Tuberin (TSC2) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA	Luciferase-Puro
Symbol	Tuberin
Locus ID	7249
Kit Components	KN211461G1, Tuberin gRNA vector 1 in pCas-Guide CRISPR vector KN211461G2, Tuberin gRNA vector 2 in pCas-Guide CRISPR vector KN211461LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. Homologous arm and Luciferase-Puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet ACGGGGCAGC GGCCTAGAGA GGCGGACCCC GCAGTGTCCG GGTCCCGGGC CCTCACCCGC GCCCACTGCA ACCCGACTCC GGAGCTCCGA GCATCCCTTA GTTTTAAGTC ATGGCGGGTG CGAACGGGTC TCTGCTGCAG GCGGCTCCGT GACAGCTCCT GCTTCACATG GGTAGAGGAG AGACGGCAAA CGTCGGGGCT CCCAGGACTT CGCGGCGGCA GTCCTAACCC GTGCACTGAG TCGGGCGGGG CGGGCGGCAG CGTCTGAGTA GAGAGCTCTC TAGACCAGGC CTGGTGTCTC CCGGGCTTTC CTAGGTGCCT GTTTGCGAGC TGGTCAGCTG GGCTGTAGTT GAGTTCTCCC AGGGAGTGTG GGAGGAAAGG TTATGCCCAC CAGAGACCCA GGGTCCTGAC GGCTGGAGGT CCGCAGTGGG GAAGGTGGGC AGAGGTGTTG CTCAGATGTC CCCATTCCTG TTTCGTTTGC ACAGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCTGCAGAG GGTAAACAGA CGGAGTTTAT CATCACCGCG GAAATACTGA GAGTGAGTGA GCTACCTGTG TCTTTGCTAG GCTAGAGGGA AATGCAGAGA AGGCTGGGTT TGGTCTTGCA CCAGGTTCTG TGGGAGACGG GTTGCTGGCA ACTGTCTACA CAGGAATGCT GTCTCCAGTA CTTGGAGGCC GACATGTCCT TCCTCAGGAG ACTTCGAAAG TCAGGATCTT GGTGACCTGG TTCAGGCACA CTTGAGTTAC TATTCAGATG TTGATGAAAG AAAGCCCATC TTATAGATGA GGCCTGAGTA TCATCATCTT CACAGGTAAG GATATGTCCA GGTGTTACAT GATAAGATGT GAGTTCCTGG GAGAAGTAGG TTCTTTATTT TATTTTTTTT TTGAGACTGA GTCTTGCTCT GTTGCCTAGG CTGGAGTGCA GTGGCACAAT CTCTGCTCGC TGCTACCTCT GCCTCCCAGT TTCAAGTGGT TCTCCTGCCT CAGCCTCCTG AGTAGCTGGG ACTACAGACA CGTGCCACCA CACCCATTTA ATTTTTGTAT TTTTAGTAGA GATGGAGTTT CACTATCTTG GCCAGGCTGG TCT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000548, NM_001077183, NM_001114382, NM_001318827, NM_001318829, NM_001318831, NM_001318832, NM_021055, NM_021056, NM_001363528, NM_001370405, NM_001370404
Synonyms	LAM; PPP1R160; TSC4
Summary	Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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