E1 (NC_001576) Virus Tagged ORF Clone

CAT#: VC101834

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041743



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CNY 10,260.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101834 represents NCBI reference of NP_041743 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGATAATACCGGGACCGAAGGCGGAGCCTGTTCAGAGTCAGAAAGGGCAGGAGGCTGGTTTATCG
TGGAGGCTATTGTGGATAGAAGGACTGGCGATCCCATCAGTTCTGATGACGACGAGGAGGAGGACGAGGC
TGGAGAGGACTTCGTGGACTTCATCGATGACACCAGGTCCCTGGGAGACGGCCAAGAGGTGGCACAAGAG
CTCTTCCAGCAGCAAACCGCTGCAGATGACGATGTGGCAGTGCAGACAGTGAAGAGGAAGTTTGCCCCAT
CCCCATACTTCAGCCCCGTGTGCGAACAGGCGAGCATCGAACATGAGCTGTCTCCCCGGCTGGATGCTAT
TAAACTCGGGCGCCAATCTGCGAAGGCGAAACGACGGCTCTTCGAGCTGCCCGATAGCGGGTATGGTCAG
ACACAGGTAGACACTGAATCCGGACCTAAGCAAGTACAGGGCTCTAGTGAAACACAGGATGGCAGACAGG
ATGATGATGAGGGGTCTGTAGTTCAGTCTACCCTCGACACAGGGAACCAGAACGGTCGGCAAAACAACGA
CGAGGGATCTGGACGCAATGTGGGTGAACATGGTAGCCAGGAAGAGGAGAGGGCCGGGGGCGACGGAGAA
GAGAGTGACCTGCAGTCTACATCCACAGGGAAGGGTGCAGGAGGAGTAGTTGAGATTCTGAGGGCAAGCA
ATAAAAAGGCAACACTTCTTGGCAAATTCAAAGAACAGTTCGGCCTGGGCTACAACGAACTTATTCGGCA
TTTTAAAAGCGACCGAACGTCATGCGCCGACTGGGTGGTCTGTGTTTTCGGCGTCTTCTGCACAGTCGCT
GAAGGCATCAAGACTCTCATTCAGCCTCTTTGCGACTACGCTCATATCCAGGTGCTGCCCTGCCAGTGGG
GCATGACAGTGTTGATGCTGGTTAGGTACAAGCGGGCCAAAAACAGAGAAACAGTGGCAAAAGGACTGAG
TACACTTCTGAATGTGCCGGAGAGCCAAATGCTTATTGAGCCTCCCAAATTGCGGTCTGGGCCAGCCGCC
CTGTATTGGTATAAAACTTCTATGTCATCCTGTTCAGACGTGTACGGCGAGACCCCCGAGTGGATTGTGA
GGCAGACTATGGTCGGTCACGCCATGGAGGATGCGCAGTTTAGCCTGTCAGAAATGGTCCAGTGGGCATA
CGACCACGACATTACAGATGAAAGCACCCTGGCATACGAGTATGCTCTGATCGCCGATACAGATTCTAAT
GCCGCCGCTTTCTTGTCATCAAATTGCCAGGCCAAATATCTGAAGGATGCCTGTACAATGTGTAGACATT
ACAAACGGGGCGAGCAGGCCCGCATGAGCATGTCTGAGTGGATCTGGTTTAGAGGCGACAAAGTACAGGG
CGATGGAGATTGGAAACCCATAGTTCAATTTTTGCGATATCAGGATGTTGAATTCATACCTTTTCTGTGC
GCCTTCAAAACCTTTCTGCAGGGAGTGCCCAAGAAGAGCTGCTTGGTGTTCTATGGGCCTGCCGACACTG
GCAAGAGCTATTTCTGCATGAGTCTGCTGCGGTTTCTCGGAGGGGCAGTTATTAGCTATGCCAATTCTTC
CTCCCATTTTTGGCTGCAGCCACTCAGCGAGGCCAAAATTGGTTTGTTGGACGATGCTACCTCTCAGTGC
TGGAATTACATCGACACATACCTCCGAAACGCTCTCGACGGGAACCAGATTTGTGTGGACAGAAAACATC
GAGCCTTGCTGCAGTTGAAATGTCCTCCTCTGCTTATCACTACTAACATCAACCCTCTCACCGATGAAAG
ATGGAAATTCCTGAGGTCTCGCTTGCAACTTTTCACATTCAAGAATCCATTCCCAGTTACAACTCAGGGC
GAACCTATGTATACCCTGAATGACCAGAACTGGAAGTGCTTCTTCAGGCGGCTGTGGGCCAGGCTGAGTC
TCACCGATCCCGAAGACGAGGAGGAGCACGGCAATCCCTCCGAGCCTTTCCGCTGCGTGCCCGGACAGAA
TGCTAGGACCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101834 representing NP_041743
Red=Cloning sites Green=Tags

MDDNTGTEGGACSESERAGGWFIVEAIVDRRTGDPISSDDDEEEDEAGEDFVDFIDDTRSLGDGQEVAQE
LFQQQTAADDDVAVQTVKRKFAPSPYFSPVCEQASIEHELSPRLDAIKLGRQSAKAKRRLFELPDSGYGQ
TQVDTESGPKQVQGSSETQDGRQDDDEGSVVQSTLDTGNQNGRQNNDEGSGRNVGEHGSQEEERAGGDGE
ESDLQSTSTGKGAGGVVEILRASNKKATLLGKFKEQFGLGYNELIRHFKSDRTSCADWVVCVFGVFCTVA
EGIKTLIQPLCDYAHIQVLPCQWGMTVLMLVRYKRAKNRETVAKGLSTLLNVPESQMLIEPPKLRSGPAA
LYWYKTSMSSCSDVYGETPEWIVRQTMVGHAMEDAQFSLSEMVQWAYDHDITDESTLAYEYALIADTDSN
AAAFLSSNCQAKYLKDACTMCRHYKRGEQARMSMSEWIWFRGDKVQGDGDWKPIVQFLRYQDVEFIPFLC
AFKTFLQGVPKKSCLVFYGPADTGKSYFCMSLLRFLGGAVISYANSSSHFWLQPLSEAKIGLLDDATSQC
WNYIDTYLRNALDGNQICVDRKHRALLQLKCPPLLITTNINPLTDERWKFLRSRLQLFTFKNPFPVTTQG
EPMYTLNDQNWKCFFRRLWARLSLTDPEDEEEHGNPSEPFRCVPGQNARTI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001576
ORF Size 2043 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001576.1, NP_041743
RefSeq ORF 2043 bp
Locus ID 1489373
MW 76.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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