L2 (NC_001596) Virus Tagged ORF Clone
CAT#: VC102019
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041865
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CNY 6,460.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC102019 represents NCBI reference of NP_041865 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGAGCGAAGCGGACCAAACGGGCCTCCGTCACAGACATCTACAGAGGATGTAAGGCTGCCGGCA CCTGCCCCCCCGACGTCATAAATAAGGTCGAGCATACAACCATAGCCGACAAAATTCTGCAGTATGGATC AGCCGGCGTGTTTTTCGGTGGGCTCGGCATCTCTACAGGCAGAGGAACGGGAGGTGCGACTGGTTACGTT CCCCTTGGCGAGGGACCAGGCGTCCGAGTGGGGGGCACTCCTACCATTGTGCGACCGGGCGTTATCCCCG AAATAATTGGCCCCACTGATTTGATCCCTCTGGATACCGTTCGGCCAATCGATCCTACTGCCCCATCAAT TGTGACCGGCACTGATTCTACCGTAGACCTGCTGCCAGGAGAGATTGAGTCAATAGCCGAAATACATCCT GTACCCGTGGACAACGCCGTGGTCGACACACCCGTGGTGACAGAAGGCCGAAGAGGCAGCAGTGCGATTC TGGAGGTAGCAGACCCTTCCCCACCCATGCGGACAAGAGTGGCTCGCACGCAATACCATAATCCTGCGTT CCAGATTATCTCTGAGTCCACACCTATGAGTGGTGAGTCAAGCCTCGCTGACCACATAATCGTGTTTGAA GGAAGCGGGGGACAGCTGGTTGGCGGACCAAGAGAGTCCTACACCGCAAGTTCCGAGAATATAGAACTGC AGGAGTTTCCCAGTCGGTACTCTTTTGAGATTGATGAGGGGACACCACCGCGAACATCCACCCCCGTCCA GCGGGCCGTTCAGTCTCTGAGCTCATTGAGAAGGGCTCTGTACAATAGACGCTTGACCGAACAGGTCGCT GTAACAGACCCCCTGTTTCTCTCTCGGCCATCAAGGTTGGTGCAGTTCCAATTTGACAACCCGGCTTTTG AGGACGAAGTGACACAGATCTTTGAAAGGGATCTCTCCACAGTGGAAGAGCCCCCTGACAGACAGTTCCT GGACGTGCAACGACTGAGCCGCCCGTTGTACACAGAAACACCCCAAGGTTATGTGCGCGTGAGCCGGCTG GGCAGGAGGGCAACAATACGCACCCGGTCCGGAGCTCAGGTGGGCGCCCAAGTGCACTTTTATAGAGATC TGTCCACAATTAATACAGAGGAACCCATCGAGATGCAGCTGCTGGGTGAACATTCCGGTGATAGCACTAT CGTGCAGGGTCCAGTGGAGAGTTCTATAGTCGATGTTAATATCGATGAACCCGATGGTTTGGAGGTGGGC AGGCAAGAGACTCCCTCCGTGGAAGACGTAGATTTTAATTCCGAGGACCTCCTGTTGGATGAGGGAGTAG AGGACTTCAGCGGGAGCCAGTTGGTAGTGGGGACCCGCAGGTCTACTAATACTCTCACAGTCCCTCGCTT CGAGACCCCACGGGACACCAGCTTTTATATTCAGGATATCCAAGGGTACACTGTCTCATACCCAGAATCC CGCCAGACCACAGATATCATCTTCCCTCATCCGGACACACCAACCGTCGTGATTCATATCAATGATACGA GCGGTGACTATTATCTGCATCCTTCCCTGCAGAGAAAGAAGAGAAAAAGAAAATACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102019 representing NP_041865
Red=Cloning sites Green=Tags MVRAKRTKRASVTDIYRGCKAAGTCPPDVINKVEHTTIADKILQYGSAGVFFGGLGISTGRGTGGATGYV PLGEGPGVRVGGTPTIVRPGVIPEIIGPTDLIPLDTVRPIDPTAPSIVTGTDSTVDLLPGEIESIAEIHP VPVDNAVVDTPVVTEGRRGSSAILEVADPSPPMRTRVARTQYHNPAFQIISESTPMSGESSLADHIIVFE GSGGQLVGGPRESYTASSENIELQEFPSRYSFEIDEGTPPRTSTPVQRAVQSLSSLRRALYNRRLTEQVA VTDPLFLSRPSRLVQFQFDNPAFEDEVTQIFERDLSTVEEPPDRQFLDVQRLSRPLYTETPQGYVRVSRL GRRATIRTRSGAQVGAQVHFYRDLSTINTEEPIEMQLLGEHSGDSTIVQGPVESSIVDVNIDEPDGLEVG RQETPSVEDVDFNSEDLLLDEGVEDFSGSQLVVGTRRSTNTLTVPRFETPRDTSFYIQDIQGYTVSYPES RQTTDIIFPHPDTPTVVIHINDTSGDYYLHPSLQRKKRKRKYL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001596 |
ORF Size | 1599 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001596.1, NP_041865 |
RefSeq ORF | 1599 bp |
Locus ID | 1489483 |
MW | 58.4 kDa |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
VC102014 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041860 |
CNY 3,800.00 |
|
VC102015 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041861 |
CNY 3,800.00 |
|
VC102016 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041862 |
CNY 3,800.00 |
|
VC102017 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041863 |
CNY 7,320.00 |
|
VC102018 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041864 |
CNY 5,610.00 |
|
VC102020 | Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041866 |
CNY 6,180.00 |