HN (NC_003461) Virus Tagged ORF Clone

CAT＃: VC102050

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for HN glycoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604441

Datasheet

SDS

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CNY 6,940.00

货期*

4周

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102050 represents NCBI reference of NP_604441 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAAAAGGGTAAGACAAATTCTTCATATTGGTCCACCACTAGAAACGATAATTCAACCGTGAATA CTCATATAAACACCCCAGCAGGTAGGACGCATATCTGGCTCTTGATCGCTACGACGATGCACACAGTGCT TTCATTTATTATCATGATCCTTTGTATCGATCTGATCATCAAGCAGGATACTTGTATGAAAACGAATATT ATGACCGTCTCCAGCATGAACGAATCCGCCAAGATTATAAAGGAGACCATCACAGAACTTATTCGCCAGG AAGTGATCTCACGAACTATCAACATCCAGTCCTCTGTCCAGTCAGGCATCCCGATCTTGCTGAATAAACA GTCCAGAGACCTGACCCAGCTGATTGAAAAAAGCTGCAACAGACAGGAGCTGGCTCAGATCTGCGAGAAT ACAATTGCAATTCACCATGCCGACGGAATTTCACCACTCGATCCCCACGACTTTTGGCGGTGTCCTGTCG GAGAACCACTGCTCTCTAATAATCCTAACATCTCACTGCTGCCTGGTCCCTCTCTTCTGTCAGGGTCAAC CACCATCTCTGGATGCGTCCGCTTGCCTTCACTTTCAATAGGAGATGCGATCTATGCTTATAGCTCAAAT CTGATTACTCAGGGTTGTGCAGATATTGGGAAGTCTTACCAAGTGCTGCAACTCGGATATATCTCACTTA ACTCTGATATGTATCCTGATCTTAATCCAGTTATTAGTCACACCTATGACATTAACGATAACAGAAAGAG CTGTTCTGTGATTGCAGCAGGGACTCGCGGGTACCAACTCTGTAGCCTGCCTACTGTGAACGAAACCACC GATTACTCCAGCGAGGGTATCGAAGACCTGGTGTTCGACATCCTGGATTTGAAAGGAAAAACCAAAAGTC ACAGGTATAAGAATGAAGATATCACCTTCGATCACCCTTTCTCAGCAATGTATCCTTCAGTGGGGTCTGG CATCAAAATCGAGAACACCCTCATATTCCTTGGGTATGGAGGGCTGACCACACCCCTGCAGGGGGATACC AAGTGTGTGATTAATAGGTGCACCAACGTTAACCAGTCCGTTTGCAATGACGCCCTGAAAATCACATGGT TGAAAAAAAGGCAGGTAGTGAACGTCCTCATCAGGATTAATAACTACCTGTCAGATAGGCCAAAAATTGT TGTGGAAACCATACCAATTACTCAGAACTACCTCGGCGCTGAGGGCCGGCTCCTCAAGCTGGGTAAAAAA ATATACATCTATACTCGGAGCTCCGGCTGGCACTCAAATCTCCAGATAGGCTCCTTGGACATCAACAATC CTATGACCATCAAGTGGGCACCGCACGAGGTCCTGTCACGCCCAGGAAATCAAGATTGTAATTGGTATAA TAGATGCCCACGCGAATGTATCAGCGGGGTCTATACTGATGCGTACCCCCTGAGCCCAGACGCTGTAAAT GTTGCCACGACCACCCTTTACGCAAATACAAGCCGCGTGAACCCAACTATTATGTACAGCAACACCAGCG AAATCATTAACATGCTGAGACTTAAAAACGTGCAGCTCGAGGCTGCATATACGACAACATCATGCATCAC ACACTTTGGAAAGGGCTACTGCTTTCACATCGTCGAGATTAATCAGGCAAGCCTCAACACACTGCAGCCC ATGCTCTTTAAGACCTCTATCCCAAAAATCTGTAAAATTACCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102050 representing NP_604441 Red=Cloning sites Green=Tags MAEKGKTNSSYWSTTRNDNSTVNTHINTPAGRTHIWLLIATTMHTVLSFIIMILCIDLIIKQDTCMKTNI MTVSSMNESAKIIKETITELIRQEVISRTINIQSSVQSGIPILLNKQSRDLTQLIEKSCNRQELAQICEN TIAIHHADGISPLDPHDFWRCPVGEPLLSNNPNISLLPGPSLLSGSTTISGCVRLPSLSIGDAIYAYSSN LITQGCADIGKSYQVLQLGYISLNSDMYPDLNPVISHTYDINDNRKSCSVIAAGTRGYQLCSLPTVNETT DYSSEGIEDLVFDILDLKGKTKSHRYKNEDITFDHPFSAMYPSVGSGIKIENTLIFLGYGGLTTPLQGDT KCVINRCTNVNQSVCNDALKITWLKKRQVVNVLIRINNYLSDRPKIVVETIPITQNYLGAEGRLLKLGKK IYIYTRSSGWHSNLQIGSLDINNPMTIKWAPHEVLSRPGNQDCNWYNRCPRECISGVYTDAYPLSPDAVN VATTTLYANTSRVNPTIMYSNTSEIINMLRLKNVQLEAAYTTTSCITHFGKGYCFHIVEINQASLNTLQP MLFKTSIPKICKITS TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_003461
ORF Size	1725 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_003461.1, NP_604441
RefSeq ORF	1725 bp
Locus ID	935266
MW	64.0 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC102042	Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604433	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,370.00
VC102043	Myc-DDK-tagged ORF clone of viral ORF for C' protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604434	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102044	Myc-DDK-tagged ORF clone of viral ORF for phosphoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604435	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,840.00
VC102045	Myc-DDK-tagged ORF clone of viral ORF for C protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604436	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00
VC102046	Myc-DDK-tagged ORF clone of viral ORF for Y1 protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604437	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102047	Myc-DDK-tagged ORF clone of viral ORF for putative Y2 protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604438	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102048	Myc-DDK-tagged ORF clone of viral ORF for matrix protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604439	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,180.00
VC102049	Myc-DDK-tagged ORF clone of viral ORF for F glycoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604440	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,136.00 CNY 6,750.00

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