ATG7 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	ATG7
Locus ID	10533
Kit Components	KN215079G1, ATG7 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGCCCCTTTTAGTAGTGCCT KN215079G2, ATG7 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCGGCAGCTACGGGGGATCC KN215079-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TTGAACCCGG GAGGCGGAGG TTGTAGTGAG CCGAGATTGC ACCACTGCAC TCCAGTGCAA CAGAGTGAGA CTCCATCTCA ATTAAAAAAA AAAAAAATCA CTGTGTCATG AGGAGAATCA GCTTGACAAC ACAGGCCAAT TAGGATGGTT TTCAAAGTTG TGTCTTAAGT GAAATAAGCC AGTCACAAAA ACACAAATAC TGTATGATGC CATTTACATG AAGTAGTCAA ACCTGTATAG ACAGAAAGTA GAATGGTGGG TGCAAGGTCC TGGGGGTAAG GAAATGGGGA GTTATTGTTT AAAGAGTATA GAGTTCCAGT TTTGCAAGAT GAAAAAGTTC TGGAGATCTG TTTCACAACG TTGTGATTAT ACTTAATATC ACCTAACTGT TTACCTCAAA ATAATCAAGA TGGTAAAGTA TATGTTATGT GTTTCTTACC ACAATCCAAA AAGAAGTTGT GTTTCAAGGT AGCCTGTAAA CATCTCATTA CTTTTTGTTT GTTTGAATTA AACTTTATTA CAAATGTTCT TTCTCACCAG GTTTTGCATG GATATGTTAT TTTGCTGTGT TCTGTTTTGT TTTTTAATAG GCAAGAAATA GAAGAAGCTG AACGAGTATC GGCTGGATGA AGCTCCCAAG GACATTAAGG GTTATTACTA CAATGGTAGG TGATTGTAAA TTTCATTTTC CATCATCTTC TGTTATCCTC ACGGTCCTCC AGTATAGTGT CAGCTTTCCT TTAGAAAGAG ACAGCCTTGT CTTTTATAAT CAGTGCTCTT CACTAGTTTC TATTTTACTT CCTGACTTTT TGATCCCAGT TCATTTTCAT TTATTTTCTC TTGTTGTGGG GAAAAAGAAA GAGAGTAGTG AAACTCAAAT TATTAATCCA AATAATACCC ATTACTTAGC ATTGCTGTGG GAAGAGATTG GAAATATTTT TTCCATTGAA TCTTTTTTAT ATTCTTGGCG AATGTCTCAT ATTCACAAGA GCTCTGTCAC ATTCTGCCAT TTATCTATCC ATACATATAT TATCTATGTA GCATAGATAA TCAGAAATTG GATGGGGCGC CTTGGGCTCA ACAAAGAGAA GAAAACTATT CATAAGCATT TTTGTTATGA ACGCTGCTAT TTCTGACTTG TCATTGAAAA TGTGATAATG CTTCTGTCCA GGTGACTCTG CTGGGCTGCC AGCTCGCTTA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001136031, NM_001144912, NM_006395, NM_001349232, NM_001349233, NM_001349234, NM_001349235, NM_001349236, NM_001349237, NM_001349238
Synonyms	APG7-LIKE; APG7L; GSA7
Summary	This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

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