VP4 (NC_014336) Virus Tagged ORF Clone

CAT＃: VC100692

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for VP4 [Human enterovirus 109], codon optimized for human cell expression, YP_003795175

Datasheet

SDS

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CNY 3,800.00

货期*

4周

规格

Product images

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100692 represents NCBI reference of YP_003795175 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGGCGCTCAAGTGTCTGCTCAGAACAACGGGACTCACGAGAACTCCAATTCAGCCAGTGGAGGGA GCACAATAAACTATACCACCATCAACTACTATAAAGATAGCGCATCCAACGCAGCAAGCAAGCTCGATTT TTCTCAAGATCCATCCAAGTTCACTGAACCTGTGAAGGATGTCATGATTAAAACCGCTCCGGCACTGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100692 representing YP_003795175 Red=Cloning sites Green=Tags MMGAQVSAQNNGTHENSNSASGGSTINYTTINYYKDSASNAASKLDFSQDPSKFTEPVKDVMIKTAPALN TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_014336
ORF Size	210 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_014336.1, YP_003795175
RefSeq ORF	210 bp
MW	7.4 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100693	Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human enterovirus 109], codon optimized for human cell expression, YP_003795176	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100694	Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human enterovirus 109], codon optimized for human cell expression, YP_003795177	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100695	Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human enterovirus 109], codon optimized for human cell expression, YP_003795178	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100696	Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus 109], codon optimized for human cell expression, YP_003795179	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100697	Myc-DDK-tagged ORF clone of viral ORF for 2B [Human enterovirus 109], codon optimized for human cell expression, YP_003795180	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100698	Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus 109], codon optimized for human cell expression, YP_003795181	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100699	Myc-DDK-tagged ORF clone of viral ORF for 3A [Human enterovirus 109], codon optimized for human cell expression, YP_003795182	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100700	Myc-DDK-tagged ORF clone of viral ORF for 3B [Human enterovirus 109], codon optimized for human cell expression, YP_003795183	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100701	Myc-DDK-tagged ORF clone of viral ORF for 3C [Human enterovirus 109], codon optimized for human cell expression, YP_003795184	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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