GRK4 (NM_182982) Human Mutant ORF Clone

Specifications

Product Data
Product Name	GRK4 (NM_182982) Human Mutant ORF Clone
Mutation Description	R65L
Affected Codon#	65
Affected NT#	194
Nucleotide Mutation	GRK4 Mutant (R65L), Myc-DDK-tagged ORF clone of Homo sapiens G protein-coupled receptor kinase 4 (GRK4), transcript variant 1 as transfection-ready DNA
Effect	Aiviy, ssoiion wih
Synonyms	GPRK2L; GPRK4; GRK4a; IT11
Vector	pCMV6-Entry
Tag	Myc-DDK
ACCN	NM_182982
ORF Size	1734 bp
Sequence Data	ORF Nucleotide Sequence (showhide) >RC403752 representing NM_182982 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGGAGGATATGGCAAAAAAA GTGGTCGTAGTAAAAAATGGAAGGAGATACTGACACTGCCTCCTGTCAGCCAGTGCAGTGAGCTTAGACA TTCCATTGAAAAGGATTATAGCAGTCTTTGTGACAAGCAACCGATAGGAAGACTTCTCTTCAGGCAGTTC TGTGATACCAAACCCACTCTAAAGAGGCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCG ATGATGAGGACCGAAGTGATTGTGGACTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCC TTTACCAGAAATACCTCCAGATGTTGTGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAA AAAGCCTTTGAGGAATGTACTAGAGTTGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAG AAAGCTCATATTTTTCTCAGTTTTTACAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATT TAGACATTACAGAGTTCTAGGAAAAGGCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGA AAAATGTATGCCTGCAAAAAGCTACAAAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAA ATGAGAAAAGAATTCTGGAGAAAGTGCAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAA AGATGCCTTGTGCTTGGTGCTCACCATTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGC AATCCCGGCTTTGATGAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTAC AGAGGGAAAGAATTGTATACAGAGACTTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCG GATTTCAGACCTCGGTTTGGCCACAGAGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTC GGCTACATGGCACCTGAAGTTGTCAATAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCT GTCTGATCTATGAAATGATTCAGGGACATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGA GGTCGATCAAAGAATCAAGAATGATACCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATC TGCAGGATGTTACTCACCAAGAATCCAAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGA AGCAGCACCCCGTGTTCAAGGACATCAACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTG TCCTGATCCTCATGCCGTTTACTGTAAGGACGTCCTGGATATCGAGCAGTTCTCGGTGGTGAAAGGGATC TACCTGGACACCGCAGATGAAGACTTCTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGA ATGAGATGATCGAATCTGGGTGTTTCAAAGACATCAACAAAAGTGAAAGTGAGGAAGCTTTGCCATTAGA TCTAGACAAGAACATACATACCCCGGTTTCCAGACCAAACAGAGGCTTCTTCTATAGACTCTTCAGAAGA GGGGGCTGCCTGACCATGGTCCCCAGTGAGAAGGAAGTGGAACCCAAGCAATGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGA TAAGGTTTAA Protein Sequence (showhide) >RC403752 representing NM_182982 Red=Cloning site Green=Tags(s) MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRLLFRQF CDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSK KAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATG KMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLG NPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTV GYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSI CRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGI YLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRR GGCLTMVPSEKEVEPKQC SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq	NP_892027
RefSeq Size	1734 bp
RefSeq ORF	1737 bp
Locus ID	2868
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Chemokine signaling pathway, Endocytosis
MW	63.6 kDa
Gene Summary	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

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