Interferon beta (IFNB1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	Interferon beta
Locus ID	3456
Kit Components	KN218565G1, Interferon beta gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AATTTGGAGGAGACACTTGT KN218565G2, Interferon beta gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCCAAGCAAGTTGTAGCTCA KN218565RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet AATCTGCACA TGTATTTCTG TATATTTTAA ATCATCCCTA GATTACTTAT AATACCTGAT ACAATATAAA TGCTAAATAG CTGTAACACT GTATCTTTAA AATTTACATT ATTTTTTGTT GTTGTATTAT TATTTTTATT GTATTTTTAA AAAATATTTT CCATCTACAG TCAGTAGAAT CCACGGATAC AGAACCTATG GATAGGAAGG ACCAACTGTA TCTTTTAGTG TTTTGAGGTT CTTGAATTCT CAGGTCGTTT GCTTTCCTTT GCTTTCTCCC AAGTCTTGTT TTACAATTTG CTTTAGTCAT TCACTGAAAC TTTAAAAAAC ATTAGAAAAC CTCACAGTTT GTAAATCTTT TTCCCTATTA TATATATCAT AAGATAGGAG CTTAAATAAA GAGTTTTAGA AACTACTAAA ATGTAAATGA CATAGGAAAA CTGAAAGGGA GAAGTGAAAG TGGGAAATTC CTCTGAATAG AGAGAGGACC ATCTCATATA AATAGGCCAT ACCCATGGAG AAAGGACATT CTAACTGCAA CCTTTCGAAG CCTTTGCTCT GGCACAACAG GTAGTAGGCG ACACTGTTCG TGTTGTCAAC AATTTTCAGT GTCAGAAGCT CCTGTGGCAA TTGAATGGGA GGCTTGAATA CTGCCTCAAG GACAGGATGA ACTTTGACAT CCCTGAGGAG ATTAAGCAGC TGCAGCAGTT CCAGAAGGAG GACGCCGCAT TGACCATCTA TGAGATGCTC CAGAACATCT TTGCTATTTT CAGACAAGAT TCATCTAGCA CTGGCTGGAA TGAGACTATT GTTGAGAACC TCCTGGCTAA TGTCTATCAT CAGATAAACC ATCTGAAGAC AGTCCTGGAA GAAAAACTGG AGAAAGAAGA TTTCACCAGG GGAAAACTCA TGAGCAGTCT GCACCTGAAA AGATATTATG GGAGGATTCT GCATTACCTG AAGGCCAAGG AGTACAGTCA CTGTGCCTGG ACCATAGTCA GAGTGGAAAT CCTAAGGAAC TTTTACTTCA TTAACAGACT TACAGGTTAC CTCCGAAACT GAAGATCTCC TAGCCTGTGC CTCTGGGACT GGACAATTGC TTCAAGCATT CTTCAACCAG CAGATGCTGT TTAAGTGACT GATGGCTAAT GTACTGCATA TGAAAGGACA CTAGAAGATT TTGAAATTTT TATTAAATTA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_002176
Synonyms	IFB; IFF; IFNB
Summary	This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection. [provided by RefSeq, Sep 2015]

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