nonstructural protein NS3 (NC_001474) Virus Tagged ORF Clone

CAT#: VC102549

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS3 [Dengue virus 2], codon optimized for human cell expression, NP_739587



  View other clones from "Virus" (13)

Need custom modification / cloning service?
Get a free quote

CNY 7,320.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Synonyms DENV_gp1, Dengue, DENV
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102549 represents NCBI reference of NP_739587 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGTGTTCTCTGGGACGTGCCTTCCCCCCCACCAATGGGAAAAGCAGAACTTGAAGATGGCGCAT
ATAGAATTAAGCAGAAAGGAATCCTTGGGTACTCACAAATCGGGGCTGGCGTTTATAAGGAAGGGACCTT
TCATACTATGTGGCACGTGACCCGGGGCGCTGTCCTTATGCACAAGGGCAAGAGAATCGAACCCTCTTGG
GCAGACGTTAAGAAGGATCTTATTTCTTATGGGGGCGGCTGGAAACTGGAAGGCGAATGGAAGGAAGGGG
AGGAGGTGCAGGTGCTGGCCTTGGAACCCGGTAAGAACCCACGAGCAGTGCAGACAAAACCCGGACTTTT
TAAGACTAATGCTGGCACTATCGGCGCGGTGAGCCTTGATTTTTCCCCTGGCACATCTGGGTCCCCCATT
ATTGACAAGAAAGGCAAAGTTGTTGGACTGTATGGAAACGGCGTTGTTACTCGGAGCGGGGCATATGTTT
CTGCAATCGCGCAGACCGAGAAGTCAATCGAAGATAACCCCGAGATAGAGGATGACATTTTCCGAAAAAG
GCGATTGACCATTATGGACTTGCATCCTGGAGCCGGCAAGACAAAGCGGTATCTCCCTGCTATAGTACGC
GAAGCCATTAAACGAGGCCTTAGAACACTGATTCTGGCACCTACAAGAGTTGTTGCTGCAGAAATGGAGG
AAGCCCTCAGGGGACTCCCTATAAGGTACCAGACACCCGCAATAAGAGCAGAGCACACAGGTCGGGAGAT
CGTAGACCTTATGTGTCACGCCACCTTTACAATGAGATTGCTCTCCCCAGTACGGGTGCCGAACTACAAT
CTTATCATTATGGATGAAGCGCATTTTACCGATCCGGCGTCAATTGCTGCAAGAGGATATATCAGCACAC
GAGTGGAGATGGGTGAAGCGGCAGGGATTTTCATGACCGCCACCCCCCCAGGCTCTAGAGACCCTTTTCC
CCAGTCCAACGCACCCATCATAGACGAGGAACGAGAAATTCCCGAGAGATCATGGAACAGCGGGCATGAA
TGGGTGACCGATTTCAAGGGAAAAACAGTGTGGTTTGTCCCTAGCATCAAGGCCGGGAACGATATCGCAG
CCTGCCTCCGGAAAAACGGCAAGAAGGTAATCCAACTGAGTAGGAAGACGTTTGATTCCGAATACGTGAA
GACCCGAACTAATGACTGGGACTTCGTAGTGACGACAGATATTTCAGAGATGGGTGCCAATTTCAAAGCA
GAACGAGTCATTGACCCTAGGAGATGCATGAAGCCGGTAATTCTCACCGATGGAGAAGAGAGGGTAATCC
TGGCCGGGCCCATGCCCGTCACCCATAGCTCTGCGGCCCAGAGACGAGGCAGGATCGGACGGAATCCCAA
GAACGAAAATGACCAATATATTTATATGGGAGAGCCTCTGGAGAACGACGAAGATTGTGCACACTGGAAA
GAGGCCAAGATGCTGCTTGACAACATAAATACACCCGAGGGAATAATTCCAAGCATGTTCGAACCTGAGC
GAGAAAAGGTTGACGCAATTGATGGCGAGTACCGCCTGAGAGGGGAGGCCAGGAAAACATTTGTGGACCT
TATGCGCCGGGGAGATCTTCCCGTCTGGTTGGCCTACCGGGTAGCAGCTGAGGGCATCAACTATGCCGAT
AGGAGATGGTGTTTCGACGGGGTCAAAAATAATCAGATACTGGAAGAAAACGTGGAGGTGGAGATTTGGA
CAAAAGAGGGCGAGAGGAAAAAACTTAAACCTCGGTGGCTTGATGCAAGAATTTATTCCGATCCGTTGGC
CTTGAAGGAGTTTAAAGAATTCGCCGCAGGGAGAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102549 representing NP_739587
Red=Cloning sites Green=Tags

MAGVLWDVPSPPPMGKAELEDGAYRIKQKGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHKGKRIEPSW
ADVKKDLISYGGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTNAGTIGAVSLDFSPGTSGSPI
IDKKGKVVGLYGNGVVTRSGAYVSAIAQTEKSIEDNPEIEDDIFRKRRLTIMDLHPGAGKTKRYLPAIVR
EAIKRGLRTLILAPTRVVAAEMEEALRGLPIRYQTPAIRAEHTGREIVDLMCHATFTMRLLSPVRVPNYN
LIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIIDEEREIPERSWNSGHE
WVTDFKGKTVWFVPSIKAGNDIAACLRKNGKKVIQLSRKTFDSEYVKTRTNDWDFVVTTDISEMGANFKA
ERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRIGRNPKNENDQYIYMGEPLENDEDCAHWK
EAKMLLDNINTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYAD
RRWCFDGVKNNQILEENVEVEIWTKEGERKKLKPRWLDARIYSDPLALKEFKEFAAGRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001474
ORF Size 1857 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001474.2, NP_739587
RefSeq ORF 1857 bp
MW 69.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

其它nonstructural protein NS3产品

Customer Reviews 
Loading...