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RNA-dependent RNA polymerase NS5 (NC_012532) Virus Tagged ORF Clone
CAT＃: VC102538
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS5 [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227205.1
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CNY 7,280.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZIKV_gp1, ZIKV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102538 represents NCBI reference of YP_009227205 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGGGGGACCGGGGAAACTCTGGGTGAAAAATGGAAGGCCCGGCTCAATCAAATGAGTGCACTGG AATTTTACAGCTATAAAAAATCCGGGATAACAGAAGTGTGTAGGGAGGAGGCCAGACGCGCACTCAAGGA CGGAGTTGCAACAGGAGGACACGCCGTTAGTAGGGGAAGTGCAAAAATACGGTGGCTCGAGGAACGCGGC TATTTGCAACCCTACGGAAAAGTGGTGGACTTGGGCTGTGGGCGAGGTGGCTGGTCTTATTACGCCGCAA CAATTCGAAAAGTCCAGGAAGTTCGAGGTTACACCAAAGGAGGACCTGGCCATGAGGAACCTATGCTTGT CCAAAGTTACGGGTGGAATATTGTGCGCCTGAAAAGCGGGGTCGACGTCTTTCACATGGCTGCGGAACCA TGTGATACCCTTCTTTGTGATATCGGTGAATCATCCTCTTCCCCTGAGGTCGAAGAAACACGGACACTCA GAGTACTTAGCATGGTTGGAGACTGGCTGGAAAAGAGACCAGGAGCATTCTGCATTAAGGTCTTGTGCCC CTATACATCCACCATGATGGAAACCATGGAACGGCTTCAGCGCAGGCATGGAGGCGGACTTGTGAGGGTG CCCTTGTGTCGCAACTCAACTCACGAAATGTACTGGGTGTCTGGTGCAAAGTCTAACATCATCAAATCCG TAAGCACAACATCACAGCTGCTTCTGGGGAGAATGGATGGACCTAGGAGACCAGTGAAGTATGAAGAGGA CGTCAACCTCGGCAGCGGCACCCGCGCGGTGGCGTCATGTGCGGAGGCCCCCAACATGAAAATCATAGGA AGAAGAATTGAAAGGATACGGAACGAGCATGCCGAAACATGGTTCCTTGACGAGAATCACCCTTACCGGA CATGGGCCTACCACGGATCCTACGAGGCGCCCACTCAGGGTAGTGCTTCATCCCTTGTCAACGGCGTGGT AAGGCTGCTTTCCAAACCTTGGGACGTCGTTACAGGAGTTACAGGAATAGCCATGACTGATACAACCCCT TACGGACAGCAGAGAGTGTTCAAAGAGAAGGTGGACACTCGAGTCCCCGATCCTCAGGAAGGAACACGGC AGGTAATGAACATTGTGTCTAGTTGGCTGTGGAAGGAGCTGGGGAAGCGAAAGAGACCGCGCGTATGTAC AAAAGAGGAGTTTATTAACAAAGTCCGCAGTAATGCTGCGTTGGGCGCAATCTTTGAAGAGGAAAAGGAA TGGAAAACCGCCGTTGAAGCTGTGAACGACCCAAGATTCTGGGCCCTTGTAGATCGCGAGCGGGAACATC ATCTGAGAGGTGAATGCCATTCCTGTGTGTATAACATGATGGGCAAACGAGAAAAGAAACAAGGGGAGTT CGGGAAGGCTAAAGGTTCACGAGCCATTTGGTATATGTGGTTGGGAGCTAGGTTCTTGGAGTTCGAAGCC CTGGGATTCCTGAACGAAGATCATTGGATGGGTCGCGAGAACAGTGGAGGAGGGGTCGAAGGTCTGGGAT TGCAGCGGTTGGGTTACATCTTGGAGGAAATGAACAGAGCTCCAGGGGGCAAAATGTATGCTGATGATAC TGCTGGATGGGATACTCGAATAAGCAAGTTCGATCTGGAGAACGAGGCTCTGATTACAAATCAGATGGAG GAAGGGCATCGCACACTGGCGTTGGCCGTCATCAAATATACGTATCAGAACAAGGTCGTGAAAGTCCTGC GCCCAGCGGAGGGAGGCAAAACTGTTATGGATATCATCTCTAGACAGGATCAGAGAGGTAGCGGGCAAGT CGTGACATATGCCCTCAACACCTTTACAAATCTGGTTGTACAGCTCATTAGGAACATGGAAGCCGAAGAA GTACTGGAAATGCAAGATCTTTGGCTCCTGCGCAAGCCAGAAAAGGTGACAAGGTGGCTCCAGAGTAACG GGTGGGATAGATTGAAGAGAATGGCCGTATCAGGAGACGACTGTGTCGTCAAACCTATCGATGACAGGTT CGCGCACGCACTCAGGTTCCTGAATGATATGGGAAAGGTAAGGAAGGACACTCAGGAATGGAAACCATCC ACCGGCTGGTCTAATTGGGAGGAAGTACCATTTTGCTCTCATCATTTTAACAAACTCTATCTGAAGGATG GAAGAAGTATCGTGGTCCCATGTCGCCACCAGGACGAATTGATTGGGAGGGCTCGGGTCAGTCCCGGAGC TGGCTGGTCAATAAGAGAAACTGCATGCTTGGCCAAATCATACGCTCAGATGTGGCAGCTGCTCTATTTC CACAGGCGAGACCTCAGACTGATGGCCAACGCGATCTGTAGTGCAGTTCCAGTAGACTGGGTCCCAACCG GGCGCACGACCTGGTCTATCCATGGCAAGGGGGAATGGATGACAACGGAGGATATGCTGATGGTCTGGAA TCGCGTGTGGATCGAGGAAAATGACCATATGGAGGACAAAACCCCTGTGACCAAGTGGACTGATATTCCG TACCTCGGCAAGCGCGAAGATCTGTGGTGTGGGAGCCTTATCGGACATCGGCCTCGCACAACCTGGGCAG AGAATATTAAAGACACCGTTAATATGGTTAGAAGGATCATAGGTGACGAAGAAAAGTACATGGATTACCT GTCAACCCAGGTGAGGTATCTCGGGGAGGAAGGGAGCACTCCCGGCGTGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102538 representing YP_009227205
