KRAS Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	KRAS
Locus ID	3845
Kit Components	KN201958G1, KRAS gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTGAATTAGCTGTATCGTCA KN201958G2, KRAS gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAATATAAACTTGTGGTAGT KN201958-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CAATCCAGCT TTATTTGACA CTCATTCTCT CAACTCTCAT CTGATTCTTA CTGTTAATAT TTATCCAAGA GAACTACTGC CATGATGCTT TAAAAGTTTT TCTGTAGCTG TTGCATATTG ACTTCTAACA CTTAGAGGTG GGGGTCCACT AGGAAAACTG TAACAATAAG AGTGGAGATA GCTGTCAGCA ACTTTTGTGA GGGTGTGCTA CAGGGTGTAG AGCACTGTGA AGTCTCTACA TGAGTGAAGT CATGATATGA TCCTTTGAGA GCCTTTAGCC GCCGCAGAAC AGCAGTCTGG CTATTTAGAT AGAACAACTT GATTTTAAGA TAAAAGAACT GTCTATGTAG CATTTATGCA TTTTTCTTAA GCGTCGATGG AGGAGTTTGT AAATGAAGTA CAGTTCATTA CGATACACGT CTGCAGTCAA CTGGAATTTT CATGATTGAA TTTTGTAAGG TATTTTGAAA TAATTTTTCA TATAAAGGTG AGTTTGTATT AAAAGGTACT GGTGGAGTAT TTGATAGTGT ATTAACCTTA TGTGTGACAT GTTCTAATAT AGTCACATTT TCATTATTTT TATTATAAGG CCTGCTGAAA AATCATTTTG TGGACGAATA TGATCCAACA ATAGAGGTAA ATCTTGTTTT AATATGCATA TTACTGGTGC AGGACCATTC TTTGATACAG ATAAAGGTTT CTCTGACCAT TTTCATGAGT ACTTATTACA AGATAATTAT GCTGAAAGTT AAGTTATCTG AAATGTACCT TGGGTTTCAA GTTATATGTA ACCATTAATA TGGGAACTTT ACTTTCCTTG GGAGTATGTC AGGGTCCATG ATGTTCACTC TCTGTGCATT TTGATTGGAA GTGTATTTCA GAGTTTCGTG AGAGGGTAGA AATTTGTATC CTATCTGGAC CTAAAAGACA ATCTTTTTAT TGTAACTTTT ATTTTTATGG GTTTCTTGGT ATTGTGACAT CATATGTAAA GGTTAGATTT AATTGTACTA GTGAAATATA ATTGTTTGAT GGTTGATTTT TTTAAACTTC ATCAGCAGTA TTTTCCTATC TTCTTCTCAA CATTAGAGAA CCTACAACTA CCGGATAAAT TTTACAAAAT GAATTATTTG CCTAAGGTGT GGTTTATATA AAGGTACTAT TACCAACTTT ACCTTTGCTT TGTTGTCATT TTTAAATTTA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_004985, NM_033360, NM_001369786, NM_001369787
Synonyms	C-K-RAS; c-Ki-ras2; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD
Summary	This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

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