HGD Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA	mBFP-Neo
Symbol	HGD
Locus ID	3081
Kit Components	KN210009G1, HGD gRNA vector 1 in pCas-Guide CRISPR vector KN210009G2, HGD gRNA vector 2 in pCas-Guide CRISPR vector KN210009BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. Homologous arm and mBFP-Neo sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet GAACCTGAGC TCATTCCTGG GAGGCACCTG CAGAAAACTG GTTCTGGTCC TGGTCCCTGT CTTCAGGTGG CTGTGTGTAA ACATCTTACC CCAGTGTACA CATCTGCAGC TGGTAAATGC CTGGGGGTTT TCTCACTGCT CTCCAGGAGA CTCAGCACTG CCCTGTTCCT TCCTAGCCTG AGTCAGGACT GAGCAGAGAA TCCTCCACCA CACACAGCAG GCACTATCTG CCACAGTTCC TTTCCCCGAT AGCTTCAAAT TCTCTGCCTT TTGAAATAAG CCTACTTTTA ACTGGAATAA ATAATTGGTC AATCTCTACC TCAGGTGAAG AGGAACCAAG CCTCTGGAAA CACTTAGGAA CAAACTGTAA AAACCAAAGG CAATTGTGTA ACCGGTTAAA TAAGCTTGCT GGACTTTGTC CCTGTGTATG AGTTAGACAA TTCTTTCAGC TAGTTTGAGT GACGCACTGA CCAGTGAAGC GCAGTGAAGC AGTGGGAACC GGAATATCCA AAGAGTGGTT TGAAGGAGAA AGAAGCATTG TGGCTTTATA TCCTCTGGGC CTGGGTTTCC TGAAGTCACC ACACATAGAG GAGAGAGAAA GAAAAGACTT AGAGAGGCCC TGGTTCAAAC CCCTGTGTTC AGAGGCTTCC CTGTGGTGTC TGTGGGCACA GCTGGAGCTG AAGAACAAGG GAAAGGTGAT AAAGGAAAGA AATGAAGCTC TTATTGGTCA TCCCTTGCCT TTGTTCATTT CAATTATTGT CTTGGAAATC TGTAGACATC CTAAGAGCTC CCCTCCACTA CGGACATGCA CGTTTCTCTC TATGTCTTTT TCCCAACCCC TGCTCTGACA CCTTCCCATT GCTGCAGCCT CATGGACCAC TTCCGGTGAC CAGTTAGATA TGCTTGATCT ATTTAGTTAA TTAAGGCTCT GAATCAGCCC TTGGCCAGAA GAAGCACCTC CTCTAGGAAA TCATTCCTTC TTTTTAGTAA TACGCAATTG AAAAGTATGT GTGGGTTATG TGCTGTTAGG TGCTAAGCAT TTTAGCTATG AATTAACTCA TAATCCTCAA AATAAATCTG GGGTATAGAG AGAGGTTCTT GTTTCAATTA GAAAAGTAAT GCTTGGAGAG ACTATGATTT GCCCAAGGTC ACTGCCAATA GATGACTTGA TTAGAAGCAG GGTATGTTTG AATGCGAAGT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000187
Synonyms	AKU; HGO
Summary	This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]

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