United States
Germany
Japan
United Kingdom
China
E1 (NC_001691) Virus Tagged ORF Clone
CAT＃: VC101966
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043425
View other clones from "Virus" (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,220.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101966 represents NCBI reference of NP_043425 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAACTCAAGGGCACCGATAACAACTCCAACGTCGAGATCATTAATGAACTTTTTGACAACGAAG CAGTGTGTGATGATGATTCTTTCCAGGAGCTCTTTGATGAAAGCACTGACGAGTCCACTATTAGTAATCT TATCGACGACTCCGAGAACGTCGTGCAGGGCAACTCTCATGCCCTTCTGAATGCCCAACTGAGTGAGGAG TACGATAAAGACCTGGTCACAGTGAAAAGGAAGTTTTACGCCACACCAGAAAAACTGGCATCAGACCTTT CCCCGCGACTCAGTGCCGTGCACATCACGCCCGAAAGGCAGTCTAAGCGACGGCTCTTTCGCGATTCAGG GATTGAGGACGAGGCCGAAAATTCTATCGTGCAGGTACAGGACGACAGTTCAAACGTGGCAGGAAATAAG AATGGTGCCGATGCTGAACTGTACTCACTGCTCCACAGTAATAATAGACGGGCCGCTCTTCTGTGTAAAT TCAAGGAGAAATACGGAATCCCCTTTAACGAAATAACTCGGACTTTTAAGTCTAACAAATCTTGTACCCA AAATTGGATCATTGTGGTCTTCGCCTGCGCTGAAGATCTGATTGAGGCAAGCAAGACAACTATGCAGAAT CACGTGTCATACCTGCAGATGATTACTTCTGACTTTAGCGCCCTTTATATCATTTGCTTTAAAGCTGCCA AATCCCGGGAGACCGTGGTCAAGTTGATTAACTCTCTCCTGAACACGAAGGAGGAGCAGGTGCTTTGCGA TCCTCCAAAAATCAAGTCCATGGCAGCCGCTCTTTACTGCTACAAGAAAGTTATTGCAGATACTTGCTAC AAGTATGGCGATTTTCCAGACTGGATTGCAACCCATACTGTGATCAATCATCAGCTGGCGACTGCCGATA GCTTTAAATTCTCAGACATGGTGCAGTGGGCCTACGATAACGACATGCTTGACGAGGCAGCTATTGCTTA CAACTACGCTTGTTATGCCAGTGAAAACGAAAACGCTGCCGCCTTTCTCCAGACCAATTCACAGCTTAAG TACGTGAAAGAATGCTGCGCTATGGTGCGCCTGTACAAGAAGCAAGAAATGCGAAATATGACTATGCCAG AGTGGATAAAATCCTGTTTCACAAACAATTACAATTCCGATGACTGGAAGGTCATTGTCCGCTATCTGAA ATATCAAAATATTAATTTCCTCGAGTTTCTCCTGGCCCTGAAACTGCTCCTGAAGGGAATCCCCAAGAAA ATGTGCCTTGTCATATATGGCCCCCCCGATACTGGAAAATCTTATTTCTGCTATCAATTTATCCAATTCA TGAGAGGTAAGGTGGTGTCTTTCATGAATAAGAATAGCCACTTCTGGCTGATGCCCCTGCTGGACTCCAA AATTGGCTTTCTGGATGACGCCACACAGTGTTGCTGGATGTACCTTGATACCCACATGCGCAATGCCTTT GATGGTAACGCGGTGTCTGTGGACGTTAAACACAAGAATCTGCAGCAGATCGTCTTGCCACCCATGCTCA TCACTACAAATTGTGACGTATGTAGGGATCCAACATTTATGTACCTTCGGAGCCGCCTGACCTGCTTCAA CTTCCCCAATAAGCTGCCACTTTATGAGAACGGCGAGCCTAAGTTTAAGTTCACTGATAACTGTTGGACC TCTTTTTTTTCCAAGTTCTGGAAACATCTGGATCTCCCGGAAGACGATCCGGATGGTGACACCGGGAACT CTGAGCGGACATTCTCCTGCACCACTCGGCACAGTATCGAAAGCGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101966 representing NP_043425
Red=Cloning sites Green=Tags MAELKGTDNNSNVEIINELFDNEAVCDDDSFQELFDESTDESTISNLIDDSENVVQGNSHALLNAQLSEE YDKDLVTVKRKFYATPEKLASDLSPRLSAVHITPERQSKRRLFRDSGIEDEAENSIVQVQDDSSNVAGNK NGADAELYSLLHSNNRRAALLCKFKEKYGIPFNEITRTFKSNKSCTQNWIIVVFACAEDLIEASKTTMQN HVSYLQMITSDFSALYIICFKAAKSRETVVKLINSLLNTKEEQVLCDPPKIKSMAAALYCYKKVIADTCY KYGDFPDWIATHTVINHQLATADSFKFSDMVQWAYDNDMLDEAAIAYNYACYASENENAAAFLQTNSQLK YVKECCAMVRLYKKQEMRNMTMPEWIKSCFTNNYNSDDWKVIVRYLKYQNINFLEFLLALKLLLKGIPKK MCLVIYGPPDTGKSYFCYQFIQFMRGKVVSFMNKNSHFWLMPLLDSKIGFLDDATQCCWMYLDTHMRNAF DGNAVSVDVKHKNLQQIVLPPMLITTNCDVCRDPTFMYLRSRLTCFNFPNKLPLYENGEPKFKFTDNCWT SFFSKFWKHLDLPEDDPDGDTGNSERTFSCTTRHSIESD TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001691 |
| ORF Size | 1797 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001691.1, NP_043425 |
| RefSeq ORF | 1797 bp |
| Locus ID | 1403631 |
| MW | 68.9 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101964 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043423 |
CNY 3,800.00 |
|
| VC101965 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E7 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043424 |
CNY 3,800.00 |
|
| VC101967 | Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043426 |
CNY 4,750.00 |
|
| VC101968 | Myc-DDK-tagged ORF clone of viral ORF for putative, partial [Human papillomavirus type 50], codon optimized for human cell expression, NP_043427 |
CNY 3,800.00 |
|
| VC101969 | Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043428 |
CNY 6,180.00 |
|
| VC101970 | Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043429 |
CNY 6,270.00 |
