Mouse Ascl1 activation kit by CRISPRa

CAT#: GA202576

Ascl1 CRISPRa kit - CRISPR gene activation of mouse achaete-scute family bHLH transcription factor 1



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CNY 12,255.00


货期*
4周

规格
    • 1 kit

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Specifications

Product Data
Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Symbol Ascl1
Locus ID 17172
Kit Components

GA202576G1, Ascl1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa, Target Sequence: AGGGAGGGAGCTGAGGAGGT

GA202576G2, Ascl1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa, Target Sequence: GGGAAGAGGAGGGGTAGTGG

GA202576G3, Ascl1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa, Target Sequence: CGGTGCGGCCGCCTTTTCAA

1 CRISPRa-Enhancer vector, SKU GE100056

1 CRISPRa scramble vector, SKU GE100077

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
Reference Data
RefSeq NM_008553
Synonyms AI225900; ASH1; bHLHa46; Mash1
Summary Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways (PubMed:24243019). Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes (PubMed:20107439, PubMed:24243019, PubMed:27281220). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS (PubMed:8217843). Essential for the generation of olfactory and autonomic neurons (PubMed:8221886). Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation (PubMed:16020526, PubMed:17728344).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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