U90 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101624

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE1 [Human herpesvirus 7], codon optimized for human cell expression, YP_073824



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CNY 18,150.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101624 represents NCBI reference of YP_073824 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGATCTGGGGCCACCAGCCCCATGCTCATCGTTGGTCAACCAAGCGAGACTATGGCATCCAACG
ACGAGAGGCATATAGAGTACCACCCCGACACAACCGTGGAGGAGACGATTAGGGACATTCTCCAGGACTC
TTTGAAGTGCAACCCGTCTTTCGATAATACTCTGTTCCAGGAGTTGGATTCCTTTGATTTCTTGGATTCA
ATTTCTAGCAATGATATTGCCACCGCAAATGCTAGCTATAGTACTAATGTCGAGGAGGGCGCTTCCTCTG
ATATTAAATTGGCTGCCGCACAGAGCATCAGCGCCTGCATTCAGGGAATACTGAAGTCCGTTAATGCAGC
TATGGATCAAGAAAAAGCCGACATTGAGGATTGTCTCATAAACCACGCCGGCATTCTGACTAACGACCGC
CACATGCTGACTGATCTGGCCCTGGAGCAGCTCAGTCAGATGCTGAATGTGAACCTGATTAGCGGCGCAA
GCTCTGGGTTCATCAGCTTCTATGAGGATATACTGTCTGGGGAACAACTGGAATTCTTTCTGTGGTGTGA
GAGTAGATACACTGTCTATGCATGCGACAAATTCGACGAAATAGTCAAGAAAGTTATCGCCGACTGTAGG
GAATATCTGTCCGACCTCAGGAACAACATGGATAGGGAATTCATAAAGTGCATTAAGAAAATCATCTCAA
TGGGTAATTTCATTTCCTCTAATGAAAAACTGAACAGGTGTGCCACTATGCTGCTGATGATGGGAAAAAA
TAGAGACATAACTGACATCAACATAAGCAATCAGGCATTTTACATCAAGACAAATAAAATTAAAAAAGAT
CTTTTGGAGTGTAAATTCGAGATAATCGAAAAAAACAGCCGGATCGCCCAAACAATGAAAGACTTTGCTA
TCAAACAAATGAATCAAATGTTTATGTCTATGTGTGATGAAACCTTTCTGAAGATTCACTTTAATTGTAT
TAACCTGATCGAAGCCGCCAAGAATCTCGGCGCTGCCGTGTTGCAGTGCATCGTCATTTGCAGCAAGGAA
TTCAGTTGTAAAAAGTTTCGGGCTAACCGGAAGGAGTTCAAGATCACTCTCATGCACATGATCAACGATG
CTTGTGATCAGATCGAAAAGCTGTACGATGTTACCGGCGAGGTGACCGTGCTGAATAGCATCTCCATCCG
GAATGGATATGTCACAGGCAATGGTCACCACAGCTCAACCCTGCTCGAAAGCCACCTGTACAACTCAGAC
AGCACTCCGCCTGAAATCGAAGATTTCAACGACTATGGCAAATATAGCGAGGAGTGTAGCGAGACCAGTG
AGCAGAGTTACCAGTCTGTGCACCAGAACGTCTCACCGACCCAATGTGTCAGTAAATCCGAGCAGTACAA
CGTCCAGTCTAATTTCTCTGACATCTGTCAGACTATTAAAACAACTTTGGCAGTGTCACAAGCCTCCGAC
GAATCACATAGTAAGAATACGACAGGAAAAAATCCAGAAGTTTGTACTCGGGAGTATAAACTGGACTTTT
TCGAAGAGATCGACACTCAGAACCTGGCGAAGAAGGTAGTTTCCAAGGACTCCCCTGACTGCCTGATGTA
CCCAGCAGAAACAAACCCCGGCATGAGCACTGAGGAAAGCGAAGTTAATGTCAGCGAAGGAACAGATACG
CAGGACTTCTCACGCAATACGATATGCCGATTCCTGAGCGACAATCCAATTATCTCAGTCGAAAAAAATT
CAGAGGTGAATGCAATAAAGAACGAGACCGGACTTTCTGAGGCTGCCGCTACTCAGGATTTCTTCAAAGA
GGCTACTATGAAGTCCTGCCGAGATACCAAGGCGGACCAGATACTGACCGGTCAAAGGAAAACTATCAAG
AACAAGCAGAAGGTGAGCAAGAACAACGAAGCTTTTAATATGAGCCTCTACAACCTGAAGCGCAATGAGG
AAACCTATCAGGACAAGAATAGCCTGAATTTCAGCAACGAATTCCCACATACTACATTTTCCAGGAGAGA
TAAGAAGACCGACAACCAGGCCGGAATCCTGATGGAGAGGTCAGTGACCGAGGAAATTTGTGTCAATAAC
TCTCTCACTTTTTCAAATGAATTCCCCCCCACATTCTCTCGGACAGACAAAAAGACAAACTCTCCTGCAG
GAATCCTCATGGAGCGCTCAATTTTCAATAAAGATACGCAGGATAAAGAACAATACTTCGAATTGTTCAC
CATGACTAATCACACACTCGACAATCAGAATAAAGGACACAACCTCTCAGAAGACAAGACTGACGTGAAG
ATTAAGACCGACAAGTCACTTAGCGGCCAGGAGGAAATGATCAAGAATAAGCAGAGCGTGTCCCAGGGTA
ACAAGACCAGCAATGTGAATTTCCACAACTTGAACCGCAATGAAAACAAGTATAAGGACGGTAAGGATCT
GATAATTCCTGATAAGTTCCCCAATGAGACGTTCTATATTAGGGACAAGAAGAAGGATATCTCCACCAAT
AGAGCCGTGGAGAGGTCTACGGCTGATGTTGACACAGAGAGCGACGTGGAAAAAGTGGCCGCTGAGGGCC
TGATTCTGCTCGAACAAAACTCCAACAATAACTCCCGGGATACAGATAATACTAACCTCGTGATATCTGA
GATGGAGACATACGGGTACGATACCGACAAGAGCACTATCTACGAGTCCGAGTCTTCTAGCACGGACACC
AATAGTACGTTTTCATCAGTGTCCTTGACATCTTTCTCTGACAGTCAGACTACGGCCCACGATTTCTCTG
CCGTGAAAAAGAAACAGCTGAAGCGGAAGTCTGAGTCCAAGTTGAAGACTTCCAAAGCCAAGAAGAAGAA
ATTGATAGTGAAGGAAGGTTGCAATAATATGGAGCTGAATTCCTGTACAGACACCGACAACGAAAACATG
TCCAACTTCAACACCGAGATGAGCACCGATTCCATCCCATACAGGTCCAAGCTTATCAACAACAGTGACA
ATTGCGAGAACAATGGATTCCCATCTGTGGTGTTCACGATACCAATACGGTCTATTAGAGGAGTAACTCG
AGGCGTGAGAGTGAAAATAGTTCCAAAGAAAAACTGCCTTTGGTTTCTGCGAAAGACGCACAAGGTCAAC
ATTCCAGATACCGGGATGAAGAAAGATGAAAATAAGGAACTCACAAAGCAAGCGTCCTACTGCTTCAACA
ACCACTTTATCCCTATCCGCAGCAATGACGAAGAATACAGGAGAGAGAATGTGTCCTATATATTCCCCAA
GCTGCAGGAGAATTTTATCGAAGTCGACAGCATCAGCGAAACCAAGACCATGGTCACCAACATCATCATG
GATAACTTTATGGAAATCACTGAGATGATCAAAAATGGCATAAATCGACACTATGAAGAGCTTAAGAAAC
GGTACGCAAATAAAAACGTATCCGAAATGAACTCATTCATACTCGAGCAGTCCGTCGACACCCAAAATTA
TGGGAAGGAAAACACCGATTCCGATCAGACCTATGTCGATTTTTTGGAGGGCTACCCTCAGGGATATGAC
CCCTTTCTGGAAAATGCTCAGATTTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101624 representing YP_073824
Red=Cloning sites Green=Tags

