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fusion protein (NC_001796) Virus Tagged ORF Clone
CAT＃: VC102062
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for fusion protein [Human parainfluenza virus 3], codon optimized for human cell expression, NP_067151
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CNY 6,560.00
| Cited in 8 publications. |
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102062 represents NCBI reference of NP_067151 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACAAGCACACTGCTGATCATCACGACTATCATCATGGCGTCATTTTGCCAGATCGACATCACCA AACTGCAACACGTAGGAGTTCTGGTCAACTCCCCAAAAGGAATGAAAATCAGCCAGAATTTTGAAACCAG ATATTTGATCCTGTCCCTGATCCCTAAGATTGAGGATTCCAACTCCTGTGGAGATCAGCAAATCAAGCAG TACAAGAGACTTCTGGACCGGCTGATCATTCCTTTGTACGACGGACTCCGGCTCCAGAAGGACGTGATAG TGACCAACCAAGAATCTAATGAGAACACCAATCCAAGGACAAAACGATTCTTTGGCGGCGTTATTGGGAC CATTGCCTTGGGTGTGGCTACCAGTGCGCAGATAACTGCAGCTGTTGCCCTGGTCGAGGCCAAACAGGCC CGGTCTGACATCGAAAAGCTTAAGGAGGCTATTCGCGACACAAACAAGGCCGTCCAGAGCGTTCAGTCTA GCATCGGGAACCTGATCGTCGCGATAAAGTCCGTTCAAGATTACGTGAACAAGGAGATTGTACCATCCAT CGCTCGATTGGGATGCGAGGCCGCGGGACTCCAACTTGGGATTGCTTTGACCCAGCATTACTCCGAGCTC ACTAACATCTTCGGCGACAACATCGGTTCTCTTCAGGAGAAGGGTATTAAACTGCAGGGGATAGCCAGTT TGTACCGAACCAATATAACTGAGATCTTTACGACCTCAACAGTGGACAAGTACGATATCTATGATCTGCT TTTCACTGAGAGCATCAAAGTGCGAGTTATCGACGTGGACCTCAACGATTACAGTATTACATTGCAGGTC AGACTGCCTCTTCTTACCAGGCTGCTGAATACCCAGATTTACAAAGTGGACAGTATTTCATATAACATAC ATAATCGCGAGTGGTATATCCCCCTTCCCAGTCATATAATGACAAAGGGAGCCTTCCTCGGCGGCGCCGA TGTCAAAGAATGCATCGAGGCCTTCAGCAGCTACATCTGCCCAAGTGACCCTGGCTTTGTATTGAACCAC GAGATGGAGTCTTGCCTGAGCGGGAATATCTCACAGTGCCCACGCACAACGATCACTAGCGACATCGTGC CCAGATACGCCTTTGTGAACGGAGGAGTGGTTGCCAATTGTATCACCACGACTTGTACCTGTAATGGCAT CGGCAACAGAATCAACCAACCACCAAATCAGGGGGTGAAAATCATTACCCATAAGGAGTGTTCCACCATT GGGATCAACGGAATGCTTTTTAACACCAATAAAGAGGGGACGCTCGCCTTCTATACACCCGATGATATCA CTTTGAACAATTCAGTGGCGCTGGATCCTATCGACATTAGCATCGAGCTGAACAAAGCCAAGTCTGACCT GGAAGAGAGCAAAGAATGGATTCGCAAAAGCAATCAGAAATTGGATTCTATAGGGAACTGGCATCAGTCT AGTACAACCATTATCATAATACTCATGATGATAATTATCCTGTTTATTATCAATATCACTATTATTACGA TTGCTATTAAATACTACAGAATCCAGAAACGGAATCAGATGGACCAGAACGATAAGCCATACGTCCTGAC AAATAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102062 representing NP_067151
Red=Cloning sites Green=Tags MPTSTLLIITTIIMASFCQIDITKLQHVGVLVNSPKGMKISQNFETRYLILSLIPKIEDSNSCGDQQIKQ YKRLLDRLIIPLYDGLRLQKDVIVTNQESNENTNPRTKRFFGGVIGTIALGVATSAQITAAVALVEAKQA RSDIEKLKEAIRDTNKAVQSVQSSIGNLIVAIKSVQDYVNKEIVPSIARLGCEAAGLQLGIALTQHYSEL TNIFGDNIGSLQEKGIKLQGIASLYRTNITEIFTTSTVDKYDIYDLLFTESIKVRVIDVDLNDYSITLQV RLPLLTRLLNTQIYKVDSISYNIHNREWYIPLPSHIMTKGAFLGGADVKECIEAFSSYICPSDPGFVLNH EMESCLSGNISQCPRTTITSDIVPRYAFVNGGVVANCITTTCTCNGIGNRINQPPNQGVKIITHKECSTI GINGMLFNTNKEGTLAFYTPDDITLNNSVALDPIDISIELNKAKSDLEESKEWIRKSNQKLDSIGNWHQS STTIIIILMMIIILFIINITIITIAIKYYRIQKRNQMDQNDKPYVLTNK TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001796 |
