United States
Germany
Japan
United Kingdom
China
E1 (NC_010329) Virus Tagged ORF Clone
CAT＃: VC102009
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672010
View other clones from "Virus" (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,320.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102009 represents NCBI reference of YP_001672010 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATCCAAATAAAGGAACAGACACCACCGATAACTTCGATTGCAACTCAGAGTGGCTCCTGGTCG ATGAGGCGGAATGCGTCGATGACCTGGATGTGTTGGATGAGCTGCTGGAAGAAAGTACTCAGTGTAGCAC TGTCAGCAATCTCATAGACGATGACCCCGTAGACATCGAAGACCAGGGTAACTCCCTCGCTCTTTTCAAT TCACAGGTGACCGAGGACTGTAATACTGCAATCTCCGCACTCAAGAGAAAGCTGATCAAGAGCCCTCAGC TCCAGACCGTGGCTGAGCTTAGTCCGAGATTGCAGGCTGTGAGCATTAGTCCCAAAAAACAGTCTAAACG CCGCCTGTTCGATGACTCCGGCATAGGGGAAGACGAAGCCCAGAACACATCACAGGTGGCCCTGGACAGC CTGGAATCCTCATTGGAAACAAGCGGGGCCGGCCTTACGATTAACCTGCTTCACTGTAGTAATAGACAGG CCACTGTGCATTTGAAATTCAAAGAGCTCTATGGAGTGTCATTCAAGGAACTGACGAGAAACTTCAAGTC CGATAAGTCCTGCAGTAACTCATGGGTAATCGCTGTGTACAATGCAGTTGAAGAAGTCCTGGAAGCATCT AAAGTTCAGCTGCAGAAGCATGTCGAGTTCTTGCAGCTTATCGTGTACGGATTCCATGGACTTTATCTGA TTGTGTTTAAGAGCACCAAGAACCGCGACACAGTGTCAAAACTTTTCACCGAAATGCTTAACGTCAATGC TCTTCAGCTGATGTGTGACCCACCCAGGACACGCTCAGTGCCTGTCGCATTGTTCTTTTACCGCAAGTCC CTGGGCAACGCGAGTTTTATGTACGGTGAGGTCCCAGATTGGATCAAGAAGCAGACCCTGGTCGAACACC AGTCAGCCTCCGCCGCCGAGACCTTCGATTTCTCTTCAATGGTGCAGTGGGCTTACGATAATGAATTCAC TGAAGAGGCCGAGATCGCTTATCAATATGCCCTCGCCGCAGACGAAGACCCTAACGCAGAAGCTTTCTTG AAGCATAATAACCAGTACAAATTCGTCAAGGATTGCGCTGCCATGGTCAAAATGTATAGACGGTACGAAC TCAGACAGATGTCTATGGCAGAGTGGATTGATAAATGCTGTTCAAAATGCACCGAAAATGGAAATTGGAA AATCATTGCGACATTCCTCAAACACCAGAACGTACAGTTTCTCTCCTTTCTTATTGCCCTGAAACCCTTC CTTCAGGGGGTGCCTAAGAAAAATTGCCTGGTGTTCGTCGGCCCACCTGACACTGGCAAGAGCTATTTCT GTTTTAGCCTTATCCGCTTTTTCGGGGGCAAAGTGGTGTCCTTCATGAACAGAAACAGTCATTTTTGGCT GCAGCCTCTGCAGGATACCAAATTGGGATTCCTGGACGACGCTACCCATCCCTGTTGGGTGTACATGGAC ATCAACCTGCGGAACGGCCTGGATGGCAACCTTGTCTCCCTTGATGCGAAACACCGCGCCCCGATGCAGA TGAAACTGCCACCATTGTTGGTGACAACTAATGTGGACGTCATGGAAGATCAATCACTGAGATATCTGCA TTCCCGCCTGGTATGCTTCAAATTTCCAAATAAACTCCCACTGAATGATGACGGCAGCCTGGTCTACGAG ATCACAAACGACACATGGGCTTGCTTCTTTCGCAAATTCGCTACCCAGCTGAACTTGGTGCTCGAGGATG GCGAGAATGGCAATACAGGTAACTCAGACCGCACATTTCGCTGCACTGCCAGATGCCATATCGAGTCAAA T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102009 representing YP_001672010
Red=Cloning sites Green=Tags MADPNKGTDTTDNFDCNSEWLLVDEAECVDDLDVLDELLEESTQCSTVSNLIDDDPVDIEDQGNSLALFN SQVTEDCNTAISALKRKLIKSPQLQTVAELSPRLQAVSISPKKQSKRRLFDDSGIGEDEAQNTSQVALDS LESSLETSGAGLTINLLHCSNRQATVHLKFKELYGVSFKELTRNFKSDKSCSNSWVIAVYNAVEEVLEAS KVQLQKHVEFLQLIVYGFHGLYLIVFKSTKNRDTVSKLFTEMLNVNALQLMCDPPRTRSVPVALFFYRKS LGNASFMYGEVPDWIKKQTLVEHQSASAAETFDFSSMVQWAYDNEFTEEAEIAYQYALAADEDPNAEAFL KHNNQYKFVKDCAAMVKMYRRYELRQMSMAEWIDKCCSKCTENGNWKIIATFLKHQNVQFLSFLIALKPF LQGVPKKNCLVFVGPPDTGKSYFCFSLIRFFGGKVVSFMNRNSHFWLQPLQDTKLGFLDDATHPCWVYMD INLRNGLDGNLVSLDAKHRAPMQMKLPPLLVTTNVDVMEDQSLRYLHSRLVCFKFPNKLPLNDDGSLVYE ITNDTWACFFRKFATQLNLVLEDGENGNTGNSDRTFRCTARCHIESN TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_010329 |
| ORF Size | 1821 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_010329.1, YP_001672010 |
| RefSeq ORF | 1821 bp |
| Locus ID | 5896661 |
| MW | 68.9 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102007 | Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672008 |
CNY 3,800.00 |
|
| VC102008 | Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672009 |
CNY 3,800.00 |
|
| VC102010 | Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672011 |
CNY 4,750.00 |
|
| VC102011 | Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672012 |
CNY 3,800.00 |
|
| VC102012 | Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672013 |
CNY 6,370.00 |
|
| VC102013 | Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672014 |
CNY 6,180.00 |
