SGTA Human Gene Knockout Kit (CRISPR)

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACGAAGA CACACCTCCT CTGCAAGTCC TGTGAAACAG TGATACCAGG GCAGGCACAC CTGGCCCCGT TGGGTACCTA AGAAATGCTT GCTGACATGC CACAAAAGAG GTAAATTGAG GAAAGGGGCA GTGGCTCTGG CTGAGGACAA GGTCTCTCCC TCTCAGCACT GTGGACATTG GGGCTGGATC GTTCTCTGGA GTGGAGCTGT CCTGGGCACT GCAGGGTGCT GGGCAGCATC CCTGGCCCCC ACCCACCTCA TGCCAGAAGC AGGGGCGGAG GGGACCTCCG TGGCGCCTCA GTACCACCCT GGGAGTGCTC AGGAGGCCCT GGGAGCACGC GGGTTCTCTA GGGCAGGCCC TGCTTGCCTG CTGGCCTCAC ACTTTCTGGC TGTGTGCATA GCCGGATTGG TGAGCTCTGG GCCTCAGGTC ATCATCTGTG AAATGGGCGT ATTAAGGCCT TTCTACCTGG GCTGTCCTGG AGCTGAGATC AGCCAGCACC CTTAAAGTGA GGCCCAGCTA GTTAGGGGCA GGCCTGGGGT GCCTGGAGTG GGGAGTGTGA GGGGGCCTGA TGGGGGTTTT TCCTGCACCC CCAGGTATCA CCTCTTCTGG GCTCAAGACT AGCATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAGA ATTCCGATCA TATTCAATAA CCCTTAATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATTAGGTCTG AAGAGGAGTT TACGTCCAGC CAAGCTTAGG ATCTCGACCT CGAAATTCTA CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG GCGCCAACCG ACTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA GGCTGGGAAG GGGTGGGTCC GGGGGCGGGC TCAGGGGCGG GCTCAGGGGC GGGGCGGGCG CCCGAAGGTC CTCCGGAGGC CCGGCATTCT GCACGCTTCA AAAGCGCACG TCTGCCGCGC TGTTCTCCTC TTCCTCATCT CCGGGCCTTT CGACCTGCAT CCATCTAGAT CTCGAGCAGC TGAAGCTTAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCACA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACGCCCGCCC CACGACCCGC AGCGCCCGAC CGAAAGGAGC GCACGACCCC ATGCATCGAT GATATCAGAT CCCCGGGATG CAGAAATTGA TGATCTATTA AACAATAAAG ATGTCCACTA AAATGGAAGT TTTTCCTGTC ATACTTTGTT AAGAAGGGTG AGAACAGAGT ACCTACATTT TGAATGGAAG GATTGGAGCT ACGGGGGTGG GGGTGGGGTG GGATTAGATA AATGCCTGCT CTTTACTGAA GGCTCTTTAC TATTGCTTTA TGATAATGTT TCATAGTTGG ATATCATAAT TTAAACAAGC AAAACCAAAT TAAGGGCCAG CTCATTCCTC CCACTCATGA TCTATAGATC TATAGATCTC TCGTGGGATC ATTGTTTTTC TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGC CTGAAGAACG AGATCAGCAG CCTCTGTTCC ACATACACTT CATTCTCAGT ATTGTTTTGC CAAGTTCTAA TTCCATCAGA AGCTGGTCGA GATCCGGAAC CCTTAATATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TTAGGTCCCT CGAAGAGGTT CACTAGGCGC GCCGAAGGTA GGTGGGTGCC TGCCACTTCC CCCTCTCCCC GGGCAGCGGC CAGCACAGTC GCCTCGTGTA GACACCTGGT GTGTAGATGC CTGGTCCCCC GCCTGGCTTT TTGGGGGTGG AAGCTAAGCC CAGAGAGGGC AGGGGTCTGC CCAGGGCCAC ACAGCCCCCG GCCGTGGGAG CCAGGGCCGA GCTGAGCCCT CCCGCCTGCC ACTGCGTGGC GCCACCACTG CTTGATGCCT TCAGGCTGAG CTGCCTTCCA GTGGCTTTTT TCCATCTCCC CATCTTCCAC CAAGGTGTGT TCTGGAATGT TCTCACCACC CAGTGTGTGT GTGTCACCAT CGGTCACGGG GAAAAAGCTC TCCTAGTGCT GGCCCCGCCC CAGAGTCCCA GACTCGTTGG CATCCTGCAT GGCCTGTCCG CTCCCCAGGT GCCTTGGTCT CCTGGCTGGT CTTGCTTGCG CTTCTGCGAC ACCTGGATCC GGGGCTCGGA GGGAAAGGAG GGCTCGGGGA GGCACCCTCG TGTGGCTGCA GGTTCTGCTC TAGGTCCTCC TTCCCCACCT ATGCCTCGCT CACCTGTGTC CTGGGCCTGC CAGGCCTCTG TAGACGACAG TCTTCTCACT CTCGCCGGTT GGACTTTAGA TCAGAAGGGA TCTTGCTGCC GCCCGAAAGA GGAAGGGCTG GAAGAGGAAG GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC