USP30 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	USP30
Locus ID	84749
Kit Components	KN202538G1, USP30 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGTCCGCAGGAAGCGCTGGA KN202538G2, USP30 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGCGATGACCGCGGCCGACA KN202538-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TACCTGGCAC CCAAGTAAAC AATAAGTTTG TTGGCTATTA AAGACGTTGA TTTCTCTACT GATATCGTCA GAGATGGGCC TCATGCTCAT TAAAGTTCCT TCCTCTGCGA CCTATTTCCA CTCTGGCCAC AATAATAACT AATTATAGCA GAGGCCTGAC CCGACTTTCG GCTGTTGGCC TCCCTTCCCC ACATCGAAAA ATTCAAATCA TGACCCAGGG TCCATCCTAG AAGTGTGCCA TCTGTATTTA TGAATGGAGC CTAAGCAAAT CTGAAAACCA AGCAGCCCCA GGCAACTGAG CCCAGCGTAG CAACCGACGC CGGGGCCTGT TGCTAAGGGA AAAGAAAGAA AGGACGTGGT CCGTCAGCTA TTGCTCTCCG GGGGCAGCTA CTTCCGGTCC CCCTGGGAGC TGTCCTGCGG TCTACGTTCC CCCGAGGTGC TGGGACTGCG GCCGCAGGTT CCGCTGTCTC GGGAACCGTC GTATCCCTCG GTCCGGCGGC GGCGGCGGCG GTAGCGGAGG AGACGGTTTC AGGCCTCCGG TGCGGCTGCA AAGAGGCCGG GACCAGGGTC CCCAGCTTGG GCCCGTGACG GCTTTTTCAT GCCCTAGTTG GGGTCTCCAG GGCCCGCGCG GGCATTCTGG AAGGTTCTGC GTCCTTTAGG GTTGGAGGCC TGGGCCCGTA GGTGGGAGGA CTGACGGGCT CCAGTCCTTC GGCAACACCA ATTCTGTCTG CACTCCCCAG GTCTGTCAGT CCCTCCCCCT CCTGTCATTG TTCCCAGGAC ACCCGGGGCA CTCCAGTCTT GTCAAGGCCC CTAATTCTGT CAGTGCCCTC AGTCCTTTCA GTGCCTCCTA GGCCTGCCAA GACCCCTCAA TCCTTCTAGT ATACCCCCAG TCCTTCTGGG TCCCCCAATC ATATCAGTTT CTGGACTGCC AGGGTTCCCC AACCTTGGCA CGGTTCTTCT AGTCCTATAA GTGCCCCGGG CCTGCGAGCC CCGCGTTTTG TTAGTACCGC CCCCTATTCC TGTTAGCCCG CCCTCTAGTC TAATATGGAT CGCCACTTCT GTCTGGGTCC CTGATGTTGT TAGTGTGAGT AAAGGCCCTT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001301175, NM_032663
Summary	USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).[supplied by OMIM, Dec 2008]

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