HMGCR Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	HMGCR
Locus ID	3156
Kit Components	KN213184G1, HMGCR gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCAAGACTTTTTCGAATGCA KN213184G2, HMGCR gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTTTGTGGCCTCCCATCCC KN213184-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CATCCTTTTG CTAGTGACAT TATGCCATAT GTTCTATGGA ATGAAAAAGT ACAAGAGGTC CCTGCCCTTG AGGATCTTAT CAACTAACAT GATTTATAGC AGGACACTCA ATAAGTGGAT TCTTGGGTGT TTACCTTTTG TGTAATCAGA ATGTAGATGA TGAAGAAGAT ACTTAACATG CATTTTATAT CTAGGTAATT AGAAAATGTG AATAGCTGTT TCTCACTTGT GTTTTCTGCT TGATTGCTCT TCTACTTGCA AGGCTTAGGT AATAAGGTCG AGATACTTAT CTGGTTTGAT CTTAAATGTT TGAATTCATA TAATTTTTAA GAAATGGCTG CTTTAAAGTT GGTTGCCAGT AAGTAATAAA GGATTTATTG TTTGAGTGAA GAAGAAATAA CATAGTTCTC TTAATTTTAT AATTATTTTC CAGAATTATA AGGAACAGTA TCAAATAGTC ATATGTATGG GACACTGTGC ATACAAAGCA GGGTTTATAG CACACTTTTC CTTAAAATCT TTTCCTAAAA ATACAATGAG CTGTATACTA AGTGTTCACC CTTGATATTC CTTCCAGGAT CCAAGGATTC TGTAGCTACA ACCATCTGCA TGATGTCCAT GAACATGTTT ACTGGTAACA ATAAGATCTG TGGTTGGAAT TATGAATGTC CAAAGTTTGA AGAGGTTAGT GAAGTTAATT TGATACTGAC TAAAGTAAAT TACATTTTCA ATTTTTGAAG AGCCCTTAAG CCCCTATAGG GAGCACATAA TTTTTAAAAG TTAGAGTAAA ATATTTATTT TAGTATTTTG GAACTTACCT CAAATTTCTG CTTACATGGA ATGCACTGGA AATGCTCTTA TTTTGCTTTG TCTTTACAAA TGAATGTAAT TGACTTTATT TGAGAAATAC ATCTTTTATA AGTGACTAAT AGTCAAAAAT GATTGTGGGC CGGGTATAGT GACTCATGCC TGTAATCCCA GCACTTTGGG AGGCCAAAGC AGGAGAATTG TTTGAGCCTA GGAGTTCAAG ACCAGCCTGG GCAACCTGGA CAACATAGTA AGACCCAGTC TTTAAAAAAA AATTAAAGGC CGGGCACGGT GGCTCATGTC TGTAATCCCA GCACTTTGGG AGGCCAAGGC GGGTGGATCA CGAGGTCGGG AGATCGAGAC CATCCTGGCT AACATGGTGA AACCCGTGTC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000859, NM_001130996, NM_001364187
Synonyms	LDLCQ3
Summary	HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

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