PTP epsilon (PTPRE) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	PTP epsilon
Locus ID	5791
Kit Components	KN207950G1, PTP epsilon gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ACCAGCAGGAGTGGACACAA KN207950G2, PTP epsilon gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCGCGCAGATCGCGCTGCAG KN207950-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCGCACCAGA AGCGCACGGG GTGCCCTGCG TGTCCCACAC CTGCTGCAAG ACCAGGGCGA GTCCCTAGTC AGGCCACTTC CTGGTGCACA GGGATAGGGC AGGGAGAGGC AGGGCCTTGC CTTTGCTCTC CACCAGGTGT GAGTGCCTGC CATACCCTGA CCCTCTGTCT ACGCACCTGG GTCCCGGCGG AGTGGCTGGA CCAGGCCTCC CCGGAAGGGG CACCAGCCTT GAGTAAACGG ATGCCCACAG TGTCTGGGTG CTCCCAGGGC AGGGCCGCAG GGCCTCAGGG CCATACCCGC CTCGCAGGTG GGGCGGGCGC CCTGGATGCG CTCCCCGCGC CGGGCACCAG GCGTCGCCCC CGCTCCTCGC AGCGCCCAGG CCCCCGCCCC CGCCGCGGAG CCACAGCCGG AGCTGGAGCC GAGGCGGCGG CGGGACGCGG CCGGCCGGAC AAATTTCCTG CTAGGCTGCG GACAGCGGGC GGCAGGAGCC GGCGCGAGCG GCTTCAGGAA CCCACGGCCT CTGCGCGTCC CCGCGACCCT TCTTCGCGCC CGGCGAAGAC AGCCGGGCGC CCCGGAGGGC GGCGGGCAGG CGCCCGGGAG CCTGTGGGGA ACTGTGCACC CCGGGAGGCC CAAGCAGGCC GGGGCGCTGC CTCGGCCGCT GCCGCGGGAG GGAGGGGCCG CTCCGGGGCT CAGAGTCCGG ACCCCGGCCC CGCCGCCCCC GCGGCGCGCC CAGTACCAGC GGCTGGGGCC CGTACGACCC CCGACCCCGG CCTGTGGCGC GTCCCCTCAC CCGGAGTCCC CAGCCCAACT TGGCGACCAC AGTGGCAGTG CTCACGCTCC CTCTCGGTAA ACAGGAGGAG GGCGCGCGTG TCTGCGGGTG GCTACAGTGC GCGGGGGCCG GCGGCAGGGC GGCGTACGTG AAAGCGGGCG ACACAGAGAG GGGGTGCAGG CCGCGCGTGG GGGGCTGCGC CCACTCGAGG CTGGAGGCTG GAGCCTTTGA AGAAAGTGGC TCGAGTTGTG CCAACTCTGG GAGGGGCCGG CGCTCTGCCA GGATGCTGCC CCGCAGCCGG GCGGGCGCCC GCGCCTTCCC AGGGAGGCAG GTGGAGCGGG ATGCAGCAGC CGCCGGGGGT CGGAGATGCG GCAGGGAGAC CCTGGCAGGT GGTCCAGGCT GGACTGCCGC GATTTGCAGG GTCGACGACC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001316676, NM_001316677, NM_006504, NM_130435, NM_001323354, NM_001323355, NM_001323356, NM_001323357
Synonyms	HPTPE; PTPE; R-PTP-EPSILON
Summary	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Several alternatively spliced transcript variants of this gene have been reported, at least two of which encode a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokine-induced SATA signaling, as well as the activation of voltage-gated K+ channels. [provided by RefSeq, Oct 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.