gp46 SU (NC_001436) Virus Tagged ORF Clone

CAT#: VC102150

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for gp46 SU [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_955620



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CNY 5,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102150 represents NCBI reference of NP_955620 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGGCAAATTTCTTGCAACCTTGATCCTCTTCTTCCAATTCTGCCCACTGATCTTGGGAGATTATA
GCCCCTCATGCTGCACTCTTACCGTAGGCGTATCTTCATATCATTCCAAGCCTTGCAATCCTGCCCAGCC
CGTCTGCTCCTGGACGCTGGACCTCCTGGCACTGTCTGCAGACCAGGCACTGCAGCCCCCGTGTCCAAAT
CTTGTCAGCTACAGTTCATACCACGCTACATACAGTCTGTACCTCTTTCCCCACTGGATCAAGAAGCCTA
ACCGGAACGGAGGAGGCTACTATTCAGCTAGTTACTCCGACCCTTGTTCACTGAAATGCCCATATCTGGG
CTGTCAAAGTTGGACATGCCCCTATACTGGTGCAGTTTCCTCCCCTTACTGGAAGTTCCAGCAAGATGTG
AACTTCACTCAGGAGGTGAGCCACTTGAACATCAACCTGCATTTCAGCAAGTGCGGATTCAGCTTTAGCC
TTCTCGTTGATGCTCCCGGCTACGATCCCATCTGGTTTCTGAATACTGAACCATCCCAGCTCCCGCCCAC
AGCCCCTCCACTGCTGAGCCACTCAAACCTGGATCACATCCTCGAGCCTTCAATTCCTTGGAAGTCCAAA
CTTCTGACCTTGGTACAACTCACGCTTCAGTCCACAAATTACACCTGCATCGTCTGCATTGACCGAGCCA
GCTTGTCCACTTGGCACGTTCTTTACTCCCCAAACGTCTCTGTCCCTAGCCCCAGTAGTACTCCCCTTTT
GTATCCTTCTTTGGCCCTGCCTGCCCCACACCTTACCCTCCCCTTCAATTGGACACATTGTTTCGACCCC
CAAATCCAGGCCATAGTTTCCTCCCCATGCCATAACTCTTTGATCCTCCCCCCGTTTTCTCTGTCTCCTG
TGCCCACCCTGGGATCCCGCTCCCGGAGAGCTGTTCCAGTCGCCGTCTGGCTGGTTAGCGCCTTGGCCAT
GGGAGCAGGAGTCGCTGGCCGGATTACTGGCTCCATGTCACTGGCCAGTGGTAAGTCTCTCCTGCACGAA
GTTGACAAAGATATCTCTCAGCTGACCCAAGCTATTGTCAAAAACCACAAGAACCTGCTGAAGATTGCAC
AATACGCTGCTCAGAACAGAAGAGGGTTGGATCTCCTCTTTTGGGAGCAGGGGGGCCTCTGTAAAGCCCT
CCAGGAACAATGTTGTTTCCTGAATATCACCAACAGCCACGTATCTATTCTGCAGGAACGACCACCACTG
GAGAATCGCGTGTTGACAGGATGGGGACTGAATTGGGACTTGGGCCTGAGTCAGTGGGCACGAGAAGCCC
TGCAGACCGGAATAACACTTGTGGCGCTCTTGCTGCTGGTCATCCTGGCCGGGCCTTGCATCCTTAGGCA
GCTCAGACATCTGCCATCAAGAGTCCGGTACCCTCATTATTCACTGATCAATCCCGAGTCAAGCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102150 representing NP_955620
Red=Cloning sites Green=Tags

MMGKFLATLILFFQFCPLILGDYSPSCCTLTVGVSSYHSKPCNPAQPVCSWTLDLLALSADQALQPPCPN
LVSYSSYHATYSLYLFPHWIKKPNRNGGGYYSASYSDPCSLKCPYLGCQSWTCPYTGAVSSPYWKFQQDV
NFTQEVSHLNINLHFSKCGFSFSLLVDAPGYDPIWFLNTEPSQLPPTAPPLLSHSNLDHILEPSIPWKSK
LLTLVQLTLQSTNYTCIVCIDRASLSTWHVLYSPNVSVPSPSSTPLLYPSLALPAPHLTLPFNWTHCFDP
QIQAIVSSPCHNSLILPPFSLSPVPTLGSRSRRAVPVAVWLVSALAMGAGVAGRITGSMSLASGKSLLHE
VDKDISQLTQAIVKNHKNLLKIAQYAAQNRRGLDLLFWEQGGLCKALQEQCCFLNITNSHVSILQERPPL
ENRVLTGWGLNWDLGLSQWAREALQTGITLVALLLLVILAGPCILRQLRHLPSRVRYPHYSLINPESSL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001436
ORF Size 1467 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001436.1, NP_955620
RefSeq ORF 1467 bp
MW 54.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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