N WASP (WASL) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	N WASP
Locus ID	8976
Kit Components	KN207967G1, N WASP gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ACGTTGGTGACCCTCCGCGG KN207967G2, N WASP gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAGGGACTCGTTCTCCTGCG KN207967-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCAAGCAGCA GTAGCTGGGT TCGGGTACCC AACCGACGAG CCGAGACGCC AGAGGCCAGA GTACGAAGTT GGAAGCCTGC GCCCCAAGCC AACCGGGATT CCACTTAGCT CCGCGCGGTC CGCGCCTCGG CCGGAAGGGG GCGTGGCTTC GGGCCCGCGC CGCGAGGCCC GCCGCCGAGT GGGGGGGTTT CTCCCGGGCC GCAGCCGCGC GTTTCCGGCG CGCTCCCCCG CGCCCTCCGT CCTGTGGTCT CGCCCGCCCC CGCTGCCATG TTGGATTGTG CGGCCGCCGC CGCCGCTGCG GGAGGGTTGG GGGAGGAGTT GGGAGTTTAG CGCAGTCGCC GGAGTGCGAG GACAACGACC ATCCGGCCCT AGCCTGGCCG GGCGGGTGCC GGGAGCTTCC CTTTCTCAGC GCGGCGCGAA GGTGGCTCGC CGTCAGCGCC TGCTTCCCTC GACCTCGTCC TCCTCCCCGC TCCGGAGGAG CTGCGAGATG TGGCGCCTCT GACTCCACTT CTCCCCGCCC CTGTCACCGA GAGGGGGAAC GAGCTCTCGC CCACTCGCCG GAGGAGACGG CCCTGGACTC CCAACCCCGC CGGCGAAACC AAGAAATGTG TGGTCAGTGG ACGAGACCCG CGTCGCCACC CGTGACATTA CCTCACGGGC CCGGGCTTGG GGGCCGGGAA GTGGGAGTGG GGAAGGAGGC GGGAGCGGCG CGGGCCAGGC CTCCCGCCAG CCCCGGCCCC GGCCCCGGCC CCTCGTCGGC GCCCTCGCCC TGCTCCCAGG GTGCGGGCCG TGGCGGTCGG GCGCCGCCTT CCCGAGCTGC TGGAGAATAA GGGAGTGTCA CCTCCGCAGG CCTGTCCTCC AGCCTGGCCG GGCCTCGGCC CGCGCCCTTG CTGCTGGTTA AAACTTTATT CTCAGGCCAC AAGGGGATTT TCTTGCAAGA GCCGCTTGGT CCTAGAATCT GGGCCCTTGT TCTAGCTCTC GCTTCACCTC TTACTGCTTG ATCTGGGGGT ACGTTCCTTA ACAATGCCAG GTTTGAGGAG TGGATAGGTC GGGGAAGGAA GGTGGTTTTT AATCTCCCAT TAGGATTCCA GGAGGGAATG AAGTCGAGTG CAAGAGCCTG GGCTCTCTTC GGATTAGAGA GGGTGCAAGA AATGTTATTG CTCTTCGTTA AAGAGAAACA AGATTTATTT CTCTAGCAAC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_003941
Synonyms	N-WASP; NWASP; WASPB
Summary	This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]

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