Gramd1a (BC018554) Mouse Tagged ORF Clone Lentiviral Particle

CAT＃: MR209732L4V

LentiORF^®

Lenti ORF particles, Gramd1a (GFP-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082), 200ul, >10^7 TU/mL

ORF Plasmid: DDK tGFP

Datasheet

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CNY 11,115.00

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经常一起买 (2)

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Specifications

Product Data
Product Name	Gramd1a (BC018554) Mouse Tagged ORF Clone Lentiviral Particle
Synonyms	1300003M23Rik; AI415002; AW547240; D7Bwg0611e
Vector	pLenti-C-mGFP-P2A-Puro
ACCN	BC018554
ORF Size	1941 bp
Sequence Data	ORF Nucleotide Sequence (showhide) The ORF insert of this clone is exactly the same as(MR209732).
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq	BC018554, AAH18554
RefSeq Size	2513 bp
RefSeq ORF	1943 bp
Locus ID	52857
Gene Summary	Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461). May play a role in tumor progression (PubMed:27585821).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
Lenti SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

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