VP1 (NC_012564) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100550 represents NCBI reference of YP_002808456 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCCCATTAAGCGCCAGCCAGGGGGATGGGTCCTCCCCGGCTATAAGTATCTTGGGCCTTTTAACC CACTGGATAATGGCGAGCCTGTGAATAAAGCAGATCGAGCGGCTCAAAGTCACGATAAGTCCTATAGCGA GCTGATCAAGTCAGGTAAGAACCCTTATCTGTACTTCAACAAAGCAGACGAGAAATTCATCGACGACCTC AAAAATGACTGGTCCTTGGGAGGCATCATAGGATCCAGCTTCTTTAAGTTGAAGAGGGCTGTGGCTCCCG CTCTGGGCAACAAAGAGAGAGCCCAGAAACGACACTTTTATTTTGCAAATTCAAATAAGGGCGCAAAGAA ATCCAAGAACAACGAACCTAAGCCAAGTACCTCTAAGATGAGCGAAAACGAGATTCAGGATCAGCAGCCC TCCGAGCCCAATGATGGTCAGCGCGGGGGGGGAGGTGGAGCTACCGGCTCTGTCGGTGGGGGGAAGGGCA GTGGAGTGGGAATCTCTACAGGAGGATGGGTGGGGGGGAGCTACTTTACAGACTCTTATGTGATCACTAA AAACACGCGGCAGTTTCTGGTTAAAATCCAAAATAATCACCAGTACAAGACCGAAAGCATTATTCCTAGC AACGGAGGAGGAAAAAGCCAACGATGTGTTAGCACGCCCTGGTCTTACTTTAATTTCAATCAATATTCTT CTCACTTCAGCCCACAGGACTGGCAACGGCTCACAAACGAATACAAGCGGTTTAGACCGAAGGGAATGCA CGTCAAAATTTACAATTTGCAGATCAAACAGATTCTTAGCAATGGCGCAGACGTGACCTACAACAATGAT CTCACAGCCGGAGTGCACATTTTCTGCGATGGCGAGCACGCTTATCCCAACGCCACACACCCATGGGATG AAGATGTTATGCCCGAGTTGCCTTACCAAACCTGGTACCTTTTCCAGTACGGCTACATCCCTACCATTCA TGAACTGGCTGAGATGGAGGATAGTAATGCTGTTGAGAAAGCAATCGCCCTGCAGATACCTTTCTTTATG CTCGAGAACTCCGACCACGAGGTTCTCAGGACAGGCGAGTCAGCCGAATTCAATTTCAATTTCGACTGTG AGTGGATAAATAACGAGCGGGCTTTCATACCACCCGGACTCATGTTTAACCCTCTCGTGCCGACCCGAAG GGCACAATACATTCGCAGAAATGGAAACACTCAAGCCAGTACATCTAGGGTGCAACCTTACGCGAAACCC ACTTCCTGGATGACAGGCCCTGGACTGTTGAGCGCACAAAGGGTCGGACCCGCCGCTTCTGATACAGCCG CCTGGATGGTGGGCGTCGATCCTGAAGGGGCCAACATCAACTCAGGTAGAGCTGGCGTTAGTTCTGGATT CGACCCTCCAGCCGGATCCCTTAGGCCAACAGATCTCGAGTACAAAGTGCAGTGGTACCAGACGCCCGCC GGCACAAATAATGATGGGAACATAATTTCCAATCCCCCGCTGTCTATGCTCCGGGACCAGACACTGTATC GGGGCAACCAGACCACGTATAACCTGTGTAGCGACGTTTGGATGTTCCCAAATCAGATCTGGGACAGATA TCCAGTTACGCGGGAGAACCCAATTTGGTGTAAACAGCCCCGGTCTGACAAGCATACCACTATCGATCCA TTTGACGGTTCCATAGCCATGGATCATCCTCCTGGTACTATCTTTATAAAAATGGCCAAGATCCCAGTGC CTTCAAACAATAACGCAGATAGCTACCTGAACATATATTGCACCGGTCAGGTTTCATGTGAGATCGTGTG GGAGGTGGAAAGGTACGCAACTAAGAATTGGCGCCCAGAACGCCGACACACAGCCCTTGGTCTGGGAATA GGTGGCGCCGATGAAATTAATCCCACCTACCATGTCGATAAGAACGGAGCCTATATTCAGCCAACTACCT GGGATATGTGCTTCCCAGTGAAAACCAATATCAATAAGGTATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100550 representing YP_002808456 Red=Cloning sites Green=Tags MPPIKRQPGGWVLPGYKYLGPFNPLDNGEPVNKADRAAQSHDKSYSELIKSGKNPYLYFNKADEKFIDDL KNDWSLGGIIGSSFFKLKRAVAPALGNKERAQKRHFYFANSNKGAKKSKNNEPKPSTSKMSENEIQDQQP SEPNDGQRGGGGGATGSVGGGKGSGVGISTGGWVGGSYFTDSYVITKNTRQFLVKIQNNHQYKTESIIPS NGGGKSQRCVSTPWSYFNFNQYSSHFSPQDWQRLTNEYKRFRPKGMHVKIYNLQIKQILSNGADVTYNND LTAGVHIFCDGEHAYPNATHPWDEDVMPELPYQTWYLFQYGYIPTIHELAEMEDSNAVEKAIALQIPFFM LENSDHEVLRTGESAEFNFNFDCEWINNERAFIPPGLMFNPLVPTRRAQYIRRNGNTQASTSRVQPYAKP TSWMTGPGLLSAQRVGPAASDTAAWMVGVDPEGANINSGRAGVSSGFDPPAGSLRPTDLEYKVQWYQTPA GTNNDGNIISNPPLSMLRDQTLYRGNQTTYNLCSDVWMFPNQIWDRYPVTRENPIWCKQPRSDKHTTIDP FDGSIAMDHPPGTIFIKMAKIPVPSNNNADSYLNIYCTGQVSCEIVWEVERYATKNWRPERRHTALGLGI GGADEINPTYHVDKNGAYIQPTTWDMCFPVKTNINKVL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_012564
ORF Size	2004 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_012564.1, YP_002808456
RefSeq ORF	2004 bp
Locus ID	7768238
MW	74.8 kDa

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