Human Myosin Phosphatase (PPP1R12A) activation kit by CRISPRa

CAT#: GA103095

PPP1R12A CRISPRa kit - CRISPR gene activation of human protein phosphatase 1 regulatory subunit 12A



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CNY 12,255.00


货期*
4周

规格
    • 1 kit

Product images

经常一起买 (3)
PPP1R12A Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


PPP1R12A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1
    • 10 ug

CNY 7,272.00


Phospho-Myosin Phosphatase (Thr853) Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Symbol PPP1R12A
Locus ID 4659
Kit Components

GA103095G1, Myosin Phosphatase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

GA103095G2, Myosin Phosphatase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

GA103095G3, Myosin Phosphatase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

1 CRISPRa-Enhancer vector, SKU GE100056

1 CRISPRa scramble vector, SKU GE100077

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
Reference Data
RefSeq NM_001143885, NM_001143886, NM_001244990, NM_001244992, NM_002480
Synonyms M130; MBS; MYPT1
Summary Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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