NS1 (NC_012042) Virus Tagged ORF Clone

CAT#: VC100544

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for NS1 [Human bocavirus 2], codon optimized for human cell expression, YP_002586773



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CNY 7,700.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100544 represents NCBI reference of YP_002586773 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTCTCCGCACCTGTAATAAGGGCATTCTCTCAGCCAGCATTTACTTATGTCGTTAAATTCCCAT
ATGAGAATTGGAAAGAGGAAGAGCACCTTCTGTGGAGCCTTCTGGCCCCAGGGACCGAAAGGCTGATGAT
CCAGCTCCGAAATTGCGCCCCTCATCCCGAAGACGATCCAGTCCGAGAGGACATACTGTGCTCTCTTGCA
GACCAGCACTATGGAGCAATCTTTGCAAAAGCTTGCTACATTGCTACAACAACCCTCATGGGCCAAAAGC
AACGCACCCCATTTCCTCGGTGCGATATCATATGCCAATCAGAGATCGGTTCAGAGCACCTGCATTGTCA
CATACTCGTGGGCGGGGCCGGACTCAGTAAAAGAAATGCGAAGATATCACGAGCCACCCTTCTCGGCCTT
GTCATGGCTGAACTGACACAGCGGTGCAAACAGTTGCTTGCATTGAGACCTTTCGAGCCCGCCGAAGCTA
ATATTTTCCACCTGCTTAAACGAATTGAGAGAGAAGCTTGGAGTGGGCACACTGGTAACTGGGTGCAGAT
TCTTCAGTATAAGGACAAAAGGGGTGATCTCCATGCCCAACCAATTGACCCCCTGAGATTCCTCAAGCAT
TACATCCTCCCCAAGAATAGGCTTATTTCTCCAAGCAGCAAGCCTGACGTGTGCACAACCCCAGACAACT
GGTTTATCCTGGCTGACAAGACCTACGCCCACACTATAATCAACGGCCTCCCCTTGCTGGAACACAATCG
GAAGGCCTACCTCCAGGAGCTCGAGTCCGAGGTGATCCCAGGGCCTTCTACAATGGCTTTTGGAGGTAGG
GGGGCGTGGGAGCAGCTGCCCGAGGTGGGCGAGCAACGCTTGATAACAAGCAACGCTAGCACCGCATACA
AAGCCAATAAGAAAGAGAAGCTGATGCTGAATTTGCTGGATAAGTGCGACGAACTGAATCTGCTGGTCTA
TGAGGACCTGGTGTCTGCCTGCCCTGACCTGCTCCTCATGCTTGAGGGCCAACCTGGCGGCGCTCGACTC
ATCGAACAAGTGTTGGGCATGCATCACATAAAAGTTTGCGCAAAATATACCGCCCTTACCTTCTTGTTCC
ACTTGCATCCTGATCAGCTGCTGACTTCTAACAACAAAGCTCTGAAACTGCTCCTGATACAGGGATACAA
CCCACTTCAGGTAGGACACGCGATCTGCTGTGTGCTCAATAAGCAGATGGGAAAGCAGAATACTATCTGT
TTTTACGGACCTGCTTCCACCGGCAAGACCAATTTTGCAAAGGCCATCGTGCAGGGCGTGAGGCTGTATG
GATGTGTGAACCACCTCAATAAGGGTTTTGTCTTTAATGATTGCCGGCAGCGACTGATAATCTGGTGGGA
GGAGTGTTTGATGCACCAAGATTGGGTGGAGCCCGCTAAATGTATCCTGGGCGGCACTGAATGTAGGATT
GATGTGAAGCACAAGGATTCCGTTCTGCTCCAGCAAACGCCTGTGATCATAAGTACTAACCATGACATTT
ACTCTGTAGTCGGCGGAAACACTGTGTCCCATGTCCATGCTGCTCCCCTGAAGGAAAGGGTACTTCAGCT
CAACTTTATGAAGCAACTGCCTCAGACCTTTGGCGAAATCAGCCCCGTGGAAATCGCCGAACTGCTCCAG
TGGTGCTTCAATGAGTACGAGTGCACGCTGACCGGATTTAAACAGAAATGGAACCTCGACAAAGTGCCCA
ATTCCTTTCCCCTGGGCGACCTCTGCCCTACCCACAGCCAGGACTACACACTGCACGAGAATGGGTTCTG
CACTGACTGCGGCGGCTATCTGCCCCATTCTGCTGACGACTTCGTATACACCGATGTGGCCTCAGAGACT
ACCAGTGGCGATTGCGATCCAGGGCGACTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100544 representing YP_002586773
Red=Cloning sites Green=Tags

MAFSAPVIRAFSQPAFTYVVKFPYENWKEEEHLLWSLLAPGTERLMIQLRNCAPHPEDDPVREDILCSLA
DQHYGAIFAKACYIATTTLMGQKQRTPFPRCDIICQSEIGSEHLHCHILVGGAGLSKRNAKISRATLLGL
VMAELTQRCKQLLALRPFEPAEANIFHLLKRIEREAWSGHTGNWVQILQYKDKRGDLHAQPIDPLRFLKH
YILPKNRLISPSSKPDVCTTPDNWFILADKTYAHTIINGLPLLEHNRKAYLQELESEVIPGPSTMAFGGR
GAWEQLPEVGEQRLITSNASTAYKANKKEKLMLNLLDKCDELNLLVYEDLVSACPDLLLMLEGQPGGARL
IEQVLGMHHIKVCAKYTALTFLFHLHPDQLLTSNNKALKLLLIQGYNPLQVGHAICCVLNKQMGKQNTIC
FYGPASTGKTNFAKAIVQGVRLYGCVNHLNKGFVFNDCRQRLIIWWEECLMHQDWVEPAKCILGGTECRI
DVKHKDSVLLQQTPVIISTNHDIYSVVGGNTVSHVHAAPLKERVLQLNFMKQLPQTFGEISPVEIAELLQ
WCFNEYECTLTGFKQKWNLDKVPNSFPLGDLCPTHSQDYTLHENGFCTDCGGYLPHSADDFVYTDVASET
TSGDCDPGRL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_012042
ORF Size 1920 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_012042.1, YP_002586773
RefSeq ORF 1920 bp
Locus ID 7755626
MW 72.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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