pIIIa (AC_000006) Virus Tagged ORF Clone
CAT#: VC100409
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus D], codon optimized for human cell expression, AP_000146
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CNY 6,840.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100409 represents NCBI reference of AP_000146 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCAGGCTCCTGATCCGGCCATCAGGGCAGCGCTCCAGAGCCAGCCTTCTGGACTGGCATCCGATG ACTGGGAGGCAGCGATGCAACGGATTATGGCATTGACCACTCGAAATCCTGAGAGCTTCAGGCAGCAGCC TCAGGCCAACCGGTTGAGCGCCATTTTGGAGGCCGTCGTGCCAAGTAGGACCAACCCAACCCACGAGAAA GTACTGGCGATCGTAAATGCCCTGGCGGAAAATAAGGCAATACGCCCCGACGAAGCGGGGCTTGTCTACA ACGCTCTGCTCGAGAGGGTGGGCAGGTATAATTCCACTAATGTGCAGTCCAACTTGGATAGATTGGTGAC CGATGTGCGGGAGGCCGTGGCCCAGAGGGAACGGTTCAAAAATGAGGGCCTGGGAAGCCTTGTGGCATTG AACGCCTTTCTCGCTACTCAGCCTGCCAACGTGCCCCGGGGGCAAGATGACTATACAAACTTCATCAGTG CCCTGAGACTGATGGTCACCGAGGTCCCACAGTCAGAAGTTTACCAGTCCGGTCCAGACTACTTTTTCCA GACAAGCCGGCAGGGGCTTCAGACAGTTAACCTGAGCCAGGCATTTAAAAACCTGAGGGGTCTCTGGGGC GTGCAAGCTCCCGTCGGGGACAGATCCACCGTTTCATCTCTCCTGACCCCCAATTCACGACTGTTGCTGC TGCTCATAGCTCCATTCACTGATTCCGGTTCAGTGAACAGGAACAGCTACCTGGGACATCTGCTGACTTT GTACAGAGAGGCTATTGGACAGGCCCAAGTAGATGAGCAAACTTTCCAGGAAATCACAAGCGTGAGTAGA GCCCTGGGTCAGAACGACACGGACAGTCTCCGAGCCACCCTGAACTTTCTGCTCACCAACCGGCAGCAGA AGATTCCCGCCCAGTATGCATTGAGTGCCGAGGAGGAAAGGATACTTAGGTATGTTCAGCAAAGCGTGGG GCTTTTTCTCATGCAAGAAGGGGCAACCCCCTCTGCAGCCCTCGATATGACAGCTCGAAATATGGAACCT AGTATGTATGCTGCGAACCGCCCATTTATCAACAAGCTGATGGACTACCTTCATAGAGCCGCCTCTATGA ATTCAGATTACTTTACCAACGCCATTCTCAACCCCCACTGGCTTCCTCCACCAGGCTTTTACACAGGTGA GTACGACATGCCTGATCCAAACGATGGATTTCTGTGGGATGATGTCGACTCCGCTGTTTTCTCTCCAACT CTCCAGAAACGGCAGGAAGCGGTCCGGACTCCAGCCAGTGAGGGCGCAGTGGGACGGTCCCCATTCCCCT CCTTGGGAAGCCTGCACTCCCTGCCTGGCAGTGTGAATAGCGGGAGGGTCAGCAGACCCCGCCTTCTGGG AGAAGATGAATATCTCAATGATTCTCTCCTCCAACCTCCAAGAGTAAAGAATGCGATGGCCAACAACGGC ATAGAATCACTCGTTGACAAGCTGAATCGGTGGAAGACCTACGCCCAGGACCATAGAGAGCCCGCTCCTG CTCCGCGGCGCCAGCGGCACGATAGGCAACGCGGACTCGTATGGGACGATGAAGACTCTGCCGATGACTC TAGCGTGCTCGACCTGGGCGGGTCTGGCGGCGTAAATCCCATCTCAAGGATACTGCAGCCCAAGTTGGGC CGCCGGATGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100409 representing AP_000146
Red=Cloning sites Green=Tags MQQAPDPAIRAALQSQPSGLASDDWEAAMQRIMALTTRNPESFRQQPQANRLSAILEAVVPSRTNPTHEK VLAIVNALAENKAIRPDEAGLVYNALLERVGRYNSTNVQSNLDRLVTDVREAVAQRERFKNEGLGSLVAL NAFLATQPANVPRGQDDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLRGLWG VQAPVGDRSTVSSLLTPNSRLLLLLIAPFTDSGSVNRNSYLGHLLTLYREAIGQAQVDEQTFQEITSVSR ALGQNDTDSLRATLNFLLTNRQQKIPAQYALSAEEERILRYVQQSVGLFLMQEGATPSAALDMTARNMEP SMYAANRPFINKLMDYLHRAASMNSDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSAVFSPT LQKRQEAVRTPASEGAVGRSPFPSLGSLHSLPGSVNSGRVSRPRLLGEDEYLNDSLLQPPRVKNAMANNG IESLVDKLNRWKTYAQDHREPAPAPRRQRHDRQRGLVWDDEDSADDSSVLDLGGSGGVNPISRILQPKLG RRMF TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | AC_000006 |
ORF Size | 1692 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | AC_000006.1, AP_000146 |
RefSeq ORF | 1692 bp |
MW | 62.6 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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