E1 (NC_008188) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101840 represents NCBI reference of YP_656494 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGATAAGGATACTAAAGGGACATCCTGCTTTACCGACATTACCGCCAACGCTTGCGAATTTATTG AAACTGAGGCTACCTGTGAGGACGGCCTGGACTCCCTGGAGGCTCTGTTTGAGGAATCTGATGGTACTGA TATCAGCGAGCTGATAGAGAACATTGATGAGCCTGATCGCGGCAATCCTCAAGCACTCTTGAACCGCCAG CAGCTGGAGGAAGACAGTCAGTTGCTGACCGTTCTCAAGCGCAAGTACGTTTCCCCTTCTCCGAAACAAC GGGTGATAGATCTGTCACCTCGACTGGAAGCTCTGTGTGTGTCTCCGAGAGGATCCTCCTCAAAACGGCG CCTGTTCGAAGACAGCGGTCTCGGACATGAGCCTGAAGATTCCAATGCCGAAGTTGAAAAAGTGGCCTCT GAGGCTCTCACCAACAGCGGGACTCTGACTAATGGCGATGAGAACGCTGTGTGTGCTACTCAGGACGGAG ACACACGCTGCCTGGACATCCTTCATTCTTCCAATCGCCAAGCGACGGCACTTACCAAATTCAAGAATGT CTTTGGGGTAGGGTACAAAGAAATTACGCGACCATTCCAATCCAACAAAAGCTGCTGTCACTCCTGGGTA GCAGCGATCTTCGGAGTAGTGGCTGAGATGCTCGAAGCCGCAAAGACCCTGTTCAAGGTTCATTGCGATT ATCTTCAGATTATCAACCCAAGTATGGGCACAGGCGTCACCGTGCTGTGCTTGTTTGAGTTCTCCAGTAG TAAGTGTCGCGATACAGTGCAGAAGCTTATCTCTAATGTGCTCAACGTGCAGGAACATCAGATCATTACC GACCCCCCCAGGCACCGGAGCGTGCCCGTTGCGCTGTTTTTCTATAAGCAGAGCATGTCCAATACCAGCT TTGTGCACGGAACCATGCCTGACTGGCTGAAACGGCAAACCATGCTGAACCACCAGCAAGAGGCCGACAG CTTTGAGTTGTCTCATATGGTGCAGTGGGCCTACGACAACAATATTTGGGACGAGGCAGAGCTGGCCTAT CAGTACGCCTGTCTCGCTGATGTGGAGCCCAACGCCGCCGCCTGGCTTAAATCTAACCAACAATACAAGT ACGTCAGTGATTGCGCCAAGATGGTGCGAATGTATAAAAAGTATGAAATGCAGCAAATGTCCATGGCACA GTGGATTAAAAAGTGTGGAGAGCACATTGAGACAGACGGCGATTGGAAGAAGATCATCAACCTGCTTAAG TACCAAGAGATTTCAGTGATTGCCTTTCTGACCAGTTTCCGCATGTTCCTGAAAGGGCAGCCAAAAAAGA ACTGCATAGCCCTGTGGGGACCACCTGATACCGGAAAAAGTATGTTCTGCTATAGCCTGATCAGGTACGT GAAAGGCAAAGTCGTTTCTTACGCTAACAGCAAATCCCAATTCTGGCTGCAGCCCTTGACCGATGCTAAA CTCGGTCTCATAGATGACGCCACTTTTCCATGTTTTCAATTTATGGACGTCTACATGCGATCTGCACTTG ATGGGAACGAAGTTTCTGTGGACTGTAAACACCGGGTACCTGTGCAAATTAAGCTGCCGCCACTGCTGGT GACAAGTAATATTGACATGCATAGTGAGGCCTCCCTGAAATACCTTCAAAGCCGGATTACCAGCTTTAAG TTCCCACACAAACTCCCGCTGGATACTAACGGGAATCCCATCTTCATTATCACCGATACTGACTGGAAGT GCTTCTTCTCCAAGCTCGAACAGCAGCTTGATCTTTGCGATCCAGAAGATAGTACGAACGGAGACCCAGG AGGAGCCTTCAGATGTTGCCCCAGGGAGACAACAGGCGCTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101840 representing YP_656494 Red=Cloning sites Green=Tags MEDKDTKGTSCFTDITANACEFIETEATCEDGLDSLEALFEESDGTDISELIENIDEPDRGNPQALLNRQ QLEEDSQLLTVLKRKYVSPSPKQRVIDLSPRLEALCVSPRGSSSKRRLFEDSGLGHEPEDSNAEVEKVAS EALTNSGTLTNGDENAVCATQDGDTRCLDILHSSNRQATALTKFKNVFGVGYKEITRPFQSNKSCCHSWV AAIFGVVAEMLEAAKTLFKVHCDYLQIINPSMGTGVTVLCLFEFSSSKCRDTVQKLISNVLNVQEHQIIT DPPRHRSVPVALFFYKQSMSNTSFVHGTMPDWLKRQTMLNHQQEADSFELSHMVQWAYDNNIWDEAELAY QYACLADVEPNAAAWLKSNQQYKYVSDCAKMVRMYKKYEMQQMSMAQWIKKCGEHIETDGDWKKIINLLK YQEISVIAFLTSFRMFLKGQPKKNCIALWGPPDTGKSMFCYSLIRYVKGKVVSYANSKSQFWLQPLTDAK LGLIDDATFPCFQFMDVYMRSALDGNEVSVDCKHRVPVQIKLPPLLVTSNIDMHSEASLKYLQSRITSFK FPHKLPLDTNGNPIFIITDTDWKCFFSKLEQQLDLCDPEDSTNGDPGGAFRCCPRETTGAL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_008188
ORF Size	1863 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_008188.1, YP_656494
RefSeq ORF	1863 bp
Locus ID	5177014
MW	70.1 kDa

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