E1 (NC_001355) Virus Tagged ORF Clone

CAT＃: VC101994

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040298

Datasheet

SDS

View other clones from "Virus" (8)

Need custom modification / cloning service?
Get a free quote

CNY 7,790.00

货期*

5周

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101994 represents NCBI reference of NP_040298 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGATGACAGTGGCACGGAGAATGAAGGGAGTGGCTGCACGGGTTGGTTTATGGTGGAGGCGATTG TGCAGCATCCTACGGGGACACAGATTAGTGACGACGAAGACGAAGAAGTGGAAGACTCAGGATATGACAT GGTCGACTTTATCGATGATAGTAACATTACACATAATAGTCTGGAGGCTCAAGCTCTGTTCAACAGGCAG GAGGCCGACACTCATTACGCGACTGTTCAGGATCTGAAAAGGAAGTACCTCGGAAGCCCTTATGTGAGCC CCATCAACACCATTGCCGAAGCTGTCGAGTCCGAGATCAGCCCCCGGCTGGACGCCATTAAACTTACGAG ACAGCCTAAAAAAGTTAAAAGAAGGTTGTTTCAGACCCGGGAGCTGACTGACTCTGGCTACGGGTACTCA GAAGTCGAGGCTGGTACCGGAACACAGGTCGAAAAGCACGGAGTTCCGGAGAATGGCGGCGATGGACAGG AGAAGGACACAGGTAGGGATATCGAAGGAGAAGAGCATACTGAGGCTGAAGCGCCTACAAATAGCGTACG CGAGCACGCTGGCACTGCTGGAATCCTCGAACTTCTGAAATGCAAGGACCTTCGCGCAGCATTGCTCGGG AAATTTAAAGAGTGCTTTGGTCTGTCATTCATTGATCTTATAAGGCCCTTTAAGAGCGACAAAACCACGT GCTTGGATTGGGTGGTTGCTGGGTTTGGAATTCATCATTCTATATCCGAGGCCTTCCAGAAATTGATCGA ACCACTTAGCCTCTATGCTCACATTCAGTGGTTGACTAATGCTTGGGGCATGGTACTCCTTGTGCTGCTC AGATTCAAGGTGAATAAAAGCCGGTCCACTGTGGCTCGAACTCTGGCTACTTTGCTGAACATTCCCGAGA ATCAGATGCTGATTGAGCCCCCCAAGATCCAGAGCGGTGTGGCTGCTCTGTATTGGTTTAGAACGGGCAT CAGTAACGCAAGCACTGTTATTGGCGAGGCCCCCGAGTGGATCACCAGGCAAACCGTTATAGAACATGGG CTGGCCGATTCCCAGTTTAAGCTGACCGAGATGGTGCAGTGGGCATACGATAATGACATCTGTGAGGAGT CAGAGATTGCCTTTGAGTACGCTCAGAGAGGAGATTTCGATTCTAACGCCCGAGCATTCCTGAATAGCAA CATGCAGGCTAAATACGTCAAAGACTGCGCCACTATGTGCAGGCACTACAAACATGCTGAAATGAGAAAG ATGAGCATCAAGCAGTGGATCAAACATCGGGGAAGCAAAATTGAGGGCACTGGCAATTGGAAGCCCATTG TGCAGTTCCTCAGACACCAAAATATAGAGTTCATTCCCTTCCTTACAAAATTTAAGCTGTGGCTGCACGG CACCCCTAAGAAGAATTGCATAGCTATTGTCGGTCCCCCTGACACCGGTAAGTCCTATTTCTGTATGTCC CTGATTTCCTTCCTCGGGGGAACTGTCATTTCACACGTTAACTCTAGTAGCCATTTTTGGCTTCAGCCTC TGGTGGACGCAAAGGTGGCTCTGCTCGATGACGCTACTCAGCCGTGCTGGATATACATGGATACCTATAT GCGGAACCTCCTGGATGGTAACCCCATGAGTATCGATCGGAAACACAAAGCCCTGACGTTGATCAAATGC CCCCCATTGCTGGTGACTAGTAATATCGACATCACTAAGGAGGATAAATATAAGTACTTGCATACCCGCG TCACAACCTTCACATTTCCCAACCCTTTCCCCTTTGACCGCAACGGAAACGCCGTGTACGAGTTGAGTAA CACCAATTGGAAATGCTTCTTCGAGAGGCTCTCCTCCAGTCTGGACATTCAGGATAGCGAGGATGAAGAG GATGGCTCAAACAGCCAGGCCTTTAGATGCGTACCTGGTACTGTGGTCCGGACCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101994 representing NP_040298 Red=Cloning sites Green=Tags MADDSGTENEGSGCTGWFMVEAIVQHPTGTQISDDEDEEVEDSGYDMVDFIDDSNITHNSLEAQALFNRQ EADTHYATVQDLKRKYLGSPYVSPINTIAEAVESEISPRLDAIKLTRQPKKVKRRLFQTRELTDSGYGYS EVEAGTGTQVEKHGVPENGGDGQEKDTGRDIEGEEHTEAEAPTNSVREHAGTAGILELLKCKDLRAALLG KFKECFGLSFIDLIRPFKSDKTTCLDWVVAGFGIHHSISEAFQKLIEPLSLYAHIQWLTNAWGMVLLVLL RFKVNKSRSTVARTLATLLNIPENQMLIEPPKIQSGVAALYWFRTGISNASTVIGEAPEWITRQTVIEHG LADSQFKLTEMVQWAYDNDICEESEIAFEYAQRGDFDSNARAFLNSNMQAKYVKDCATMCRHYKHAEMRK MSIKQWIKHRGSKIEGTGNWKPIVQFLRHQNIEFIPFLTKFKLWLHGTPKKNCIAIVGPPDTGKSYFCMS LISFLGGTVISHVNSSSHFWLQPLVDAKVALLDDATQPCWIYMDTYMRNLLDGNPMSIDRKHKALTLIKC PPLLVTSNIDITKEDKYKYLHTRVTTFTFPNPFPFDRNGNAVYELSNTNWKCFFERLSSSLDIQDSEDEE DGSNSQAFRCVPGTVVRTL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_001355
ORF Size	1947 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001355.1, NP_040298
RefSeq ORF	1947 bp
Locus ID	1489363
MW	73.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC101992	Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040296	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,320.00
VC101993	Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040297	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,320.00
VC101995	Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040299	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00
VC101996	Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040300	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101997	Myc-DDK-tagged ORF clone of viral ORF for E5a protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040301	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101998	Myc-DDK-tagged ORF clone of viral ORF for E5b protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040302	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101999	Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040303	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,610.00
VC102000	Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040304	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,080.00

其它E1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies