RBJ (DNAJC27) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	RBJ
Locus ID	51277
Kit Components	KN205500G1, RBJ gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTCCTTCCGCTTCGGCATGT KN205500G2, RBJ gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTCCATGGGCAACGCCGAAG KN205500-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCCAGAACCA GGGTTCCGAC CTCCCCACTT TCCTGGCTTT GGGGTCTGCC TCTCGGGGAT CTCGAGCAAG GGGAACAAAA TATTGGAGGG GGAAGAGGAG CAGCAGCCCG GCCCATGGAA CCGAAAAAGA ATCCAGAAAA AAAAAAAAAC ACTCCCAAAC GGACGAGACA CTACCAGACC CGCCCTGAAG AGCCAATACA TTTCCACCAC TCGGCTGCGA AAGCAACAAA CGCCTCCCGT CCGGGCGTCC CTGAAAACAA GGACGCGCTC ACCTCGAAGG GTAAACCCAG CCGCCCCCCA ACGCCGCCTT CCCGTCAGGG GACGAGGCCT TAGAAACTCG GTAACTGCAG TCGCGTCTCC AGGGCCTGCA GATGACGTCA GCAGCCCCGC GCTAGCATCG CGAGACTCCC GTCTCCCTCC CCCGGCTCCC CACGCTCCCA ACCGCCCGCG CCTGCTGCCA GGCAGCGGCG GGGATGGCGA GCAGCGGAGG CGAGGCGGTG ACGAGAGCAG CGGCTCCGCC ATTGGACGAG GAGGCCTGAG GGACGGGCCA GCGGTGCACA AGAAGAGACC GAGGCGGGTG GCCCCGAGAG AGCCAGGGCC TGGGGAAAGT GAGTACAGCG AGCCGGGAGG GGCGGGCCCC AGCGTGGCGG GGCGACGTGT CCATTGGCTG GAAGGGCGGC CACTCAACAG CCTGGGGCCC GGCCTCCTCT CCGCCCCCGA GCAGGCCCGG GTCTCAGCCG AGGGGTGCGG CTCCGCGAAC GAAACTCCCC GACCTTTTTG ACCTTTCCGT TGCCCCCTCG TCCTCCGACG TCACTCTTCC GGAGATGGTG TCTGGGGTGG CCTCTAAGCC TTGAATGTAC CCCCAAAAGA GTAAGAAGGC CTGGGTCCCC ATGAGGGGCC GTCGGCCTAG ATACCTTGCC CGCGTTCTAG GGGGCCGTCC GGGACCGGGA GATGGAGGAT GAAAGTTTAG TTCCCCCAGC CGGGCCTGGG AGCCGGCGGA ACGTTATGGG GTGCGTGTGA GAATTTGGGG TTCGCGAAAT GGTGCCCGGC AGCCCAGATT CTGCATTCCC GTCACGAGAG AGCGTAACTG ACGTTGACGT GTTTCACAGG GGAGGTGCAA GGGATGGCCG GGGTCCGCGC TGTTTTGTTT TGCGTTGTGT TTTATTTTTG GTGGTGTCCC CATATCTGTT AGTTCCACCG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001198559, NM_016544
Synonyms	RabJS; RBJ
Summary	GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.[UniProtKB/Swiss-Prot Function]

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