2A (NC_009887) Virus Tagged ORF Clone

CAT#: VC100672

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus 100], codon optimized for human cell expression, YP_001497167



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100672 represents NCBI reference of YP_001497167 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGCCTTCGGGCAGCAGAGCGGGGCAGTTTATGTTGGGAGCTACCGCATTGTGAATAGGCACCTCG
CAACCTACACAGATTGGCAGAACTGTATCTGGGAGGACTATAATAGGGATCTGCTGGTCTCCACCACCAC
AGCTCAGGGCTGTGACATTATTGCCCGCTGCCAGTGCACCACCGGAATTTACTTCTGTCAGAGCAAAAAT
AAACACTATCCAGTCTCTTTTGAAGGCCCCGGCCTTGTAGAGGTCCAGGAATCCGAGTATTACCCTAAAC
GCTACCAGAGTCATGTATTGTTGGCCGCCGGATTCAGTGAGCCAGGAGACTGCGGGGGCATCCTGAGGTG
CGAGCACGGCGTTATCGGCGTGGTTACGATGGGAGGCGAGGGAGTAGTGGGCTTCGCCGATGTCCGGGAC
CTCCTCTGGTTGGAGGACGATGCGATGGAGCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100672 representing YP_001497167
Red=Cloning sites Green=Tags

MGAFGQQSGAVYVGSYRIVNRHLATYTDWQNCIWEDYNRDLLVSTTTAQGCDIIARCQCTTGIYFCQSKN
KHYPVSFEGPGLVEVQESEYYPKRYQSHVLLAAGFSEPGDCGGILRCEHGVIGVVTMGGEGVVGFADVRD
LLWLEDDAMEQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009887
ORF Size 453 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009887.1, YP_001497167
RefSeq ORF 453 bp
MW 16.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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