vpr (NC_001802) Virus Tagged ORF Clone

CAT＃: VC101720

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for Vpr [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057852

Datasheet

SDS

View other clones from "Virus" (19)

Need custom modification / cloning service?
Get a free quote

CNY 1,200.00

CNY 3,800.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101720 represents NCBI reference of NP_057852 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACAGGCTCCAGAAGACCAAGGGCCTCAGAGAGAGCCTCACAACGAGTGGACACTGGAGCTGCTGG AGGAACTGAAGAACGAGGCTGTGAGGCATTTCCCCCGCATATGGTTGCATGGCCTTGGGCAGCACATTTA CGAAACATACGGAGATACGTGGGCTGGTGTGGAGGCAATCATCCGCATTCTGCAGCAGCTGCTGTTCATC CACTTCCGGATCGGCTGTCGACACTCCCGCATCGGGGTGACTCGGCAACGAAGGGCGAGAAATGGCGCGT CAAGGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101720 representing NP_057852 Red=Cloning sites Green=Tags MEQAPEDQGPQREPHNEWTLELLEELKNEAVRHFPRIWLHGLGQHIYETYGDTWAGVEAIIRILQQLLFI HFRIGCRHSRIGVTRQRRARNGASRS TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_001802
ORF Size	288 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001802.1, NP_057852
RefSeq ORF	288 bp
Locus ID	155807
UniProt ID	P04585
MW	11.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC101716	Myc-DDK-tagged ORF clone of viral ORF for Tat [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057853	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC101717	Myc-DDK-tagged ORF clone of viral ORF for Rev [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057854	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC101718	Myc-DDK-tagged ORF clone of viral ORF for Pr55(Gag) [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057850	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 6,080.00
VC101719	Myc-DDK-tagged ORF clone of viral ORF for Vif [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057851	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC101721	Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp160, precursor [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057856	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,272.00
VC101722	Myc-DDK-tagged ORF clone of viral ORF for Nef [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057857	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC101723	Myc-DDK-tagged ORF clone of viral ORF for Vpu [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057855	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC101724	Myc-DDK-tagged ORF clone of viral ORF for matrix [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579876	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,800.00
VC101725	Myc-DDK-tagged ORF clone of viral ORF for capsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579880	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00
VC101726	Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579881	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,800.00
VC101727	Myc-DDK-tagged ORF clone of viral ORF for p2 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579882	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC101728	Myc-DDK-tagged ORF clone of viral ORF for p6 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579883	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,800.00
VC101729	Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579893	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC101730	Myc-DDK-tagged ORF clone of viral ORF for Envelope transmembrane glycoprotein gp41 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579895	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 4,090.00
VC101732	Myc-DDK-tagged ORF clone of viral ORF for retropepsin [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_705926	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,800.00
VC101735	Myc-DDK-tagged ORF clone of viral ORF for p1 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787042	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC101736	Myc-DDK-tagged ORF clone of viral ORF for Gag-Pol Transframe peptide [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787043	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC101737	Myc-DDK-tagged ORF clone of viral ORF for reverse transcriptase p51 subunit [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_789739	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 5,420.00
VC101739	Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp120 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579894	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 5,890.00

其它vpr产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies