Lass5 (CERS5) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Lass5
Locus ID	91012
Kit Components	KN207649G1, Lass5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGACCCCTAAGCTTGCTGTG KN207649G2, Lass5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GACGGCTACGGTTACCCCCG KN207649-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CGGGGCACGA AGGGAAGAAA AGGTTGGGAG CGAAAAGAAC TCTAGTGGCG GACAGTAGTG GGCAGGACCA GCTGACATCC AAGAGAAGCC CTGACCTGAG AGATCGATCT GGCCTCCGGG AGTGGAGACA GAGGTACCGC CTGGGTGGGA GCCGGAACGG ATTCGATTCC AACAGGCTGA GAAAGGAGAA TGTCCCCGTA AAGGAGCTAG GCCTGAAATA AGGGGTGTGG CCGTAGCTGG ATGGAAGAGT GAGGGAGGAG TAGAAGCGTG GCCTAGACCA GCAGCAAAAG AGGGCGGGCC CAGGGCCGCC CGAGGCGTGG TCCCAGGGCC GGGCTGGGCG AGTCAGAGGC TCGGGAAGGG GCGTGGATCC CCGGAGGCGG TCCCCGGGTT GCAGTGAGGG AAGTGGGCCG GGAGGAGAGA TGGGCGGTGA AGAGGCCCGG CCCTGCCAGG GAGGCGGGCG GATCCGTGGC AGTGACCAGA AGGGGCCGGA AGGGGGTGGC CGCCGGCCGG GCCCCGCCCT GGGGCCGCCT CCCCGCGGGT TCCGTTGGCT GTGGCGGCAG CTGACGCTTG TGGCGGCGGT GGCTTCGGGG TGGGCGTAAG CTCGGTGTTC CCGCTGGCGG CGGGCATCTT CTTCGTGAGG CTGCTCTTCG AGCGGTGAGA GCCCGGGAGC GAGCGGCTCG GGGGCCGGGC GCGGGCCGGG GGCGCCCCGC TGTGGGAAGG GCCGAGGGCC GGAAGCAGAA CTGCAAGGAA AGCCCGGAAG CAGGGGCTGG GGGAGCTTGG GCCGCGGCGG GTGATGCTAG GCGCGTGGGC GAGGTGGTAG CCTAGATGGA GAAGGAGTTT GGGGCGTGGG CAAGAGAAAG AGTGAGGGTC TAGGGGAATG CTGGGCCCCT TCAATGTTAG ATCCGGAGTT GGATTGGGGG GGTCATATAG GCCTCCTCCC AACCTCCAAG GGTCCTACAG ATGAGGAAAC AGGTTCAGTG TGAGCTCTGG CAGAGGCCCC GAAGAGAGGG ATAGGAAGGG GCCAGGAGAA CCAGAGCTAG TTGTGACCAT GGAAGCCGAG GGGTCAACGC AAGGGCCAGA CGTTCCAAGG GAAAGGAATG GACTCATCAG ACTCTTAAGA TTTGTTAGGA CGCTTGAGTT TCCGAGAATT GGGGGGAACA GGATTAGTTT GGCCCTAAGA GTTGGTTTGG TATGTCAACT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001281731, NM_001331069, NM_001331070, NM_001331071, NM_001331072, NM_001331073, NM_147190, NR_104035, NR_138532, NR_138533, NR_138534, NR_138535, NR_138536
Synonyms	LASS5; Trh4
Summary	This gene encodes a protein that belongs to the TLC (TRAM, LAG1 and CLN8 homology domains) family of proteins. The encoded protein functions in the synthesis of ceramide, a lipid molecule that is involved in a several cellular signaling pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

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