MERSGATSPMLIVGQPSETMASNDERHIEYHPDTTVEETIRDILQDSLKCNPSFDNTLFQELDSFDFLDS
ISSNDIATANASYSTNVEEGASSDIKLAAAQSISACIQGILKSVNAAMDQEKADIEDCLINHAGILTNDR
HMLTDLALEQLSQMLNVNLISGASSGFISFYEDILSGEQLEFFLWCESRYTVYACDKFDEIVKKVIADCR
EYLSDLRNNMDREFIKCIKKIISMGNFISSNEKLNRCATMLLMMGKNRDITDINISNQAFYIKTNKIKKD
LLECKFEIIEKNSRIAQTMKDFAIKQMNQMFMSMCDETFLKIHFNCINLIEAAKNLGAAVLQCIVICSKE
FSCKKFRANRKEFKITLMHMINDACDQIEKLYDVTGEVTVLNSISIRNGYVTGNGHHSSTLLESHLYNSD
STPPEIEDFNDYGKYSEECSETSEQSYQSVHQNVSPTQCVSKSEQYNVQSNFSDICQTIKTTLAVSQASD
ESHSKNTTGKNPEVCTREYKLDFFEEIDTQNLAKKVVSKDSPDCLMYPAETNPGMSTEESEVNVSEGTDT
QDFSRNTICRFLSDNPIISVEKNSEVNAIKNETGLSEAAATQDFFKEATMKSCRDTKADQILTGQRKTIK
NKQKVSKNNEAFNMSLYNLKRNEETYQDKNSLNFSNEFPHTTFSRRDKKTDNQAGILMERSVTEEICVNN
SLTFSNEFPPTFSRTDKKTNSPAGILMERSIFNKDTQDKEQYFELFTMTNHTLDNQNKGHNLSEDKTDVK
IKTDKSLSGQEEMIKNKQSVSQGNKTSNVNFHNLNRNENKYKDGKDLIIPDKFPNETFYIRDKKKDISTN
RAVERSTADVDTESDVEKVAAEGLILLEQNSNNNSRDTDNTNLVISEMETYGYDTDKSTIYESESSSTDT
NSTFSSVSLTSFSDSQTTAHDFSAVKKKQLKRKSESKLKTSKAKKKKLIVKEGCNNMELNSCTDTDNENM
SNFNTEMSTDSIPYRSKLINNSDNCENNGFPSVVFTIPIRSIRGVTRGVRVKIVPKKNCLWFLRKTHKVN
IPDTGMKKDENKELTKQASYCFNNHFIPIRSNDEEYRRENVSYIFPKLQENFIEVDSISETKTMVTNIIM
DNFMEITEMIKNGINRHYEELKKRYANKNVSEMNSFILEQSVDTQNYGKENTDSDQTYVDFLEGYPQGYD
PFLENAQIY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 3597 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073824
RefSeq ORF 3597 bp
Locus ID 3289542
MW 136.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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