CD7 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CD7
Locus ID	924
Kit Components	KN201231G1, CD7 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGCGAGCCAGCGCCAGAAGC KN201231G2, CD7 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGCCTGGGAAGCTCTTACCT KN201231-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CCTCCCGCCT CCAGGACATG GAACGCAGAG CCACGGTCAG CTGGTCGGCT GCAGCGTTGG GTGCGGGTGC AGGCCCAGGC TCCTTCCCCA CGGCCAAACC AGGGGGTGCC AATTCTGGCC TGCCCTGTGC TCCTCTGCAC TGTGTGGGTG ACTCATGGGT CATGGGTGGC TCCAGATTTC TGGGCATAAA TTGGGTCCCA GCAGAAAGTA CCCCAGAGGA CCAGGCAGGC TGGGGGCCAG CTCTGCCGCT CACTTCCAGA CCCTGCCACC CGTCCCCAGC CAGGGAGTCC CTTCTCTGTC CTCTGGTCTG CCCCGCCCCA GGCCTTGACC TCCTCCCTGT GGAGATGCAG GGGCAGTGGG GGATACAGGG ACGCCCTGCT CTCAGGGCAG CTCTCAGGGA GGCAGTGCTG GGGAGGGGTA GGTGAGACCG CCCCTCCCAC CGGGCCCACA GCACCACCCT GGTCTGAGGC ACCGCCTCCA GGAAGCCCTC TCTGAGCTCT GAGCGCCTGC GGTCTCCTGT GTGCTGCTCT CTGTGGGGTC CTGTAGACCC AGAGAGGCTC AGCTGCACTC GCCCGGCTGG GAGAGCTGGG TGTGGGGAAC AGAGCCCCCA GTCTGAGCCA CAGCACAGCC CCCAGGAAGC GGGTGGGGTG CTGAGCGGCC TCCAGTGTCT GAGGACTCAT TTAAGAGAAG GAAAAAGGGT GGACCCCGGT GGGGAGTGGC CGGGGCTGTC CAGGCAGGGC CGCTGCTTTG GGAGGAAGAA GCCCACAGTC TCGGAACACG AGGACAGCAC CTCCCCCAAC ACCACAGCCG GTGCCCAGAT CTGCTCCATG CCCCGTAAGG CACCGTGTCT TTGGCGACAT GTCAGCCCTG GGCTGTCTCA GGGCCCCACC ATCCCCACCA CTGTCCCCTG CAGGGAGGAC ATTCTCTGTC CTTCTGGCCA GACTGATGGT GACAGCCCAG GTCCTCCCCA GAGGTGCAGC AGTCTCCCCA CTGCACGACT GTCCCCGTGG GAGCCTCCGT CAACATCACC TGCTCCACCA GCGGGGGCCT GCGTGGGATC TACCTGAGGC AGCTCGGGCC ACAGCCCCAA GACATCATTT ACTACGAGGA CGGGGTGGTG CCCACTACGG ACAGACGGTT CCGGGGCCGC ATCGACTTCT CAGGGTCCCA GGACAACCTG ACTATCACCA TGCACCGCCT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_006137
Synonyms	GP40; LEU-9; Tp40; TP41
Summary	This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]

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