NS2 (NC_012729) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100553 represents NCBI reference of YP_002916059 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTTAGCGCACCGGTCCTTAGGGCCTTCAGCCAGCCAACCTTCACATATGTGATCAAGTTCCCTT ATAATAATTGGAAAGAAGACGAGCACCTGCTGTGGTCCCTGCTGGCCCCAGGAACTGAATCACTGATGAT TCAGCTGAAAAACTGCGCGCCGCATCCCGAGGACGACCCTATTAGAGAGGATATCCTGTGTAGTCTGGCC GACCTGCATTACGGAGCCGTGTTTGCGAAGGCTTGTTATATCGCCACCAGCACACTTATGGGCCAGAAGC AGCGCACCCTGTTTCCCCGGTGTGACATTGTCTGCCAAAGCGAGATAGGGTCCGACTTTCTGCATTGCCA CATTCTGGTGGGCGGTGCAGGTCTGTCCAAACGGAATGCCAAAATTAGCCGGGCTACTCTCTTGGGACTG GTGATGGCTGAACTTACGCAGCGCTGTAAGCTGTTGCTCGCCCACAGACCGTTCGAGCCAGCAGAGGCTA CCATTTATCATGAGCTTAAAAGGATCGAAAGAGAAGCCTGGTCAGGCCATACCGGCAATTGGGTCCAGAT CTTGCAATACAAAGACAAGCGGGGGGACCTGCACGCCCAACCCATCGACCCACTGCGCTTTCTGAAACAC TATATCCTCCCAAAGAATAGACTGATATCACCGAGCAGCAAGCCTGATGTGTGTACGAGTCCCGATAACT GGTTCATCCTGGCCGATAAAACATACTCTCATACCATAATCAACGGCCTCCCACTGCTGGAGCGGAACAG AAAGGCTTACCTGCAGGAGCTGGAATCCGAGGTGATACCTGGACCTTCAGCAATGGCCTTCGGAGGTAGA GGGGCATGGGAGCAACTCCCTGAGGTTGGGGAACAGCGACTGATCACTAGCAACACATCCACTGCTTACA AAGCAAATAAGAAAGAGAAATTGATGCTCAATCTTCTGGATAAATGCGACGAGCTGAATCTCCTTGTGTA CGAGGATCTTGTCTCCGCGTGCCCGGATCTGTTGCTGATGCTCGAGGGTCAGCCAGGAGGGGCTCGATTG ATAGAACAGGTGCTGGGAATGCACCATATTAAAGTCTGTGCAAAGCATACTGCCCTGTCCTTTTTGTTTC ACCTCCACCCAGACCAGCTCCTCACCTCCTCCAATAAGGCACTCAAGTTGTTGCTTATCCAAGGATATAA TCCGCTGCAAGTGGGCCACGCCATATGTTGCGTCCTGAATAAACAGATGGGGAAGCAGAATACCATTTGT TTTTACGGACCTGCTAGTACAGGGAAAACCAATTTCGCTAAAGCTATTGTACAGGGCGTGAGGCTCTATG GATGCGTTAACCACCTTAATAAAGGGTTTGTGTTCAACGATTGCAGACAACGGCTGATCATCTGGTGGGA GGAATGCCTGATGCATCAGGACTGGGTGGAGCCCGCGAAATGCATCCTCGGGGGCACAGAATGCCGGATT GACGTGAAACATAAGGATTCCGTCCTCCTGCAGCAAACCCCCGTGATAATTTCCACGAATCATGACATTT ATAGCGTCGTGGGAGGGAACACGGTCTCCCACGTGCACGCCGCCCCCTTGAAGGAAAGGGTGCTGCAACT GAATTTCATGAAGCAACTTCCACAGACTTTTGGAGAAATTAGCCCTAGTGAAATCGCAGAATTGCTCCAG TGGTGTTTCAACGAGTATGATTGCACACTTGCTGGATTCAAGCAGAAGTGGAATCTCGACAAAGTGCCTA ATTCTTTTCCCATAGGGGACTTGTGCCCTACTCATAGTCAAGATTTTACCCTCCACGAAAACGGCTTTTG TAGTGACTGTGGCGGATATCTCCCTCATAGTGCTGACGACAGTGTGTATACTGACGTGGCAAGCGAGACT ACTTCAGGAGATTACGACCCGGGTAACTTGGGGGACACTGATGGCGAGGACTCTAAGTCTGAGGCATCCG AGGTGGACTATTGCCCGCCGAAGAAGCGGCGAGTTATCAGCGCGACTCCCCCCAATTCACCAGTGTCAGG TCCCAGTTTGTCTACCTTCTTGGATACCTGGCAGTCCCAGCCACGGGACGATGACGAGCTGCGCATCTAC GAGGAGCAGGCATCCCAGTTCCAGAAGAATACTAAGAGTACAAGCGAGCGCGAAGAGGCCCAACTCGGGG AGTCCCAGGAACCGCAACCTGAGCCAGACCCGACAGCTTGGGGAGAGAAGCTGGGCGTTTGTTCCAGTCA GCAACCCGGCCAGCCTCCAATCGTGCTGTATTGCTTTGAGGACCTCCGGCCAAGTGACGAAGATGAAGGT GAAAACATCGGCGGAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100553 representing YP_002916059 Red=Cloning sites Green=Tags MAFSAPVLRAFSQPTFTYVIKFPYNNWKEDEHLLWSLLAPGTESLMIQLKNCAPHPEDDPIREDILCSLA DLHYGAVFAKACYIATSTLMGQKQRTLFPRCDIVCQSEIGSDFLHCHILVGGAGLSKRNAKISRATLLGL VMAELTQRCKLLLAHRPFEPAEATIYHELKRIEREAWSGHTGNWVQILQYKDKRGDLHAQPIDPLRFLKH YILPKNRLISPSSKPDVCTSPDNWFILADKTYSHTIINGLPLLERNRKAYLQELESEVIPGPSAMAFGGR GAWEQLPEVGEQRLITSNTSTAYKANKKEKLMLNLLDKCDELNLLVYEDLVSACPDLLLMLEGQPGGARL IEQVLGMHHIKVCAKHTALSFLFHLHPDQLLTSSNKALKLLLIQGYNPLQVGHAICCVLNKQMGKQNTIC FYGPASTGKTNFAKAIVQGVRLYGCVNHLNKGFVFNDCRQRLIIWWEECLMHQDWVEPAKCILGGTECRI DVKHKDSVLLQQTPVIISTNHDIYSVVGGNTVSHVHAAPLKERVLQLNFMKQLPQTFGEISPSEIAELLQ WCFNEYDCTLAGFKQKWNLDKVPNSFPIGDLCPTHSQDFTLHENGFCSDCGGYLPHSADDSVYTDVASET TSGDYDPGNLGDTDGEDSKSEASEVDYCPPKKRRVISATPPNSPVSGPSLSTFLDTWQSQPRDDDELRIY EEQASQFQKNTKSTSEREEAQLGESQEPQPEPDPTAWGEKLGVCSSQQPGQPPIVLYCFEDLRPSDEDEG ENIGGD TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_012729
ORF Size	2328 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_012729.2, YP_002916059
RefSeq ORF	2328 bp
Locus ID	7922604
MW	86.8 kDa

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