37 kDa coat protein (NC_002190) Virus Tagged ORF Clone

CAT#: VC102277

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for 37 kDa coat protein [Infectious hypodermal and hematopoietic necrosis virus], codon optimized for human cell expression, YP_003572962



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

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经常一起买 (4)
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102277 represents NCBI reference of YP_003572962 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGCAGATTCCACACGGGCTAGTCCCCGAAAAAGAAGCAGGCGCGATGCACACAACGAGGATGAAG
AACACGCGGAGGGCTCCAGCGGACCAGATCCCCATCGATGTCTCCAGTTCAATACAGGGGACAGTATCCA
TATTACCTTCCAGACTAGGCGCTACTTTGAATTCGACGCTGCTAACGACGGCAACTTTGATGGCAAAAAC
CTGTATTGTCTGCCCCTCCACTGGATGAACCTCTACCTCTATGGACTGAAATCATCCGATAGCTCTGCTA
CAGAGACACAGAGGTACAAGATGGTCAAGTCAATGATGAAAACATACGGGTGGAAAGTGCACAAAGCCGG
CGTAGTGATGCACTCAATGGTCCCACTCATGAAAGACCTTAAGGTGTCTGGTGGAACCTCCTTCGAGACC
CTGACATTTACAGATACGCCCTACCTCGAGATCTTTAAAGACACTACCGGTCTCCACAACCAACTCAGTA
CTAAAGAAGCTGACGTGACCCTGGCTAAATGGATTCAAAATCCCCAACTGGTGACTGTGCAGAGTACTGC
TGCAAACTATGAGGATCCCATCCAGCAGTTCGGCTTCATGGAACAGATGAGGACCGGCGACCGCAAAGCA
TACACTATCCACGGTGACACCCGAAATTGGTACGGCGGAGAGATTCCTACAACCGGCCCAACTTTTATTC
CCAAGTGGGGTGGCCAACTGAAATGGGATAAGCCCAGCCTCGGGAACCTGGTTTATCCCGCTGACCACCA
CACTAATGATTGGCAGCAGATCTTCATGCGGATGTCCCCTATCAAAGGCCCCAATGGTGATGAGCTGAAG
CTGGGATGCCGAGTCCAGGCAGACTTCTTCCTGCACCTGGAGGTTCGCTTGCCTCCCCAGGGTTGCGTGG
CAAGTCTCGGAATGCTCCAGTATCTGCACGCTCCATGCACAGGTCAGCTTAATAAGTGTTACATCATGCA
TACAAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102277 representing YP_003572962
Red=Cloning sites Green=Tags

MCADSTRASPRKRSRRDAHNEDEEHAEGSSGPDPHRCLQFNTGDSIHITFQTRRYFEFDAANDGNFDGKN
LYCLPLHWMNLYLYGLKSSDSSATETQRYKMVKSMMKTYGWKVHKAGVVMHSMVPLMKDLKVSGGTSFET
LTFTDTPYLEIFKDTTGLHNQLSTKEADVTLAKWIQNPQLVTVQSTAANYEDPIQQFGFMEQMRTGDRKA
YTIHGDTRNWYGGEIPTTGPTFIPKWGGQLKWDKPSLGNLVYPADHHTNDWQQIFMRMSPIKGPNGDELK
LGCRVQADFFLHLEVRLPPQGCVASLGMLQYLHAPCTGQLNKCYIMHTN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_002190
ORF Size 987 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_002190.2, YP_003572962
RefSeq ORF 987 bp
Locus ID 1457864
MW 37.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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