E1 (NC_008189) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101770 represents NCBI reference of YP_656500 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATAAGGATAACAAGGGCACCTCCCCTCACATTTGTGACTATAATGCCATTGACTTTATTGATG CCGAGGCCGCCTGCGAAGAACTCGATCCTTTGGAGGCCGTCCTGGAAGAGTCAGACGGCACCGATATAAG TGACCTGATTGACAATACAGAAGAACCAGACGAGGGCAATAGTCTGGCTTTGTTGAATAAGCAGCAGCTC GAGGATGATACCCAACAGCTGAAGATTCTTAAACGCAAATATTTCTCTCCCAGCCCCAAGCAGCAGGCAG TGATTGATCTCTCACCCCGCCTGCAGCAGTTGCGGGTCTCTAGTCAAGGGTCTTCCAAGAGGAGGCTGGT ATTCGAGGATAGCGGACTCGGTAACGAGACTGAGGATTCCGTCACTGAAGTGGCCAAGGTTGCCTCTGAG CAGCCACTGAGCTGTAACCCCGGCGAACCAGAGACAAACGAAGACGTGAGTACGGACGTAGAGACTCGGT CAGGAGGCCCTTGCGGCGGCACAACAACCAGTAACAATAACTCCAACAACAACTGCATCGACCTGCTCCA TTCATCAAACAGGGAAGCCACTGCCTACGCCAAATTCAAGGCCACCTTTGATGTGGGATATAAGGAGCTG ACACGACCGTTCATATCCAACAAATCCTGCTGCTGTTCCTGGATCGCTGGTATCTTTGGCGTCGTTGCCG AAATACTCGAGGCGGCCAAGACACTGCTGCAGCCACATTGCGAATATCTGCAAATAATCAATCCTTCCAT GGGAACAGGGGTCACCGTGATGATGCTCTTCCAGTTCTACGCAGCCAAGTGTCGGGATACTGTGATCAAT CTGCTGTGCAACGTTCTTCATGTCAGAGAGTGGCAGATCATCACCAATCCACCCAAACACCGCAGTGTGG CAGTCGCTCTGTACTTCTACAAGACGAGTATGTCTAACGTCAGCTATTGCTACGGCGCAATGCCAGAGTG GATCAAAAAACAAACGCTTGTGAATCATCAGCAAGAAAGCGATAATTTTGAGTTTGCAACCATGGTGCAG TGGGCCTATGATAATAACATCCGCGATGAAGCAGAGGTTGCATACGGTTACGCCTCACTGGCCGATGATG ACACTAACGCTGCCGCCTGGCTGAAGTGTAACAATCAATTTAAGTACGTGAAAGATTGTGTGCAAATGGT TGCTATGTACAAGAGGTACGAGATGCGGAACATGACAATCGGGCAGTGGCTCGTGAAATGCGGCGAGCGA GTTACTGAGGAAGGAAATTGGAAAAACATCATCAACTTCTTGAAATACCAAGAGATTTCCATCGTGGCAT TTCTCACTACTCTCAGATATTTCTTGCAGGGGAGACCGAAGAAAAACTGTCTCGCTATCTGGGGGCCACC TGACACCGGTAAATCTATGTTCTGTTATTCCCTGATTAAATATACTCAGGGCAAGGTCGTTTCTTTTGTA AACAGCCGGTCCCAGTTCTGGCTGCAGCCCCTGGTGGACGGAAAAATTGGACTCATTGATGACGCTACAT TCGCTTGCTATCAGTATATGGATGTATATATGCGGAACGGTTTGGATGGCAACGCCGTGAGTGTCGACGT CAAACACAAGGCTCCCATCCAGCTGAAACTGCCTCCCCTTCTGCTTACATCTAATATCGAAGTCCACGCC GAGGCCCCGCTGAAGTACCTCCACAGTCGCATACAGGAGTATAAGTTCCCAAACAAACTCAGGCTGGATG CAAACGGGAATCCGATCATTACGATAACCGACGCTGATTGGAAAAGTTTCTTCTCAAAGCTGTGGAAGCA ACTGGACCTCGGAGATCCTGATGACAGTTCTGACGGCGATCCGGGCAGGCCCTTCCGGTGTTGCGCACGG CAGGCCACTGGAGATCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101770 representing YP_656500 Red=Cloning sites Green=Tags MEDKDNKGTSPHICDYNAIDFIDAEAACEELDPLEAVLEESDGTDISDLIDNTEEPDEGNSLALLNKQQL EDDTQQLKILKRKYFSPSPKQQAVIDLSPRLQQLRVSSQGSSKRRLVFEDSGLGNETEDSVTEVAKVASE QPLSCNPGEPETNEDVSTDVETRSGGPCGGTTTSNNNSNNNCIDLLHSSNREATAYAKFKATFDVGYKEL TRPFISNKSCCCSWIAGIFGVVAEILEAAKTLLQPHCEYLQIINPSMGTGVTVMMLFQFYAAKCRDTVIN LLCNVLHVREWQIITNPPKHRSVAVALYFYKTSMSNVSYCYGAMPEWIKKQTLVNHQQESDNFEFATMVQ WAYDNNIRDEAEVAYGYASLADDDTNAAAWLKCNNQFKYVKDCVQMVAMYKRYEMRNMTIGQWLVKCGER VTEEGNWKNIINFLKYQEISIVAFLTTLRYFLQGRPKKNCLAIWGPPDTGKSMFCYSLIKYTQGKVVSFV NSRSQFWLQPLVDGKIGLIDDATFACYQYMDVYMRNGLDGNAVSVDVKHKAPIQLKLPPLLLTSNIEVHA EAPLKYLHSRIQEYKFPNKLRLDANGNPIITITDADWKSFFSKLWKQLDLGDPDDSSDGDPGRPFRCCAR QATGDL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_008189
ORF Size	1908 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_008189.1, YP_656500
RefSeq ORF	1908 bp
Locus ID	5177020
MW	71.7 kDa

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