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SH (NC_004148) Virus Tagged ORF Clone
CAT＃: VC101759
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for small hydrophobic protein [Human metapneumovirus], codon optimized for human cell expression, YP_012611
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CNY 3,800.00
| Cited in 2 publications. |
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101759 represents NCBI reference of YP_012611 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTACCTTGGATGTGATAAAAAGCGACGGTAGCTCAAAGACTTGCACTCATTTGAAGAAAATCATTA AAGACCATTCCGGGAAGGTGCTCATCGCCTTGAAACTGATCCTTGCATTGTTGACCTTCTTCACCATTAC TATCACAATTAACTACATCAAGGTGGAGAACAACCTGCAGATCTGCCAGTCCAAAACCGAAAGCGATAAA GAGGACAGCCCGTCTAACACCACATCAGTTACAACCAAGACTACCCTGGACCATGATATCACCCAATATT TCAAAAGATTGATTCAGAGGTACACTGACAGCGTGATAAATAAGGATACTTGCTGGAAAATCAGCCGGAA TCAGTGTACTAATATTACAACATACAAATTCTTGTGTTTCAAGCCCGAAGACTCTAAAATTAACTCTTGC GATAGGCTCACGGATTTGTGCCGGAATAAATCCAAAAGCGCGGCCGAAGCCTATCACACCGTCGAGTGCC ACTGCATATATACCATTGAATGGAAGTGTTACCACCACTCCATTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101759 representing YP_012611
Red=Cloning sites Green=Tags MITLDVIKSDGSSKTCTHLKKIIKDHSGKVLIALKLILALLTFFTITITINYIKVENNLQICQSKTESDK EDSPSNTTSVTTKTTLDHDITQYFKRLIQRYTDSVINKDTCWKISRNQCTNITTYKFLCFKPEDSKINSC DRLTDLCRNKSKSAAEAYHTVECHCIYTIEWKCYHHSID TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_004148 |
| ORF Size | 537 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_004148.2, YP_012611 |
| RefSeq ORF | 537 bp |
| Locus ID | 2799941 |
| MW | 20.6 kDa |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulation of microRNA miR-197-3p/CDC28 protein kinase regulatory subunit 1B (CKS1B) axis by Circular RNA hsa_circ_0000285 promotes glioma progression
,null,
Bioengineered
,PubMed ID 35174774
[SH]
|
|
Increased expression of BCL11B and its recruited chromatin remodeling factors during highly active antiretroviral therapy synergistically represses the transcription of human immunodeficiency virus type 1 and is associated with residual immune activation.
,null,
Archives of virology
,PubMed ID 31828511
[SH]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101753 | Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Human metapneumovirus], codon optimized for human cell expression, YP_012605 |
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|
| VC101754 | Myc-DDK-tagged ORF clone of viral ORF for phosphoprotein [Human metapneumovirus], codon optimized for human cell expression, YP_012606 |
CNY 3,800.00 |
|
| VC101755 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein [Human metapneumovirus], codon optimized for human cell expression, YP_012607 |
CNY 3,800.00 |
|
| VC101756 | Myc-DDK-tagged ORF clone of viral ORF for fusion glycoprotein precursor [Human metapneumovirus], codon optimized for human cell expression, YP_012608 |
CNY 6,560.00 |
|
| VC101757 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein M2-1 [Human metapneumovirus], codon optimized for human cell expression, YP_012609 |
CNY 3,800.00 |
|
| VC101758 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein M2-2 [Human metapneumovirus], codon optimized for human cell expression, YP_012610 |
CNY 3,800.00 |
|
| VC101760 | Myc-DDK-tagged ORF clone of viral ORF for attachment glycoprotein G [Human metapneumovirus], codon optimized for human cell expression, YP_012612 |
CNY 3,800.00 |
