CCN1 (CYR61) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CCN1
Locus ID	3491
Kit Components	KN203492G1, CCN1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACGACTAAGGCGAGCGCCC KN203492G2, CCN1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AACTCACCAGCCTGGTCAAG KN203492-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CACGCCCCAA AGAACCCTTC CCAACATAAG TCGTAATTTA AGGTGGAAAA AACGAACTGT TTTCTTGACG GGTCTGGGAC ACACACACAC ACACACACAC ACACACACAC ACCCCCCGAA CTGTTTTCTT GACGGGTCTG GGAGACAGAC ACACACACAG ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACAAAGGT GCAATGGAGC CAGGGGAGGC GCTTGGCAGC AGCCCGCGCC AACCAGCATT CCTGAGATGT TTGAGAATTC TGGAACGCGC AGACAGAGCC GACGTCACTG CAACACGCGG CGCCTCCGCC GGCCCGTATA AAAGGCGGGC TCCGGGGCGC CTGGGCAGAC CGCGAGCGAG AGCGCCCCCG AGCAGCGCCC GCGCCCTCCG CGCCTTCTCC GCCGGGACCT CGAGCGAAAG ACGCCCGCCC GCCGCCCAGC CCTCGCCTCC CTGCCCACCG GGCCCACCGC GCCGCCACCC CGACCCCGCT GCGCACGGCC TGTCCGCTGC ACACCAGCTT GTTGGCGTCT TCGTCGCCGC GCTCGCCCCG GGCTACTCCT GCGCGCCACA CCCCTTCCCC TGGTCCCAGA TTGCCCACGG CAGGAAAAGT TAAAAAGTTC GCGATCGTTT GCGGGTAGCC GTTTCTTTAA GCACTCTCCC CCTCCCCCCG AAGACGTGTC GGGACCTCTT GGTGGGAGCA GCCTTCCGAG GTGGCCGGGC TGGACGAGAT CAGAGGCTCC CCGTCGATAG GGTCGGAGAC CCCCGTCCCT CACTGCGGCA GCCGCGGCGC CCCTCCTGCG CACCGCGCCG AGTCTCACGC GTATCTTCTC CCCCTTCCAG GCGCTCTCCA CCTGCCCCGC TGCCTGCCAC TGCCCCCTGG AGGCGCCCAA GTGCGCGCCG GGAGTCGGGC TGGTCCGGGA CGGCTGCGGC TGCTGTAAGG TCTGCGCCAA GCAGCTCAAC GAGGACTGCA GCAAAACGCA GCCCTGCGAC CACACCAAGG GGCTGGAATG CAACTTCGGC GCCAGCTCCA CCGCTCTGAA GGGGATCTGC AGAGGTAAGA TGCTTGTGGT TTGGCCCCTT TAAAAAAAAT ACTAGTCCCC ATAGTCCAGA AGTTTAGTAA TTCTGAGACC ATGTATGGTG ATGCTTTGTT GTTGGTAGCT TGGCAGAAAG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001554
Synonyms	CCN1; GIG1; IGFBP10
Summary	The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

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