| ORF Size | 1617 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001796.2, NP_067151 |
| RefSeq ORF | 1617 bp |
| Locus ID | 911957 |
| MW | 60.0 kDa |
Citations (8)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Tumor cell IDO enhances immune suppression and decreases survival independent of tryptophan metabolism in glioblastoma
,null,
Clinical cancer research : an official journal of the American Association for Cancer Research
,PubMed ID 34479957
[fusion protein]
|
|
An ACE2/Mas-related receptor MrgE axis in dopaminergic neuron mitochondria
,null,
Redox Biology
,PubMed ID 34333284
[fusion protein]
|
|
Angiotensin type 2 receptors: Role in aging and neuroinflammation in the substantia nigra.
,null,
Brain, behavior, and immunity
,PubMed ID 31863823
[fusion protein]
|
|
The intracellular angiotensin system buffers deleterious effects of the extracellular paracrine system
,null,
Cell Death & Disease
,PubMed ID 28880266
[fusion protein]
|
|
Mitochondrial angiotensin receptors in dopaminergic neurons. Role in cell protection and aging-related vulnerability to neurodegeneration
,null,
Cell Death & Disease
,PubMed ID 27763643
[fusion protein]
|
|
Crosstalk between insulin-like growth factor-1 and angiotensin-II in dopaminergic neurons and glial cells: role in neuroinflammation and aging
,null,
Oncotarget
,PubMed ID 27167199
[fusion protein]
|
|
Reciprocal regulation between sirtuin-1 and angiotensin-II in the substantia nigra: implications for aging and neurodegeneration
,null,
Oncotarget
,PubMed ID 26384348
[fusion protein]
|
|
Effects of alternative splicing on function of Bestrophin-1 calcium-activated chloride channels
,null,
The Biochemical journal
,PubMed ID 24341532
[fusion protein]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102059 | Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Human parainfluenza virus 3], codon optimized for human cell expression, NP_067148 |
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|
| VC102060 | Myc-DDK-tagged ORF clone of viral ORF for phosphoprotein [Human parainfluenza virus 3], codon optimized for human cell expression, NP_067149 |
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|
| VC102061 | Myc-DDK-tagged ORF clone of viral ORF for matrix potein [Human parainfluenza virus 3], codon optimized for human cell expression, NP_067150 |
CNY 4,180.00 |
|
| VC102063 | Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin-neuraminidase [Human parainfluenza virus 3], codon optimized for human cell expression, NP_067152 |
CNY 6,940.00 |
|
| VC102065 | Myc-DDK-tagged ORF clone of viral ORF for C protein [Human parainfluenza virus 3], codon optimized for human cell expression, NP_599251 |
CNY 3,800.00 |
|
| VC102066 | Myc-DDK-tagged ORF clone of viral ORF for D protein [Human parainfluenza virus 3], codon optimized for human cell expression, NP_599250 |
CNY 4,470.00 |
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