CD272 (BTLA) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CD272
Locus ID	151888
Kit Components	KN219458G1, CD272 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TAATCCCATATCTGGACATC KN219458G2, CD272 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTGCCTGCCATGCTTGGAAC KN219458-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TAAATCTTCA CTGGTTTAAT GACAATAAGC ATCCTCATCT TACCGTGACA TGTACAGGAA AAAAAAGAAA CCTAAAATCT TTTACATAAA AGAAGTTTCA TAATCACAAG CTATCACAAA GGGGAAAATG TATACTTACC ATTGATAGTT TTACCATAAA TTTCCTGACC TTAATTTGAT TAAAATCTCA ACTTAAAGAC TGCAACAATA CAGATTTCTT TAAAGTTTAA TTGCTCATTT CACTGAACAT TGAGTGCAGG AAAAACCTCA ACCTGGGGCA AAAACGTGGT AGAGCGGAAG GAGAAGTTAA GGACTGAGCT GCTTTGCAGA TCACAGTGTT TGAAAGTGAA CAGCCAACCT GACAAGAAGC AAAAACCACA GAACAGAGTA CCACATCGTC TTTCTGTTCA CTTTTTTTCA CAAAATCATC CAGGCTCTTC CTACTCTCCT CTCTTACCAC CTCTCTCTTC TTTTTTTTTT TTTTTTAGTT ATTTCACAGA TGCCACTGGG GTAGGTAAAC TGACCCAACT CTGCAGCACT CAGAAGACGA AGCAAAGCCT TCTACTTGAG CAGTTTTTCC ATCACTGATA TGTGCAGGAA GTTATTTCTG GTTTTATTTC TCAGGGGGTC TTGGAGGCAA CATTCTCCCT TGCTGAACTT AGTACGGTGT AACGTTTATA CGAAGTGGGT AATCGTAGAT GAAACCAAGC TGTTACATTT GCTTACCGAA GACATCGTAG AGCATAAACC AAATTAGAAC ATAGTATTCA CCTGCCTAAT CATAGGCATC GCATCAATAG CTTGGCTGAC TTTCTTAAAA ACATGTTGAT GGTAGGAAAA GAGAACTGAG TAGATGTTAT AAATAGTTAA GTACAAATCT ATCACTACTT CTTGCAAAAA TGCTCTGGCT GCCATACTGA TAATGAATAG ATAGCTCGTT CTTTCCAAAC TGAGTGCAGT AGAGTGCTGC AGTGCTCAGT TGGGTAGGAC AGTTGTTGGA TAATCGGAAT TATACATATC TCCATGGTTA CCCTCAAATA ATATTACCTC TAAATAAAAT CTCTATACTT GCTTCATTGG GATGTATGCC TTGAGAATAC CGATTGAGAA AATGCTACAA AATATAGGAG CAGAACTTTT CGTCATTGCT TCTATTTTGT TGAAAGGGAA TGGTTCCTAC TTGGTTGTCA ATTGAACACA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001085357, NM_181780
Synonyms	BTLA1; CD272
Summary	This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]

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