Red=Cloning sites Green=Tags MGGGTGETLGEKWKARLNQMSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKIRWLEERG YLQPYGKVVDLGCGRGGWSYYAATIRKVQEVRGYTKGGPGHEEPMLVQSYGWNIVRLKSGVDVFHMAAEP CDTLLCDIGESSSSPEVEETRTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMMETMERLQRRHGGGLVRV PLCRNSTHEMYWVSGAKSNIIKSVSTTSQLLLGRMDGPRRPVKYEEDVNLGSGTRAVASCAEAPNMKIIG RRIERIRNEHAETWFLDENHPYRTWAYHGSYEAPTQGSASSLVNGVVRLLSKPWDVVTGVTGIAMTDTTP YGQQRVFKEKVDTRVPDPQEGTRQVMNIVSSWLWKELGKRKRPRVCTKEEFINKVRSNAALGAIFEEEKE WKTAVEAVNDPRFWALVDREREHHLRGECHSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGARFLEFEA LGFLNEDHWMGRENSGGGVEGLGLQRLGYILEEMNRAPGGKMYADDTAGWDTRISKFDLENEALITNQME EGHRTLALAVIKYTYQNKVVKVLRPAEGGKTVMDIISRQDQRGSGQVVTYALNTFTNLVVQLIRNMEAEE VLEMQDLWLLRKPEKVTRWLQSNGWDRLKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPS TGWSNWEEVPFCSHHFNKLYLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYF HRRDLRLMANAICSAVPVDWVPTGRTTWSIHGKGEWMTTEDMLMVWNRVWIEENDHMEDKTPVTKWTDIP YLGKREDLWCGSLIGHRPRTTWAENIKDTVNMVRRIIGDEEKYMDYLSTQVRYLGEEGSTPGVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_012532 |
| ORF Size | 2712 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_012532.1, YP_009227205 |
| RefSeq ORF | 2712 bp |
| MW | 103.1 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102527 | Myc-DDK-tagged ORF clone of viral ORF for anchored capsid protein C [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227206.1 |
CNY 3,800.00 |
|
| VC102528 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein C [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227196.1 |
CNY 1,320.00 |
|
| VC102529 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein precursor M [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227197.1 |
CNY 2,640.00 |
|
| VC102530 | Myc-DDK-tagged ORF clone of viral ORF for envelope protein E [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227198.1 |
CNY 5,968.00 |
|
| VC102531 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS1 [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227199.1 |
CNY 5,488.00 |
|
| VC102532 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2A [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227200.1 |
CNY 2,640.00 |
|
| VC102533 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2B [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227201.1 |
CNY 1,320.00 |
|
| VC102534 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS3 [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227202.1 |
CNY 5,064.00 |
|
| VC102535 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4A [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227203.1 |
CNY 1,920.00 |
|
| VC102536 | Myc-DDK-tagged ORF clone of viral ORF for Protein 2K [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227209.1 |
CNY 3,800.00 |
|
| VC102537 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4B [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227204.1 |
CNY 3,600.00 |
|
| VC102539 | Myc-DDK-tagged ORF clone of viral ORF for Membrane glycoprotein M [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227208.1 |
CNY 3,800.00 |
|
| VC102540 | Myc-DDK-tagged ORF clone of viral ORF for Protein pr [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227207.1 |
CNY 3,800.00 |
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