KDM1A Human Gene Knockout Kit (CRISPR)

CAT#: KN208480

KDM1A - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


KDM1 Rabbit polyclonal Antibody
    • 100 ul

CNY 2,900.00

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KDM1A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 2
    • 10 ug

CNY 6,240.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol KDM1A
Locus ID 23028
Kit Components

KN208480G1, KDM1A gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGCAGGCGGCTCCGAGAAC

KN208480G2, KDM1A gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCAGCGGCAGCAACCGGGA

KN208480-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

ACCATCGAAA CTGCTGTGCT CTCCAAGTTT TTTGCCCAGT CCAAGCAGGG CAATTATGAA TAAGTAAGAC GCTCTACCAA CATATTTCAG TGTTTTCCCC CATTAGACTG TAAGTTCTTT GAAAGCTAGA ACTTCATAAT GTAACCCTTG TTACGTAGTA GGCAAGCATT AATAAATACT GGTTGAATTA ACGAATAATT GGGTGAGTGA ATGAACGAAC GCACGCATGC AAACCCGAAA GTCCCTGGAG GAAATGGTCA CCTTCGGAGG TTTAGTCTGG CCCAGAAGCC CTAAGACCAC GGACTGTGCC AGGTCCCACT CCAAACGCCG GGGAGACGCT CTAGGCAAGC TACACGTTCT TTGCTGCGGT GCCACTCTAG CCGCGAGAAC GCCGCTCTAT GGCTGCGGGG GAGGGGCGGG GCTCGTGGGT GTCTCCGACC CTTTTTGTCC CGGCGCGGCG GGAGCGCGCT TGGCGCGTGC GTACGCGACG GCGGTTGGCG GCGCGCGGGC AGCGTGAAGC GAGGCGAGGC AAGGCTTTTC GGACCCACGG AGCGACAGAG CGAGCGGCCC CTACGGCCGT CGGCGGCCCG GCGGCCCGAG AGGTGGCCGC GCAGCCCGCG GGCCTGTCGG GCCCAGCCGA GGTCGGGCCG GGGGCGGTGG GGGAGCGCAC ACCCCGCAAG AAAGAGCCTC CGCGGGCCTC GCCCCCCGGG GGCCTGGCGG AACCGCCGGG GTCCGCAGGG CCTCAGGCCG GCCCTACTGT CGTGCCTGGG TCTGCGACCC CCATGGAAAC TGGAATAGCA GAGACTCCGG AGGGGCGTCG GACCAGCCGG CGCAAGCGGG CGAAGGTAAG GCTCGACCCT TCCCTCAAAC GACACCGCCT GGTGCCGAGC TTCCCCGAGG CTTCTCCGCC CTCCCCCGCC GCCGCCGGGT CTTGGGCCCT GCGACCACTC AGACCTCCCC TTGTATCGCT GCGCACGCCA CGTCCCCTCT AGCTGGACGG GTGGGGTGAG AAAGGAAAAG TCTTTGCCGG TGTTGACTGC GGTCTTTGTG CTGGGTCCCG AGCGACGTGG GAAGGGGCAT CGATGATTGC TGGGAGGGGT ATTTTATTCC TTAGGTCTTT CTTGCACACC TTTTCATTTT CTTGACTGGA AAATGGCTCC CTGACGTGGG GTTACGGTTG GCGTTCTTGG AGACCTCACT

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001009999, NM_015013, NM_001363654
Synonyms AOF2; BHC110; CPRF; KDM1; LSD1
Summary This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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