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E7 (NC_001352) Virus Tagged ORF Clone
CAT＃: VC101812
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for early protein E7 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077117
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CNY 3,800.00
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CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101812 represents NCBI reference of NP_077117 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGGTAACCGACCCTCCTTGAAAGATATCACCCTCATTCTCGACGAGATTCCCGAAATTGTCGACC TTCACTGTGACGAACAATTCGACTCCAGCGAAGAGGAAAATAATCACCAGCTGACTGAACCCGATGTGCA GGCCTATGGAGTGGTGACCACTTGTTGTAAATGCGGCAGAACCGTGAGACTGGTGGTGGAATGCGGCCAG GCCGATCTGAGAGAGTTGGAGCAATTGTTCCTCAAAACCCTGACGCTCGTCTGCCCACACTGCGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101812 representing NP_077117
Red=Cloning sites Green=Tags MHGNRPSLKDITLILDEIPEIVDLHCDEQFDSSEEENNHQLTEPDVQAYGVVTTCCKCGRTVRLVVECGQ ADLRELEQLFLKTLTLVCPHCA TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001352 |
| ORF Size | 276 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001352.1, NP_077117 |
| RefSeq ORF | 276 bp |
| Locus ID | 1489226 |
| MW | 10.4 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101811 | Myc-DDK-tagged ORF clone of viral ORF for early protein E6 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077116 |
CNY 3,800.00 |
|
| VC101813 | Myc-DDK-tagged ORF clone of viral ORF for early protein E1 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077118 |
CNY 7,700.00 |
|
| VC101814 | Myc-DDK-tagged ORF clone of viral ORF for early protein E2 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077119 |
CNY 4,660.00 |
|
| VC101815 | Myc-DDK-tagged ORF clone of viral ORF for early protein E4 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077120 |
CNY 3,800.00 |
|
| VC101816 | Myc-DDK-tagged ORF clone of viral ORF for late protein L2 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077121 |
CNY 6,370.00 |
|
| VC101817 | Myc-DDK-tagged ORF clone of viral ORF for late protein L1 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077122 |
CNY 6,180.00 |
