IRX2 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	IRX2
Locus ID	153572
Kit Components	KN209142G1, IRX2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCCTGGTACAGGTAGCCCTG KN209142G2, IRX2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTGCCCGGCCTACGGCGCGT KN209142-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TGGCTCCGAG TGGATGGGAG TGGGAGGGGT GCGCGCCGGG GCACGCAGCG CGGCCGGGCT GGGCTGCAGG TCCGCGAGCC GCGGGCTGGG GCCGGAGCTG CTAGAGAGGC GGCTGCGCCA CAAAAGCCTG CCGGTCCCTC CCGCCGCCCG CCTCCGTCGC CTCCGCCCTC CCCCGCCTCC CTCCCGTCTC TCTGCCCCTC CCCCGGCCGC GGCCCGGAGG CTGCAGCGGG CGGAGCAGGG GAGGGGCGGG CCCCCGAATA TGCAGATGAG CGCGTGATGG ACAGCTGCGC GTTCCAATGT CCCTCGGAAA CTCGAGCGCT CCTTCCTCAA CTCCTCACTC CCGACTCCAG ACTCCCCCAA ACCCCGACCG CCGGCCTGGC GCGCGGCTGC GCCCACCGGC TCCGCGCTGC TGCCGCCGCC ACCGCCACCC CGCCGCCGCC CCGGCCCGCG GGCCGCCCAG GCTAGTACCC GGGCCGGCCT GCCTCCGCCG GCCGTCCGGG TGCCCGCCGC CGCGCCGCGC CCCGGCCGGG CGTGGATGGA GGGCGCCGCG CGCCCTGCCC GCTGCTCGGC GTGACGCGGG CCCCGCGCCC CGCGCCCACC CGCGCAGCGA GGAGCTGGCG CGCTCGGCGT CGGGCTCGGC GTTCAGCCCC TACCCGGGCT CGGCGGCCTT CACGGCGCAG GCGGCCACCG GCTTCGGGAG CCCGCTGCAG TACTCGGCCG ACGCCGCCGC CGCCGCCGCC GGCTTCCCGT CCTACATGGT AACCGGGCGC GGGCGGGACG CGGGACTCCT GGGCGCCGGG ACCCAGACGG GGCTCAGGCG GAGCGGGGCT CGGGTTCGGC GGGCGGGGGC CGCCGGGAAT GTGGCGCGGC TGGCGACTCA GGAGTCGCCG GCTCGGTTGG CCGCCCCGCA GGCTGAGCCG GGCCCCGGGT GGGGAGGGCA GCGCGGCAGG CGGGGACGTC CCTGAGCCCG GCCGGGACCC GCGCGCCCCC CGCGCGTCCC CTCGGCTCGC CGGCTGATTT TCTCTGAGAG CCTGGGAAGT GCGTCTCCGC CGGGGCCGGG GTCTGGCGCC GTTTGTCCCT GGTTTCCGCA CCGCAGCCTG GGGAGGCGTT CGGAGCGGGC TGTGTGCTCG CCTGCATTGG CGTGGGGCGC GAGGGTCCGC GTCACTGCGG CCTCTGAGCC GACGGCCGGG CGGTTCGCAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001134222, NM_033267
Synonyms	IRXA2
Summary	IRX2 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.[supplied by OMIM, Apr 2004]

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