L1 (NC_001690) Virus Tagged ORF Clone

CAT#: VC101949

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043422



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CNY 6,270.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101949 represents NCBI reference of NP_043422 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGTGGTCCGCCGTCCCTGGTAAGGTCTACCTTCCACCATCTGCACCTGTGGCAAGAGTGCTTA
GAACTGACGAGTACGTACAGGAAACCGACGTATACTTCTATACTTCCACTGAGCGCCTTTTGATCGTGGG
CAATCCCTACTTCGATGTTGAAAACCGGGATACAATCACTGTACCGAAGGTGTCAGCTAATCAGTATCGG
GTATTCCGCTGTAAGCTCCCTGACCCAAACAAATTCGCGTTGGTGGACAAAAACCTTTACAACTCTGATA
AGGAGAGACTCGTGTGGAAACTGGTTGGACTCGAAGTGGGTAGGGGCGGCCCACTCGGGGTGGGCTCCAC
CGGACATCCGCTGCTTAATAAAATAGGAGATACAGAAAATCCTAGCTTTTACTTGGGAGAGCAGACTAAG
GATGAGCGCCAGAACGTCAGTATGGACCCTAAACAGAGCCAGATCCTTATCGTGGGGTGTGCCCCAGCAA
CGGGCGAGTATTGGGACCTGGCCAAGCCCTGTAATGACCTGGAGAACGGCGCCGCCCCTCCCATCCAGCT
GGTCAATACGGTTATCCAGGACGGCGACATGGGGGATATCGGTTTCGGTGCGGCGAACTTCCCAAAGTTG
ATGCAGGACCGCGCTGGCGTCCCACTTGAGCTGATCGACTCTATATCCATCTGGCCGGATTTCTTGAAGA
TGACTAAGGATATCTACGGGGACTCCGTATTTTTTTTCGGCAAAAGAGAGCAGTGCTACGCACGACATCT
GTTCGCTCGGGCAGGGCAGATGGGCGAGCCAATTCCCACAGAGAACGGCGTCTATTATATCACACCCGAT
TCCGCCGACCAAAACAACCGAAGTTCCCACCTTGGTTCCAGTGTGTATTTTACCACCCCTTCTGGCAGCT
TGAATACTTCAGACTCACAACTGTTCAACCGACCGTATTGGCTTCGGCGAGCCCAGGGTACCAACAACGG
AATCTGCTGGGGCAACGAGCTGTTTATTACTGTGTTTGACAATACCCACAATGTCAACTTTACAATCAGC
GTCAAGAACGACAAGACCGCGCTTACTGAGAACTATATAGACAACGGGTACAAGTATAATAATGCCGATT
TTAAGCAGTACCTCCGGCACACGGAGGAGTACGAGATAGAGCTCGTGTTTCAGTTGTGCAAAGTGAACCT
GACTGCCGACGTCCTGGCCCATCTGCACGTGATGAACCCGCGCATACTGGAGGAGTGGCAGTTGGCGTTT
GTGCCTCCAGCTCCTACCGGCATCGAGGATACCTATCGGTATATCAAGTCCATGGCAACTAAATGTCCCA
CTGCTGAGCCAGAGGAAGACACTGACCCGTATAAAGCCTATTCTTTCTGGACACTCGATATGACAGAGCG
GTTTAGCTCCGACCTCTCACAGTTTAGCCTGGGGCGGAAATTCCTCTACCAGACTGGCCTCCTCAACGGA
AAAAGAGCCCGCACTGACTATACAGCCGCAGGGTCTTCCACTAGATCTACCAAGCGGAGGCGCGTTCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101949 representing NP_043422
Red=Cloning sites Green=Tags

MALWSAVPGKVYLPPSAPVARVLRTDEYVQETDVYFYTSTERLLIVGNPYFDVENRDTITVPKVSANQYR
VFRCKLPDPNKFALVDKNLYNSDKERLVWKLVGLEVGRGGPLGVGSTGHPLLNKIGDTENPSFYLGEQTK
DERQNVSMDPKQSQILIVGCAPATGEYWDLAKPCNDLENGAAPPIQLVNTVIQDGDMGDIGFGAANFPKL
MQDRAGVPLELIDSISIWPDFLKMTKDIYGDSVFFFGKREQCYARHLFARAGQMGEPIPTENGVYYITPD
SADQNNRSSHLGSSVYFTTPSGSLNTSDSQLFNRPYWLRRAQGTNNGICWGNELFITVFDNTHNVNFTIS
VKNDKTALTENYIDNGYKYNNADFKQYLRHTEEYEIELVFQLCKVNLTADVLAHLHVMNPRILEEWQLAF
VPPAPTGIEDTYRYIKSMATKCPTAEPEEDTDPYKAYSFWTLDMTERFSSDLSQFSLGRKFLYQTGLLNG
KRARTDYTAAGSSTRSTKRRRVR

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001690
ORF Size 1539 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001690.1, NP_043422
RefSeq ORF 1539 bp
Locus ID 1403627
MW 58.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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