KDM1A Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	KDM1A
Locus ID	23028
Kit Components	KN208480G1, KDM1A gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGCAGGCGGCTCCGAGAAC KN208480G2, KDM1A gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCAGCGGCAGCAACCGGGA KN208480-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet ACCATCGAAA CTGCTGTGCT CTCCAAGTTT TTTGCCCAGT CCAAGCAGGG CAATTATGAA TAAGTAAGAC GCTCTACCAA CATATTTCAG TGTTTTCCCC CATTAGACTG TAAGTTCTTT GAAAGCTAGA ACTTCATAAT GTAACCCTTG TTACGTAGTA GGCAAGCATT AATAAATACT GGTTGAATTA ACGAATAATT GGGTGAGTGA ATGAACGAAC GCACGCATGC AAACCCGAAA GTCCCTGGAG GAAATGGTCA CCTTCGGAGG TTTAGTCTGG CCCAGAAGCC CTAAGACCAC GGACTGTGCC AGGTCCCACT CCAAACGCCG GGGAGACGCT CTAGGCAAGC TACACGTTCT TTGCTGCGGT GCCACTCTAG CCGCGAGAAC GCCGCTCTAT GGCTGCGGGG GAGGGGCGGG GCTCGTGGGT GTCTCCGACC CTTTTTGTCC CGGCGCGGCG GGAGCGCGCT TGGCGCGTGC GTACGCGACG GCGGTTGGCG GCGCGCGGGC AGCGTGAAGC GAGGCGAGGC AAGGCTTTTC GGACCCACGG AGCGACAGAG CGAGCGGCCC CTACGGCCGT CGGCGGCCCG GCGGCCCGAG AGGTGGCCGC GCAGCCCGCG GGCCTGTCGG GCCCAGCCGA GGTCGGGCCG GGGGCGGTGG GGGAGCGCAC ACCCCGCAAG AAAGAGCCTC CGCGGGCCTC GCCCCCCGGG GGCCTGGCGG AACCGCCGGG GTCCGCAGGG CCTCAGGCCG GCCCTACTGT CGTGCCTGGG TCTGCGACCC CCATGGAAAC TGGAATAGCA GAGACTCCGG AGGGGCGTCG GACCAGCCGG CGCAAGCGGG CGAAGGTAAG GCTCGACCCT TCCCTCAAAC GACACCGCCT GGTGCCGAGC TTCCCCGAGG CTTCTCCGCC CTCCCCCGCC GCCGCCGGGT CTTGGGCCCT GCGACCACTC AGACCTCCCC TTGTATCGCT GCGCACGCCA CGTCCCCTCT AGCTGGACGG GTGGGGTGAG AAAGGAAAAG TCTTTGCCGG TGTTGACTGC GGTCTTTGTG CTGGGTCCCG AGCGACGTGG GAAGGGGCAT CGATGATTGC TGGGAGGGGT ATTTTATTCC TTAGGTCTTT CTTGCACACC TTTTCATTTT CTTGACTGGA AAATGGCTCC CTGACGTGGG GTTACGGTTG GCGTTCTTGG AGACCTCACT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001009999, NM_015013, NM_001363654
Synonyms	AOF2; BHC110; CPRF; KDM1; LSD1
Summary	This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

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