Eg5 (KIF11) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Eg5
Locus ID	3832
Kit Components	KN218842G1, Eg5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTCTTCTTCGCAGACGAATT KN218842G2, Eg5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCCAGGTGGTGGTGAGATGC KN218842-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TGCTTACCAT ATATTCCAAT CTATTTACTT CCACAAGCTC ATTAATTCTC ACCCTAACCC TCTGAGATGG TTACTATTAT TTCCTCTCTG CAGCTGAGGG AATTTTGGGC TAGGGACGTT ATGTAAGTTG AGCCACGCTA CGCTAAAAGT TCCACACTCA ATTCTAGCGT CTCGGCTCTG GACTACCAAG TTCCGGAGCA AGCAGACAGA CCACCTCTTT ACGTTCCCGT AGGCCACGCT CCGGGGGCGG GGCTCCAGTG AGGATACTGC ATCCCATGGT GCCTTGCGCG CCAGCGCAGC CATTGGTCCG GCTACTCTGT CTCTTTTTCA AATTGAGGCG CCGAGTCGTT GCTTAGTTTC TGGGGATTCG GGCGGAGACG AGATTAGTGA TTTGGCGGCT CCGACTGGCG CGGGACAAAC GCCACGGCCA GAGTACCGGG TAGAGAGCGG GGACGCCGAC CTGCGTGCGT CGGTCCTCCA GGCCACGCCA GCGCCCGAGA GGGACCAGGG AGACTCCGGC CCCTGTCGGC CGCCAAGCCC CTCCGCCCCT CACAGCGCCC AGGTCCGCGG CCGGGCCTTG ATTTTTTGGC GGGGACCGTC AGGTAGGGAG AGGGCTGACA GGATTCCGAG CGCTGCGGCT TCGCTGCTGG GCCCCCTACT GCGCGGTCCA GGGAGAGGGA TTTTATTTGC ATTTCCTGAG GGTCCCAGTT TCTTGGTTCT CCGCGTTCTG TTCAATAAAA ATGACACCCG GTTGCTGTGT GTATGTGGTT TTTAGGAGAA AATAACATGT TTGATTTGAT CACTGTTCCA TACTGAAAAG TGCGTTCTTA TGTTTAAACT ATAGTCAATA AAGATGTAGG TGTCACTTTT ATATGCTACT TCATGTAGTT TGTCAGTTTG GAAGTAAGAC TGAATACCTA TTTTGCAGAT GGTTAAGCAC TTACGTTGGT ATTATTACCT CTAAAAAGCA ATCAATCACT GTTCTCTTTA TTCTAGATGC AATATTTTCT GCATCCCTAC TAAACAAGTA AACATCTCTG TCTTTAAACA TTAAAAATTA ATAAAGTGAG TTTATGTTTT TTAAAAGATC AAGACAAGTT AAGCTAGTGT AAATTTCTGA TTGTGCTGCA GTTTTCCCTT TCATCTGCGT GTTTGTTCAT AGACCAAACT AAACGGATAT TTCGAGATAA AGGGATGTGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_004523
Synonyms	EG5; HKSP; KNSL1; MCLMR; TRIP5
Summary	This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.