PTCH1 Human Gene Knockout Kit (CRISPR)

CAT#: KN211551BN

PTCH1 - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Patched1 (Patched) mouse monoclonal antibody, clone OTI5C7 (formerly 5C7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b
    • 10 ug

CNY 15,840.00

Specifications

Product Data
Format 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA mBFP-Neo
Symbol PTCH1
Locus ID 5727
Kit Components

KN211551G1, PTCH1 gRNA vector 1 in pCas-Guide CRISPR vector

KN211551G2, PTCH1 gRNA vector 2 in pCas-Guide CRISPR vector

KN211551BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000264, NM_001083602, NM_001083603, NM_001083604, NM_001083605, NM_001083606, NM_001083607, NM_001354918, NM_001354919, NR_149061
Synonyms BCNS; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11
Summary This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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