MIRO1 (RHOT1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	MIRO1
Locus ID	55288
Kit Components	KN209190G1, MIRO1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAACGTGAGTCCGCACCCTC KN209190G2, MIRO1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAAAGACGTGCGGATCCTGC KN209190-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TTTTCTGACG GCCCTATCCA ATAACGTGGG GACGCAGCTG AAAACCATGC AAGCTGATCG CTGCCCTCCC GGAGTTTATG GTCTGGTGAG AAGATCCCTG CTCTGAGTCA CTGCCAAGCT CTAGCTATGT GACTTTGAAC AGGGAATTTC TGTCCCTATC TATAAAATAA ACGGGGTTAA ACCAGGGAAT CCGCCATTCA AATTCCTGGC CCAAGTGTCT CCGGGGTCGC GGTGCCCAAA GGAGGGTGGG CGAGGCTCCC TGGGCCTGCG CTGCGGCGGC CTGACAGCGG GAGCGCTGTT CCGGCGCAGA GCGCGTTCGC GTTCCGAAAG CGCGTGTCCC TCGCCGCGTG CCGTCGTCCC CGCCGCCGCC GCCGCCGCCG CCGCCGCCGC CGCCGCCGCC GCCGCCGCCA CAGCCCGCTG GGCCGGAGGA GGCGGAGCTG GCGCTGTCCC GGCTCTCTTG CGGGGAAGCA ACTGAGGGGG CGGCGCGGCG GGCCCCGGCG GCCGAAGAGG CTGGCAGGTG GCGCCGTGGG GTGGGTGCTC CTGGTGAGAG GAGTCCACTC CGTGCGTGCG GGCGGAGGCC GGCCCCCGAG AGCCGCCGAC TCCCTGCCCT CCCTGCCCCT GCAGCCCCTG TCGCCCCTGT CGCCCCTGCA GCCCCAACCT TTGCAGCCCC GGCCCTTCTC GCGCCTCACA GCTCCGCTCG CCTTTCTCCG CGTTCCTAGG CCTTCCAGCC CCTTCACTCT TCTCTTTTGG CTGGCCGCCG GCGGCCCTCA CCCTGTCACC TCACCTGGGC CCTCCAGAGA TCTCCTTTCC CTGTTCCCCA GCCGTCCTCC CAAGCCATGT CCCTATTAGC CCTAACCGCC CGACCTCATC CCTCCGAAGC TTCTCTCCTC CTTTTCCTCT TCGAACCCTG TCCTTCTGGG CCTCACAGTT TCCCAGGCCG CCTCCTTTCA GACCTTCTTG TCTTCTGGAG ATTCCCTTAT CACTGCCAGG CAGGTGCTGG AATAAGCAGA CTGACCAATA GGGATTGCGT GAACCGGCTC CCGGGGAAGC TGGGGGAGGG GTGCAGAACT ATTCGACTTG TGGGCAAGGT CAGGTTTCTG CATTTTTTTG GCACTCGCAG TCTTCACTCT CTCTATGTAT TGACATTAAG ATATGAGTAG TGGAAAGGGC CGGATCCTTT TTAGCAGTAC TTGTACTTTG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001033566, NM_001033567, NM_001033568, NM_001288754, NM_001288755, NM_001288758, NM_018307, NR_110083
Synonyms	ARHT1; MIRO-1; MIRO1
Summary	Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.[UniProtKB/Swiss-Prot Function]

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