CXCR2 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CXCR2
Locus ID	3579
Kit Components	KN207794G1, CXCR2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGGGCGGCATCTAGTAGAAA KN207794G2, CXCR2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTGACAGCTTTGAAGATTTC KN207794-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet ATAGCCCATT TGGAGTTCCT GCTTAATCCC CTGATTAAAA ACTGAATGGG GCTGAGTGCA GTGGCTCATG CCTATATTCC CAGCACTTTG GGAGACAGAG GCAGGAGAAT TACTTAAGGT CAGGAGTTTA AGACCATCCT GAGCAACATA ACAAGTCCCC ATCTCTAAGA CAAAAAAAAA AGAACTGAGT GACATCTCAC ATCTCACATT TCTGAACATT AAACCCAGCC TTGATAGCCA AAGATGCTCG CCACTGAAGG ATCCAGGTAG TATTGAGGGT TCTGTGGGGA TTATCCAAAG AGAACTTTCT ACAAAGTTTT AGGTGATGGC GATGCTAAAA GAAATGCTAA GAATTTCTCT CTTATATTAA AGAGAACTAT GGTCCTCTCA TAAAATGTAC CATTTATCAC CAAATTTATC TCATAACCTA AGAGCTACCA CTTACAAATT TGAAGGGAAA AATTACTACA TTGTAATACT CAAGCCAACA CAAAGAATCC TATCCCAGTT TCTTGAGTGG ATGGGCAAGA ATATGGGGAA TTTATTATGC AGTAACCTTC ATCTCTCTTC TATAGGTCAG GATTTAAGTT TACCTCAAAA ATGTGAACCA GAATCCCTGG AAATCAACAA GTATTTTGTG GTCATTATCT ATGCCCTGGT ATTCCTGCTG AGCCTGCTGG GAAACTCCCT CGTGATGCTG GTCATCTTAT ACAGCAGGGT CGGCCGCTCC GTCACTGATG TCTACCTGCT GAACCTAGCC TTGGCCGACC TACTCTTTGC CCTGACCTTG CCCATCTGGG CCGCCTCCAA GGTGAATGGC TGGATTTTTG GCACATTCCT GTGCAAGGTG GTCTCACTCC TGAAGGAAGT CAACTTCTAT AGTGGCATCC TGCTACTGGC CTGCATCAGT GTGGACCGTT ACCTGGCCAT TGTCCATGCC ACACGCACAC TGACCCAGAA GCGCTACTTG GTCAAATTCA TATGTCTCAG CATCTGGGGT CTGTCCTTGC TCCTGGCCCT GCCTGTCTTA CTTTTCCGAA GGACCGTCTA CTCATCCAAT GTTAGCCCAG CCTGCTATGA GGACATGGGC AACAATACAG CAAACTGGCG GATGCTGTTA CGGATCCTGC CCCAGTCCTT TGGCTTCATC GTGCCACTGC TGATCATGCT GTTCTGCTAC GGATTCACCC TGCGTACGCT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001168298, NM_001557
Synonyms	CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB
Summary	The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

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