VP1 (NC_000883) Virus Tagged ORF Clone

CAT#: VC102081

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1 [Human parvovirus B19], codon optimized for human cell expression, YP_004928146



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CNY 11,880.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102081 represents NCBI reference of YP_004928146 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAAAAGAAAGCGGCAAATGGTGGGAGTCTGATGATGAGTTTGCTAAGGCTGTGTATCAGCAGTTTG
TGGAGTTCTATGAGAAAGTTACTGGGACTGATCTCGAGTTGATTCAGATCCTGAAGGACCACTACAACAT
ATCCCTGGATAATCCACTGGAAAATCCCTCCTCACTGTTTGACTTGGTCGCTCGCATTAAGAATAACTTG
AAGAATAGTCCAGATCTGTACTCCCATCATTTCCAGAGCCACGGACAGCTGTCCGATCACCCTCACGCGC
TGTCAAGTTCCTCAAGTCACGCAGAACCTCGCGGAGAGGACGCAGTGCTCTCTTCTGAGGACCTCCATAA
ACCCGGGCAAGTCTCAGTTCAACTCCCCGGGACCAACTACGTGGGACCTGGCAACGAACTCCAAGCGGGA
CCTCCCCAGAGCGCCGTCGACAGCGCCGCAAGAATTCATGACTTCCGCTACAGTCAGCTTGCTAAACTGG
GGATCAACCCCTACACCCACTGGACTGTGGCCGACGAGGAACTCCTGAAAAACATTAAAAATGAGACCGG
ATTTCAAGCCCAAGTCGTGAAGGACTACTTCACACTGAAAGGGGCTGCTGCTCCAGTGGCCCATTTCCAA
GGAAGCCTGCCAGAGGTTCCAGCATATAATGCTTCTGAGAAATACCCCTCTATGACATCAGTGAATTCTG
CTGAAGCGAGTACAGGCGCAGGAGGAGGTGGTAGTAACCCTGTCAAAAGTATGTGGTCTGAGGGAGCCAC
TTTCAGTGCAAACTCAGTTACCTGTACGTTTTCAAGGCAGTTTCTGATCCCATATGATCCTGAACACCAT
TACAAGGTATTTTCACCAGCCGCATCTTCATGTCACAATGCATCAGGCAAGGAAGCTAAGGTGTGCACCA
TCTCACCCATCATGGGATACTCTACACCCTGGCGGTACCTGGATTTTAATGCACTGAACCTGTTCTTCTC
CCCCCTGGAATTTCAGCATTTGATCGAGAACTACGGTTCAATAGCCCCCGATGCCCTGACCGTGACTATT
AGTGAGATCGCCGTCAAGGATGTGACCGACAAGACTGGTGGTGGAGTGCAAGTGACCGACTCTACCACAG
GCCGGCTGTGTATGCTGGTGGACCACGAATACAAATATCCCTATGTCCTGGGCCAAGGCCAGGATACGCT
TGCTCCCGAATTGCCGATTTGGGTTTACTTCCCGCCTCAGTACGCTTACTTGACCGTCGGTGACGTGAAT
ACCCAGGGCATTTCCGGAGACTCCAAGAAGCTGGCTTCTGAGGAGTCAGCTTTTTATGTGCTGGAGCACT
CCTCTTTCCAGCTTTTGGGAACCGGTGGGACAGCTACCATGTCCTACAAATTTCCGCCCGTACCACCAGA
AAATCTGGAAGGATGTAGTCAGCATTTCTATGAGATGTATAACCCCCTTTACGGCTCTCGCTTGGGGGTG
CCGGACACACTGGGCGGCGACCCTAAATTCAGATCACTGACTCACGAGGATCATGCGATCCAACCTCAGA
ACTTCATGCCCGGCCCCCTCGTGAACAGCGTGTCTACCAAGGAAGGCGACTCCTCTAACACCGGAGCAGG
CAAGGCACTGACCGGGCTCAGCACAGGAACAAGCCAGAACACCAGGATCAGCCTCCGGCCCGGCCCAGTG
AGCCAGCCATATCACCACTGGGATACCGATAAGTACGTCACCGGTATCAACGCTATCTCTCACGGCCAGA
CTACATACGGAAATGCCGAGGACAAGGAATATCAGCAGGGCGTCGGACGCTTTCCTAACGAGAAGGAGCA
GCTGAAGCAGCTTCAAGGCCTGAATATGCACACATACTTTCCCAATAAAGGCACCCAACAGTACACGGAC
CAGATCGAGCGCCCACTGATGGTGGGTTCTGTATGGAACAGGAGGGCGCTGCACTACGAAAGCCAGCTGT
GGAGCAAAATACCTAACCTCGACGACAGCTTCAAAACTCAGTTCGCTGCCTTGGGTGGCTGGGGCCTGCA
TCAGCCACCACCCCAGATTTTCTTGAAGATCCTCCCACAATCAGGACCTATTGGCGGAATCAAAAGTATG
GGCATCACCACTCTGGTACAGTATGCCGTGGGGATTATGACCGTGACTATGACTTTCAAGCTCGGCCCAC
GAAAAGCCACCGGGCGATGGAACCCCCAACCTGGCGTGTACCCACCACACGCAGCCGGGCACCTTCCATA
CGTGCTGTATGATCCCACCGCAACGGACGCTAAACAACACCACCGCCATGGGTACGAGAAACCTGAGGAG
CTCTGGACGGCCAAATCAAGGGTGCACCCACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102081 representing YP_004928146
Red=Cloning sites Green=Tags

MSKESGKWWESDDEFAKAVYQQFVEFYEKVTGTDLELIQILKDHYNISLDNPLENPSSLFDLVARIKNNL
KNSPDLYSHHFQSHGQLSDHPHALSSSSSHAEPRGEDAVLSSEDLHKPGQVSVQLPGTNYVGPGNELQAG
PPQSAVDSAARIHDFRYSQLAKLGINPYTHWTVADEELLKNIKNETGFQAQVVKDYFTLKGAAAPVAHFQ
GSLPEVPAYNASEKYPSMTSVNSAEASTGAGGGGSNPVKSMWSEGATFSANSVTCTFSRQFLIPYDPEHH
YKVFSPAASSCHNASGKEAKVCTISPIMGYSTPWRYLDFNALNLFFSPLEFQHLIENYGSIAPDALTVTI
SEIAVKDVTDKTGGGVQVTDSTTGRLCMLVDHEYKYPYVLGQGQDTLAPELPIWVYFPPQYAYLTVGDVN
TQGISGDSKKLASEESAFYVLEHSSFQLLGTGGTATMSYKFPPVPPENLEGCSQHFYEMYNPLYGSRLGV
PDTLGGDPKFRSLTHEDHAIQPQNFMPGPLVNSVSTKEGDSSNTGAGKALTGLSTGTSQNTRISLRPGPV
SQPYHHWDTDKYVTGINAISHGQTTYGNAEDKEYQQGVGRFPNEKEQLKQLQGLNMHTYFPNKGTQQYTD
QIERPLMVGSVWNRRALHYESQLWSKIPNLDDSFKTQFAALGGWGLHQPPPQIFLKILPQSGPIGGIKSM
GITTLVQYAVGIMTVTMTFKLGPRKATGRWNPQPGVYPPHAAGHLPYVLYDPTATDAKQHHRHGYEKPEE
LWTAKSRVHPL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000883
ORF Size 2343 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000883.2, YP_004928146
RefSeq ORF 2343 bp
Locus ID 1489598
MW 86.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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