGAGAAA
GTACTGGCGATCGTAAATGCCCTGGCGGAAAATAAGGCAATACGCCCCGACGAAGCGGGGCTTGTCTACA
ACGCTCTGCTCGAGAGGGTGGGCAGGTATAATTCCACTAATGTGCAGTCCAACTTGGATAGATTGGTGAC
CGATGTGCGGGAGGCCGTGGCCCAGAGGGAACGGTTCAAAAATGAGGGCCTGGGAAGCCTTGTGGCATTG
AACGCCTTTCTCGCTACTCAGCCTGCCAACGTGCCCCGGGGGCAAGATGACTATACAAACTTCATCAGTG
CCCTGAGACTGATGGTCACCGAGGTCCCACAGTCAGAAGTTTACCAGTCCGGTCCAGACTACTTTTTCCA
GACAAGCCGGCAGGGGCTTCAGACAGTTAACCTGAGCCAGGCATTTAAAAACCTGAGGGGTCTCTGGGGC
GTGCAAGCTCCCGTCGGGGACAGATCCACCGTTTCATCTCTCCTGACCCCCAATTCACGACTGTTGCTGC
TGCTCATAGCTCCATTCACTGATTCCGGTTCAGTGAACAGGAACAGCTACCTGGGACATCTGCTGACTTT
GTACAGAGAGGCTATTGGACAGGCCCAAGTAGATGAGCAAACTTTCCAGGAAATCACAAGCGTGAGTAGA
GCCCTGGGTCAGAACGACACGGACAGTCTCCGAGCCACCCTGAACTTTCTGCTCACCAACCGGCAGCAGA
AGATTCCCGCCCAGTATGCATTGAGTGCCGAGGAGGAAAGGATACTTAGGTATGTTCAGCAAAGCGTGGG
GCTTTTTCTCATGCAAGAAGGGGCAACCCCCTCTGCAGCCCTCGATATGACAGCTCGAAATATGGAACCT
AGTATGTATGCTGCGAACCGCCCATTTATCAACAAGCTGATGGACTACCTTCATAGAGCCGCCTCTATGA
ATTCAGATTACTTTACCAACGCCATTCTCAACCCCCACTGGCTTCCTCCACCAGGCTTTTACACAGGTGA
GTACGACATGCCTGATCCAAACGATGGATTTCTGTGGGATGATGTCGACTCCGCTGTTTTCTCTCCAACT
CTCCAGAAACGGCAGGAAGCGGTCCGGACTCCAGCCAGTGAGGGCGCAGTGGGACGGTCCCCATTCCCCT
CCTTGGGAAGCCTGCACTCCCTGCCTGGCAGTGTGAATAGCGGGAGGGTCAGCAGACCCCGCCTTCTGGG
AGAAGATGAATATCTCAATGATTCTCTCCTCCAACCTCCAAGAGTAAAGAATGCGATGGCCAACAACGGC
ATAGAATCACTCGTTGACAAGCTGAATCGGTGGAAGACCTACGCCCAGGACCATAGAGAGCCCGCTCCTG
CTCCGCGGCGCCAGCGGCACGATAGGCAACGCGGACTCGTATGGGACGATGAAGACTCTGCCGATGACTC
TAGCGTGCTCGACCTGGGCGGGTCTGGCGGCGTAAATCCCATCTCAAGGATACTGCAGCCCAAGTTGGGC
CGCCGGATGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100409 representing AP_000146
Red=Cloning sites Green=Tags

MQQAPDPAIRAALQSQPSGLASDDWEAAMQRIMALTTRNPESFRQQPQANRLSAILEAVVPSRTNPTHEK
VLAIVNALAENKAIRPDEAGLVYNALLERVGRYNSTNVQSNLDRLVTDVREAVAQRERFKNEGLGSLVAL
NAFLATQPANVPRGQDDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLRGLWG
VQAPVGDRSTVSSLLTPNSRLLLLLIAPFTDSGSVNRNSYLGHLLTLYREAIGQAQVDEQTFQEITSVSR
ALGQNDTDSLRATLNFLLTNRQQKIPAQYALSAEEERILRYVQQSVGLFLMQEGATPSAALDMTARNMEP
SMYAANRPFINKLMDYLHRAASMNSDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSAVFSPT
LQKRQEAVRTPASEGAVGRSPFPSLGSLHSLPGSVNSGRVSRPRLLGEDEYLNDSLLQPPRVKNAMANNG
IESLVDKLNRWKTYAQDHREPAPAPRRQRHDRQRGLVWDDEDSADDSSVLDLGGSGGVNPISRILQPKLG
RRMF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000006
ORF Size 1692 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000006.1, AP_000146
RefSeq ORF 1692 bp
MW 62.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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